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	Provedor de dados Genet. Mol. Biol. (3) Autor Seijo,Guillermo (3) Robledo,Germán (2) Custódio,Adriana Regina (1) Lavia,Graciela I. (1) Valls,José Francisco Montenegro (1) Palavra-chave RDNA loci (3) Brazil (1) CMA/DAPI bands (1) Chromosome markers (1) Crop wild relatives (1) DAPI bands (1) Dot blot hybridization (1) Genome donors (1) Genome relationships (1) Groundnut (1) Peanut genetic origin (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2013 (1) 2010 (1) 2008 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Genome re-assignment of Arachis trinitensis (Sect. Arachis, Leguminosae) and its implications for the genetic origin of cultivated peanut Genet. Mol. Biol. Robledo,Germán; Lavia,Graciela I.; Seijo,Guillermo. The karyotype structure of Arachis trinitensis was studied by conventional Feulgen staining, CMA/DAPI banding and rDNA loci detection by fluorescence in situ hybridization (FISH) in order to establish its genome status and test the hypothesis that this species is a genome donor of cultivated peanut. Conventional staining revealed that the karyotype lacked the small "A chromosomes" characteristic of the A genome. In agreement with this, chromosomal banding showed that none of the chromosomes had the large centromeric bands expected for A chromosomes. FISH revealed one pair each of 5S and 45S rDNA loci, located in different medium-sized metacentric chromosomes. Collectively, these results suggest that A. trinitensis should be removed from the A genome and be... Tipo: Info:eu-repo/semantics/article Palavras-chave: CMA/DAPI bands; Genome donors; Peanut genetic origin; RDNA loci. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400018 Characterization of the Arachis (Leguminosae) D genome using fluorescence in situ hybridization (FISH) chromosome markers and total genome DNA hybridization Genet. Mol. Biol. Robledo,Germán; Seijo,Guillermo. Chromosome markers were developed for Arachis glandulifera using fluorescence in situ hybridization (FISH) of the 5S and 45S rRNA genes and heterochromatic 4'-6-diamidino-2-phenylindole (DAPI) positive bands. We used chromosome landmarks identified by these markers to construct the first Arachis species ideogram in which all the homologous chromosomes were precisely identified. The comparison of this ideogram with those published for other Arachis species revealed very poor homeologies with all A and B genome taxa, supporting the special genome constitution (D genome) of A. glandulifera. Genomic affinities were further investigated by dot blot hybridization of biotinylated A. glandulifera total DNA to DNA from several Arachis species, the results... Tipo: Info:eu-repo/semantics/article Palavras-chave: Chromosome markers; DAPI bands; RDNA loci; Dot blot hybridization; Genome relationships. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400019 Characterization of Brazilian accessions of wild Arachis species of section Arachis (Fabaceae) using heterochromatin detection and fluorescence in situ hybridization (FISH) Genet. Mol. Biol. Custódio,Adriana Regina; Seijo,Guillermo; Valls,José Francisco Montenegro. The cytogenetic characterization of Arachis species is useful for assessing the genomes present in this genus, for establishing the relationship among their representatives and for understanding the variability in the available germplasm. In this study, we used fluorescence in situ hybridization (FISH) to examine the distribution patterns of heterochromatin and rDNA genes in 12 Brazilian accessions of five species of the taxonomic section Arachis. The heterochromatic pattern varied considerably among the species: complements with centromeric bands in all of the chromosomes (A. hoehnei) and complements completely devoid of heterochromatin (A. gregoryi, A. magna) were observed. The number of 45S rDNA loci ranged from two (A. gregoryi) to eight (A.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Crop wild relatives; Groundnut; RDNA loci; Brazil. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300011 Registros recuperados: 3 Primeira ... 1 ... Última

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