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In vivo HAPLOID INDUCTION AND EFFICIENCY OF TWO CHROMOSOME DUPLICATION PROTOCOLS IN TROPICAL MAIZE Ciência e Agrotecnologia
Couto,Evellyn Giselly de Oliveira; Pinho,Édila Vilela de Resende Von; Pinho,Renzo Garcia Von; Veiga,Adriano Delly; Bustamante,Fernanda de Oliveira; Dias,Kaio Olimpio das Graças.
ABSTRACTArtificial chromosome duplication is one of the most important process in the attainment of doubled haploids in maize. This study aimed to evaluate the induction ability of the inducer line KEMS in a tropical climate and test the efficiency of the R1-Navajo marker by flow cytometry to evaluate two chromosome duplication protocols and analyze the development of the doubled haploids in the field. To accomplish this goal, four genotypes (F1 and F2 generations) were crossed with the haploid inducer line KEMS. The seeds obtained were selected using the R1-Navajo marker and subject to two chromosome duplication protocols. Duplication was confirmed using flow cytometry. The percentages of self-fertilized plants after duplication as well as the quantities...
Tipo: Info:eu-repo/semantics/article Palavras-chave: R-Navajo; Colchicine; Induction of maternal haploids; Zea mays.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542015000500435
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