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Recovery of phytase produced by solid-state fermentation on citrus peel 52
Rodríguez-Fernández,Daniel Ernesto; Rodríguez-León,José Angel; Carvalho,Júlio Cesar de; Thomaz-Soccol,Vanete; Parada,José Luis; Soccol,Carlos Ricardo.
The extraction of phytase produced by solid-state fermentation of citrus peel was studied employing a multistage leaching process. It was observed that the extracts containing EDTA retained over 90% of phytase activity at room temperature after 24 h after the leaching. A fractional design 2² (with 4 replicates at the central point) was carried out for testing the pH and agitation as process independent factors. Only the interaction between the pH and agitation showed a significant influence. These factors were optimized with a central composite design. Agitation at 300 rpm and pH at 5.0 were the best conditions to extract the enzyme from solid matrix. The modeling of the process indicated that diffusivity of the enzyme in the solvent was the controlling...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Leaching; Recovery multistage; Phytase; Solid state fermentation; Kinetic models.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600026
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