|
|
|
|
|
| Albertoni,Guilherme Ambrozio; Arnoni,Carine Prisco; Araujo,Patricia Regina Barboza; Andrade,Sheila Siqueira; Carvalho,Fabrício Oliveira; Girão,Manoel João Batista Castello; Schor,Nestor; Barreto,José Augusto. |
| Nucleic acid amplification testing (NAT) was recently recommended by Brazilian legislation and has been implemented at some blood banks in the city of São Paulo, Brazil, in an attempt to reduce blood-born transmission of human immunodeficiency virus (HIV) and hepatitis C virus. OBJECTIVE: Manual magnetic particle-based extraction methods for HIV and HCV viral nucleic acids were evaluated in combination with detection by reverse transcriptase - polymerase chain reaction (RT-PCR) one-step. METHODS: Blood donor samples were collected from January 2010 to September 2010, and minipools of them were submitted to testing. ELISA was used for the analysis of anti-HCV/HIV antibodies. Detection and amplification of viral RNA was performed using real-time PCR.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HCV; HIV; Infection; Reverse transcriptase polymerase chain reaction; Blood banks. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702011000600008 |
| |
|
|
| Fica,Alberto; Dabanch,Jeannette; Andrade,Winston; Bustos,Patricia; Carvajal,Ita; Ceroni,Carolina; Triantafilo,Vjera; Castro,Marcelo; Fasce,Rodrigo. |
| Human rhinovirus (HRV) is an emerging viral pathogen. Aim: To characterize a group of patients admitted due to infection by this agent in a general hospital in Chile. Methods: Cases were identified by RT-PCR for 1 year through active surveillance of patients admitted with severe respiratory illness. Diagnosis was not available during hospitalization. Thirty-two cases were identified, 90% were ≥60 years old or had co-morbid conditions. Human rhinovirus-related admissions represented 23.7% of hospitalization due to severe acute respiratory infections among adults and ranked second to influenza (37.8%). Patients presented with pneumonia (68.8%), decompensated chronic lung conditions (21.9%), heart failure or influenza-like illness (6.3% each). Admission to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rhinovirus; Respiratory tract infections; Viral pneumonia; Reverse transcriptase polymerase chain reaction; AdultHospitalization. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000200118 |
| |
|
|
|
