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	Provedor de dados BJMBR (1) Autor Krieger,J.E. (1) Oliveira,E.M. (1) Santos,R.A.S. (1) Palavra-chave ACE activity (1) Angiotensin-converting enzyme (1) Fluorimetric assay (1) Rat tissues (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2000 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Standardization of a fluorimetric assay for the determination of tissue angiotensin-converting enzyme activity in rats BJMBR Oliveira,E.M.; Santos,R.A.S.; Krieger,J.E.. The tripeptide Hip-His-Leu was used to standardize a fluorimetric method to measure tissue angiotensin-converting enzyme (ACE) activity in rats. The fluorescence of the o-phthaldialdehyde-His-Leu adduct was compared in the presence and absence of the homogenate (25 µl) to determine whether the homogenate from different tissues interfered with the fluorimetric determination of the His-Leu product. Only homogenates from lung and renal medulla and cortex showed significantly altered fluorescence intensity. To overcome this problem, the homogenate from these tissues were diluted 10 times with assay buffer. The specificity of the assay was demonstrated by the inhibition of ACE activity with 3 µM enalaprilat (MK-422). There was a linear relationship between... Tipo: Info:eu-repo/semantics/article Palavras-chave: Angiotensin-converting enzyme; ACE activity; Fluorimetric assay; Rat tissues. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000700005 Registros recuperados: 1 Primeira ... 1 ... Última

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