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Polyphasic characterization of Gluconacetobacterdiazotrophicus isolates obtained from different sugarcane varieties BJM
Guedes,Helma V.; Santos,Samuel T. dos; Perin,Liamara; Teixeira,Kátia R. dos S.; Reis,Veronica M.; Baldani,José I..
A polyphasic approach was applied to characterize 35 G. diazotrophicus isolates obtained from sugarcane varieties cultivated in Brazil. The isolates were analyzed by phenotypic (use of different carbon sources) and genotypic tests (ARDRA and RISARFLP techniques). Variability among the isolates was observed in relation to the carbon source use preference. Glucose and sucrose were used by all isolates in contrast to myo-inositol, galactose and ribose that were not metabolized. The results of the analysis showed the presence of two groups clustered at 68% of similarity. The genetic distance was higher when RISA-RFLP analysis was used. Analysis of 16S rDNA sequences from isolates showed that all of them belonged to the G. diazotrophicus species. Neither effect...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Horizontal spread; Resistance plasmids; ESBL.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400023
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Horizontol dissemination of TEM- and SHV-typr beta-lactamase genes-carrying resistance plasmids amongst clonical isolates of Enterobacteriaceae BJM
Ozgumus,Osman Birol; Tosun,Ilknur; Aydin,Faruk; Kilic,Ali Osman.
The extended-spectrum β-lactamase (ESBL)-producing bacteria have been isolated at increasing frequency worldwide. Expression of ESBL is often associated with multidrug resistance and dissemination by resistance plasmids. During a two-month period in 2000, 133 clinical isolates of enterobacterial strains were randomly collected from outpatients and inpatients at a university hospital in Turkey. The ESBL producing strains were determined by double-disk synergy (DDS) testing. Twenty ESBL producing strains (15%) including Escherichia coli (n = 9), Klebsiella pneumoniae (n = 7), Klebsiella oxytoca (n = 2) and Enterobacter aerogenes (n = 2) were detected and further analyzed for their resistance transfer features, plasmid profile and nature of the resistance...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Horizontal spread; Resistance plasmids; ESBL; Transferência horizontal; ESBL; Plasmídios de resistência.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400007
Registros recuperados: 2
Primeira ... 1 ... Última
 

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