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Registros recuperados: 58 | |
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Rey, Maristela dos Santos; Programa de Pós Graduação em Fitossanidade, Universidade Federal de Pelotas; Pinho, Daiane Schimit de; Porgrama de Pós-Graduação em Biotecnologia, Universidade Federal de Pelotas; Vieira, Anieb Prestes; Iniversidade Federal de Pelotas; Braga, Eugenia Jacira Bolacel; Programa de Pós-Graduação em Fisiologia Vegetal; Pierobom, Carlos Roberto; Programa de Pós-Graduação em Fitossanidade; Peters, José Antônio; Programa de Pós-Graduação em Fisiologia Vegetal, Universidade Federal de Pelotas. |
O objetivo deste estudo foi verificar o efeito do Thidiazuron (TDZ) na regeneração in vitro de mesocótilos de duas cultivares de arroz. Para tal finalidade, os explantes foram inoculados em meios MS contendo diferentes concentrações de TDZ. Os resultados indicam que ocorreu um efeito significativo da concentração do regulador de crescimento em relação à taxa de regeneração e comprimento de brotações. Para a variável comprimento das brotações houve, também, uma interação entre os fatores cultivar e concentração do regulador de crescimento. Para os brotos de primeira passagem observaram-se diferenças significativas na taxa de regeneração das cultivares, bem como entre as doses e interação entre doses e cultivares. Todas as concentrações de TDZ utilizadas... |
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Palavras-chave: 5.01.00.00-9 cultivo in vitro; Regeneração; Regulador de crescimento 5.01.00.00-9 in vitro culture; Regeneration; Growth regulator. |
Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3443 |
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LOPEZ-GARCIA,CARLOS; MOLOWNY,ASUNCION; NACHER,JUAN; PONSODA,XAVIER; SANCHO-BIELSA,FRANCISCO; ALONSO-LLOSA,GREGORI. |
The medial cerebral cortex of lizards, an area homologous to the hippocampal fascia dentata, shows delayed postnatal neurogenesis, i.e., cells in the medial cortex ependyma proliferate and give rise to immature neurons, which migrate to the cell layer. There, recruited neurons differentiate and give rise to zinc containing axons directed to the rest of cortical areas, thus resulting in a continuous growth of the medial cortex and its zinc-enriched axonal projection. This happens along the lizard life span, even in adult lizards, thus allowing one of their most important characteristics: neuronal regeneration. Experiments in our laboratory have shown that chemical lesion of the medial cortex (affecting up to 95% of its neurons) results in a cascade of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Medial cortex; Hippocampus; Postnatal neurogenesis; Neural stem cells; Zinc; Regeneration. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000100006 |
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Gheisari,Yousof; Soleimani,Masoud; Zeinali,Sirous; Arefian,Ehsan; Atashi,Amir; Nikougoftar Zarif,Mahin. |
The kidney has an inherent ability for recovery and regeneration following acute damage. However, there has been much contention as to the source of regenerating renal cells. The aim of this study was to isolate and characterize these cells. Normal rat kidneys were minced and cells were isolated with collagenase I and were cultured in an expansion medium. Adherent cells were isolated and expanded for more than 120 days in vitro. These cells had the potential of translineage differentiation into neural cells, adipocytes and osteocytes. These cells also expressed Nucleostemin, Cyclin D1, Notch1 and Survivin which are commonly expressed in stem cells. The results of the current work show that the adult kidney contains a population of multipotent stem cells. |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Kidney failure; Regeneration; Cell differentiation; Renal replacement therapy. |
Ano: 2009 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452009000100004 |
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ALVAREZ,JAIME. |
In the current understanding of axons, axoplasmic synthesis of proteins is negated, and it is asserted that proteins are transported from perikarya to axons. This 'transport model' in which axons are fully dependent of perikarya is seriously flawed. The 'autonomous axon' proposed here negates in turn transport of proteins, and asserts (i) local synthesis of axoplasmic proteins, corner stone of this model, (ii) existence of internal programs in axon and terminals, (iii) external control of programs resulting in local regulation of phenotype of axons and terminals, hence they are autonomous from perikarya; (iv) participation of perikarya through the fast transport in setting up the axonal programs but not in their immediate regulation. The word merotrophism... |
Tipo: Journal article |
Palavras-chave: Plasticity; Regeneration; Schwann cell; Slow transport; Sprouting; Trophism; Wld s. |
Ano: 2001 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602001000200014 |
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Marzolo,María-Paz; Farfán,Pamela. |
Since the discovery of the low-density lipoprotein receptor (LDLR) and its association with familial hypercholesterolemia in the early 1980s, a family of structurally related proteins has been discovered that has apolipoprotein E as a common ligand, and the broad functions of its members have been described. LRP2, or megalin, is a member of the LDLR family and was initially called gp330. Megalin is an endocytic receptor expressed on the apical surface of several epithelial cells that internalizes a variety of ligands including nutrients, hormones and their carrier proteins, signaling molecules, morphogens, and extracellular matrix proteins. Once internalized, these ligands are directed to the lysosomal degradation pathway or transported by transcytosis... |
Tipo: Journal article |
Palavras-chave: Alzheimer's Disease; Cubilin; Diabetic nephropathy; FXR; Gallstone disease; GSK3; Lowe syndrome; Megalin; PPAR; RAP; Regeneration. |
Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000100012 |
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PATTERSON,SEAN I. |
Protein domains play a fundamental role in the spatial and temporal organization of intracellular signaling systems. While protein phosphorylation has long been known to modify the interactions that underlie this organization, the dynamic cycling of lipids should now be included amongst the posttranslational processes determining specificity in signal transduction. The characteristics of this process are reminiscent of the properties of protein and lipid phosphorylation in determining compartmentalization through SH2 or PH domains. Recent studies have confirmed the functional importance of protein S-palmitoylation in the compartmentalization of signaling molecules that support normal physiological function in cell division and apoptosis, and synaptic... |
Tipo: Journal article |
Palavras-chave: S-palmitoylation; Synapse; Growth cone; Regeneration; Phosphorylation. |
Ano: 2002 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602002000200005 |
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Salm,Rodolfo; Jalles-Filho,Euphly; Schuck-Paim,Cynthia. |
In this study we propose a model that represents the importance of large arborescent palms in the dynamics of seasonally-dry Amazonian forests. Specifically, the model is aimed at guiding the investigation of the role of large arborescent palms on forest regeneration and succession. Following disturbance, the high level of luminosity reaching recently formed forest gaps favors the quick proliferation of shade-intolerant lianas that, by casting shade on the crowns of mature forest trees and increasing tree-fall probability, suppress forest succession. Due to their columnar architecture palm trees are, however, not severely affected by vines. As the palms grow, the canopy at the gaps becomes gradually higher and denser, progressively obstructing the passage... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Amazon; Attalea maripa; Forest dynamics; Palm trees; Regeneration. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032005000300011 |
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Coelho,Irene da Silva; Queiroz,Marisa Vieira de; Costa,Maurício Dutra; Kasuya,Maria Catarina Megumi; Araújo,Elza Fernandes de. |
The aim of this work was to study the standardization of conditions to obtain and regenerate Epulorhiza repens and Ceratorhiza sp. protoplasts. For E. repens, the largest number of protoplasts (8.0 × 10(6) protoplasts/mL) was obtained in 0.6 M KCl, using 15 mg/mL of Lysing Enzymes, and 2-day-old fungal mycelium. When 0.5 M sucrose was used as osmotic stabilizer, the highest frequency of regeneration was achieved (8.5 %); 80.0 % of protoplasts were nucleated, and 20.0 % anucleated. For Ceratorhiza sp., the largest number of protoplasts (4.0 × 10(7) protoplasts/mL) was achieved in 0.6 M NaCl, when 15 mg/mL of Lysing Enzymes and 15mg/mL of Glucanex, with 2-day-old fungal mycelium were used. The highest frequency of regeneration was 6.7 % using 0.5 M sucrose... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ceratorhiza; Epulorhiza; Molecular biology; Protoplast; Regeneration. |
Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100019 |
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Bajpai,Anju; Kalim,Shahina; Chandra,Ramesh; Kamle,Madhu. |
A simple and efficient protocol for recurrent somatic embryogenesis and plant regeneration is one of the prerequisites for genetic improvement of guava. An efficient reproducible regeneration somatic embryogenesis protocol was developed in four genotypes of Psidium guajava L. using immature zygotic embryo as starter explant. Somatic embryogenesis induction was obtained on MS basal medium supplemented with 2.0 mgL-1 2, 4-D, 400 mgL-1 L-glutamine, 6% sucrose and 500 mgL-1 Malt extract. Following SE induction different developmental stages of somatic embryos (Globular, heart-shaped, torpedo, cotyledonary) was directly obtained and further recurrent embryogenesis also obtained upon prolonged incubation in induction media. Addition of polyethylene glycol (50... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Somatic embryogenesis; Zygotic embryo; Recurrent; Maturation; Acclimatization; Induction; Regeneration. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100412 |
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Neves,Valdir Augusto; Lourenço,E. J.. |
Peroxidase from peach fruit was purified 28.9-fold by DEAE-cellulose, Sephadex G-100 and hydroxylapatite chromatography. The purified enzyme showed only one peak of activity with an optimum pH of 5.0 and temperature of 40ºC. The calculated activation energy (Ea) for the reaction was 7.97 kcal/mol. The enzyme was heat-labile in the temperature range of 60 to 80ºC with a fast inactivation at 80ºC. PAGE of the inactivation course at 70ºC showed only one band of activity. Different sugars increased the heat stability of the activity in the following order: sucrose>lactose>glucose>fructose. Measurement of residual activity showed a stabilizing effect of sucrose at various temperature/sugar concentrations (10 to 40%, w/w) with the Ea for inactivation... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Peach peroxidase; Purification; Heat stability; Regeneration. |
Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000200002 |
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Miyabara,E.H.; Aoki,M.S.; Moriscot,A.S.. |
Calcineurin, a Ca2+/calmodulin-dependent phosphatase, is associated with muscle regeneration via NFATc1/GATA2-dependent pathways. However, it is not clear whether calcineurin preferentially affects the regeneration of slow- or fast-twitch muscles. We investigated the effect of a calcineurin inhibitor, cyclosporin A (CsA), on the morphology and fiber diameter of regenerating slow- and fast-twitch muscles. Adult Wistar rats (259.5 ± 9 g) maintained under standard conditions were treated with CsA (20 mg/kg body weight, ip) for 5 days, submitted to cryolesion of soleus and tibialis anterior (TA) muscles on the 6th day, and then treated with CsA for an additional 21 days. The muscles were removed, weighed, frozen, and stored in liquid nitrogen. Cryolesion did... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Calcineurin; Cyclosporin A; Regeneration; Fast and slow twitch muscles. |
Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000400009 |
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Costa,R.A.; Ruiz-de-Souza,V.; Azevedo Jr.,G.M.; Vaz,N.M.; Carvalho,C.R.. |
Round holes in the ears of MRL mice tend to close with characteristics of regeneration believed to be absent in other mouse strains (e.g., C57BL/6). We evaluated the kinetics and the histopathology of ear wound closure in young (8 weeks old) C57BL/6 and BALB/c mice. We also used middle-aged (40 weeks old) C57BL/6 mice to evaluate the influence of aging on this process. A circular through-and-through hole was made in the ear, photographs were taken at different times after injury and wound area was measured with digital analysis software. The percentages of closed area measured on day 100 were: 23.57 ± 8.66% for young BALB/c mice, 56.47 ± 7.39% for young C57BL/6 mice, and 75.31 ± 23.65% for middle-aged C57BL/6 mice. Mice were sacrificed on days 1, 3, 5, 25,... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Wound healing; Regeneration; Scar; Aging; C57BL/6 mice; BALB/c mice. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009001200004 |
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De-Miguel,F.F.. |
Leech neurons in culture have provided novel insights into the steps in the formation of neurite outgrowth patterns, target recognition and synapse formation. Identified adult neurons from the central nervous system of the leech can be removed individually and plated in culture under well-controlled conditions, where they retain their characteristic physiological properties, grow neurites and form specific chemical or electrical synapses. Different identified neurons develop distinctive outgrowth patterns that depend on their identities and on the molecular composition of the substrate. On native substrates, the patterns displayed by these neurons reproduce characteristics from the adult or the developing neurons. In addition, the substrate may induce... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Regeneration; Neurite outgrowth; Synapse formation; Cultured neurons; Leech. |
Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2000000500002 |
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Registros recuperados: 58 | |
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