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| Toufiq,Nida; Tabassum,Bushra; Bhatti,Muhammad Umar; Khan,Anwar; Tariq,Muhammad; Shahid,Naila; Nasir,Idrees Ahmad; Husnain,Tayyab. |
| Abstract Agricultural crops suffer many diseases, including fungal and bacterial infections, causing significant yield losses. The identification and characterisation of pathogenesis-related protein genes, such as chitinases, can lead to reduction in pathogen growth, thereby increasing tolerance against fungal pathogens. In the present study, the chitinase I gene was isolated from the genomic DNA of Barley (Hordeum vulgare L.) cultivar, Haider-93. The isolated DNA was used as template for the amplification of the ∼935 bp full-length chitinase I gene. Based on the sequence of the amplified gene fragment, class I barley chitinase shares 93% amino acid sequence homology with class II wheat chitinase. Interestingly, barley class I chitinase and class II... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antifungal activity assay; Barley chitinase class I; Escherichia coli expression; Recombinant chitinase protein. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200414 |
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