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	Provedor de dados BJM (1) Autor Ansarypour,Zahra (1) Shahpiri,Azar (1) Palavra-chave Cadmium (1) Ethanol (1) Hydrogen peroxide (1) Rice metallothionein (1) S. cerevisiae (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2017 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Heterologous expression of a rice metallothionein isoform (OsMTI-1b) in Saccharomyces cerevisiae enhances cadmium, hydrogen peroxide and ethanol tolerance BJM Ansarypour,Zahra; Shahpiri,Azar. Abstract Metallothioneins are a superfamily of low-molecular-weight, cysteine (Cys)-rich proteins that are believed to play important roles in protection against metal toxicity and oxidative stress. The main purpose of this study was to investigate the effect of heterologous expression of a rice metallothionein isoform (OsMTI-1b) on the tolerance of Saccharomyces cerevisiae to Cd2+, H2O2 and ethanol stress. The gene encoding OsMTI-1b was cloned into p426GPD as a yeast expression vector. The new construct was transformed to competent cells of S. cerevisiae. After verification of heterologous expression of OsMTI-1b, the new strain and control were grown under stress conditions. In comparison to control strain, the transformed S. cerevisiae cells expressing... Tipo: Info:eu-repo/semantics/article Palavras-chave: Cadmium; Ethanol; Hydrogen peroxide; Rice metallothionein; S. cerevisiae. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300537 Registros recuperados: 1 Primeira ... 1 ... Última

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