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	Provedor de dados BJMBR (1) Genet. Mol. Biol. (1) Autor Abdelhay,E. (1) Bruno,R.V. (1) Carvalho,J.C. (1) Falco,José Ricardo Penteado (1) Fernandez,Maria Aparecida (1) Rocha,D.N. (1) Saccuti,Cecília Ferreira (1) Soares,Maria Albertina de Miranda (1) Vanario-Alonso,C.E. (1) Mais... Palavra-chave Rhynchosciara americana (2) Axis patterning (1) Bradysia hygida (1) Brazilian flies (1) Dorsal-ventral genes (1) Early embryogenesis (1) Feulgen reaction (1) Genome size (1) Lower dipteran (1) Sciaridae (1) Trichosia pubescens (1) Mais... Tipo do documento Info:eu-repo/semantics/other (2) Ano 2005 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Genome size of three Brazilian flies from the Sciaridae family Genet. Mol. Biol. Saccuti,Cecília Ferreira; Soares,Maria Albertina de Miranda; Falco,José Ricardo Penteado; Fernandez,Maria Aparecida. We determined the genome size of the Brazilian sciarid flies Bradysia hygida, Rhynchosciara americana and Trichosia pubescens (Diptera: Sciaridae) using absorbance measurements of Feulgen-stained nuclei belonging to these species (and chicken erythrocytes as a standard) to calculate the amount of DNA in picograms (pg) and the number of base pairs (bp), or C-value, for each of these species. The C-values were: 3 x 10(8) bp (0.31 pg) for B. hygida; 3.6 x 10(8) bp (0.37 pg) for R. americana; and 1 x 10(9) bp (1.03 pg) for T. pubescens. The sciarids investigated in this work had considerably higher C-values than the average for previously described dipteran species, including D. melanogaster. Tipo: Info:eu-repo/semantics/other Palavras-chave: Genome size; Feulgen reaction; Sciaridae; Bradysia hygida; Rhynchosciara americana; Trichosia pubescens; Brazilian flies. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000500015 Expression of dorsal-ventral genes during early development of Rhynchosciara americana embryos BJMBR Carvalho,J.C.; Rocha,D.N.; Bruno,R.V.; Vanario-Alonso,C.E.; Abdelhay,E.. The establishment of dorsal-ventral polarity in Drosophila is a complex process which involves the action of maternal and zygotically expressed genes. Interspecific differences in the expression pattern of some of these genes have been described in other species. Here we present the expression of dorsal-ventral genes during early embryogenesis in the lower dipteran Rhynchosciara americana. The expression of four genes, the ventralizing genes snail (sna) and twist (twi) and the dorsalizing genes decapentaplegic (dpp) and zerknüllt (zen), was investigated by whole-mount in situ hybridization. Sense and antisense mRNA were transcribed in vitro using UTP-digoxigenin and hybridized at 55°C with dechorionated fixed embryos. Staining was obtained with... Tipo: Info:eu-repo/semantics/other Palavras-chave: Rhynchosciara americana; Lower dipteran; Axis patterning; Dorsal-ventral genes; Early embryogenesis. Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000100005 Registros recuperados: 2 Primeira ... 1 ... Última

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