Home Itens selecionados Provedores de dados OAI Créditos Sobre Ajuda

			Busca avançada
	Provedor de dados ArchiMer (3) Autor Le Guyader, Soizick (2) Aouni, M (1) Atmar, Robert (1) Colwell, Rita (1) Elamri, D (1) Estes, Mary (1) Fischer Le Saux, Marion (1) Hervio Heath, Dominique (1) Loaec, Solen (1) Neill, Frederick (1) Parnaudeau, Sylvain (1) Pommepuy, Monique (1) Schwab, Kellogg (1) Mais... Palavra-chave Reverse transcription PCR (3) Artificial seawater (1) Bivalvia (1) Cytotoxin Hemolysin (1) DNA enzyme (1) Detection method (1) Enteric virus (1) Human enteric viruses (1) Immuoessay (1) Molecular detection method (1) Shellfish (1) Vibrio vulnificus (1) Mais... Tipo do documento Text (3) Ano 2006 (1) 2002 (1) 2001 (1) País France (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Detection of human enteric viruses in shellfish collected in Tunisia ArchiMer Elamri, D; Aouni, M; Parnaudeau, Sylvain; Le Guyader, Soizick. Aims: The aim of this study was to detect the main pathogenic human RNA enteric viruses able to persist in the environment such as astrovirus, enterovirus, norovirus and hepatitis A virus (HAV) in shellfish collected from two locations in northern Tunisia. Methods and Results: Viruses were eluted from digestive tissues and concentrated by polyethylene glycol precipitation before nucleic acid extraction and purification. After checking for inhibitors, all viruses were detected by reverse transcription-polymerase chain reaction (RT-PCR) and confirmed by hybridization. Overall, 83% of the samples were found positive for at least one virus. Astrovirus was detected in 61% of the samples, norovirus in 35% and HAV in 26%. Surprisingly, only one sample was found... Tipo: Text Palavras-chave: Shellfish; Reverse transcription PCR; Human enteric viruses. Ano: 2006 URL: http://archimer.ifremer.fr/doc/2006/publication-1881.pdf Development of a reverse transcription-PCR-DNA enzyme immunoassay for detection of "Norwalk-like" viruses and hepatitis A virus in stool and shellfish ArchiMer Schwab, Kellogg; Neill, Frederick; Le Guyader, Soizick; Estes, Mary; Atmar, Robert. Outbreaks of food- and waterborne gastroenteritis are being increasingly reported throughout the world. The analysis of environmental samples by ne,ver diagnostic techniques such as reverse transcription-PCR (RT-PCR) amplification of nucleic acid has begun to identify human enteric viruses (predominantly "Norwalklike" viruses [NLVs]) as the cause of many of these outbreaks. To streamline NLV detection from environmental samples such as shellfish, we have developed an RT-PCR-oligoprobe amplification and detection method using several new procedures that enable confirmed RT-PCR amplification and product detection in 1 day. The new steps include replacing reverse transcriptase and Tag polymerase with rTth polymerase, a heat-stable enzyme that functions as... Tipo: Text Palavras-chave: Bivalvia; Immuoessay; DNA enzyme; Reverse transcription PCR; Detection method; Enteric virus. Ano: 2001 URL: https://archimer.ifremer.fr/doc/2001/publication-1267.pdf Detection of cytotoxin-hemolysin mRNA in nonculturable populations of environmental and clinical Vibrio vulnificus strains in artificial seawater ArchiMer Fischer Le Saux, Marion; Hervio Heath, Dominique; Loaec, Solen; Colwell, Rita; Pommepuy, Monique. The objective of this study was to develop a molecular detection method that better estimates the potential risk associated with the presence of Vibrio vulnificus. For that purpose, we applied seminested reverse transcription-PCR (RT-PCR) to viable but nonculturable (VBNC) populations of V. vulnificus and targeted the cytotoxin-hemolysin virulence gene vvhA. Three strains, two environmental, IF Vv10 and IF Vv18, and one clinical, C7184, were used in this study. Artificial seawater, inoculated with mid-log-phase cells, was maintained at 4degreesC. VBNC cells resulted after 3, 6, and 14 days for C7184, IF Vv18, and IF Vv10, respectively. Our data indicate that seminested RT-PCR is sensitive for the detection of vvhA mRNA in artificial seawater when... Tipo: Text Palavras-chave: Artificial seawater; Reverse transcription PCR; Cytotoxin Hemolysin; Molecular detection method; Vibrio vulnificus. Ano: 2002 URL: https://archimer.ifremer.fr/doc/2002/publication-1269.pdf Registros recuperados: 3 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco