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| Horta,M.C.; Pinter,A.; Cortez,A.; Soares,R.M.; Gennari,S.M.; Schumaker,T.T.S.; Labruna,M.B.. |
| Samples of 10 and 14 Ctenocephalides felis felis fleas were collected on dogs from Pedreira and Mogi das Cruzes municipalities, respectively, in the State of São Paulo, Brazil, for detection of Rickettsia spp. Individual fleas were submitted to Polymerase Chain Reaction targeting the 17-kDa and the 190-kDa (OmpA) genes of Rickettsiae. This later gene is specific for spotted fever group. Nine fleas from Pedreira (90%) and four fleas from Mogi das Cruzes (28%) were positive for the 17-kDa gene, and eight fleas from Pedreira (80%) and four from Mogi das Cruzes (28%) were positive for 190-kDa gene. The nucleotide sequence of the 190-kDa products of one flea from Pedreira and one flea from Mogi das Cruzes were 100% identical to each other, and when compared to... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rickettsia felis; Ctenocephalides felis felis; Dog; 190-kDa gene; Brazil. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000300008 |
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| Horta,Mauricio C.; Scott,Fabio B.; Correia,Thaís R.; Fernandes,Julio I.; Richtzenhain,Leonardo J.; Labruna,Marcelo B.. |
| The present study evaluated the rickettsial infection in a laboratory colony of cat fleas, Ctenocephalides felis felis (Bouche) in Brazil. All flea samples (30 eggs, 30 larvae, 30 cocoons, 30 males, and 30 females) tested by polymerase chain reaction (PCR) were shown to contain rickettsial DNA. PCR products, corresponding to the rickettsial gltA, htrA, ompA and ompB gene partial sequences were sequenced and showed to correspond to Rickettsia felis, indicating that the flea colony was 100% infected by R. felis. The immunofluorescence assay (IFA) showed the presence of R. felis-reactive antibodies in blood sera of 7 (87.5%) out of 8 cats that were regularly used to feed the flea colony. From 15 humans that used to work with the flea colony in the laboratory,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rickettsia felis; Flea; Ctenocephalides felis felis; Cats; Humans. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300035 |
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