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| Ottone,Nicolás Ernesto; Cirigliano,Vanina; Lewicki,Marianela; Bianchi,Homero F; Aja-Guardiola,Santiago; Algieri,Rubén Daniel; Cantin,Mario; Fuentes,Ramón. |
| Today, alternatives methods are developed for the use of laboratory animals for teaching, research and surgical training. In our work we present a novel alternative to the use of rats, by developing a technique of plastination at room temperature. High-quality rat preparations from the anatomical dissection point of view were obtained, in order to indefinitely preserve them dry, the thoracic and abdominal organs conserve its natural volume and shape, maintaining their texture and color. No odors or hassles and toxic vapors of conventional preserving agents were found. This technique allows the collection of dry, completely biosafe and durable specimens in a short time and with excellent quality. Plastination in laboratory rats complements undergraduate and... |
| Tipo: Journal article |
Palavras-chave: Plastination; Room temperature; Laboratory animals; Rats. |
| Ano: 2014 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022014000400048 |
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| Bergstein-Galan,T.G.; Weiss,R.R.; Barbosa,T.S.R.; Kozicki,L.E.; Bicudo,S.D.. |
| ABSTRACT The objectives of this study were to verify the time during which viable ovine spermatozoa could be recovered from the cauda epididymis kept at ambient temperature (18-25°C). Sperm collected in an artificial vagina (AV) were used as control. Spermatozoa samples were collected with an AV and from epididymis at 0 (G0), 6 (G6), 12 (G12), 24 (G24), and 48 (G48) hours post mortem. Total motility (TM), progressive motility (PM), hypo-osmotic membrane integrity test (HOST) and morphological changes were assessed. TM decreased (P<0.05) from 24 hours post mortem (70.0±1.9%) compared to AV (86.4±1.0%). PM decreased (P<0.05) from 12 hours after death (31.3±4.0%) compared to AV group (73.2±1.4%). The percentage of viable cells in HOST decreased... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ram; Epididymis; Room temperature. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401023 |
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| Bertol,Melina Andrea Formighieri; Weiss,Romildo Romualdo; Thomaz-Soccol,Vanete; Kozicki,Luiz Ernandes; Fujita,Aline Silva; Abreu,Renata Azevedo de; Green,Kerriel Thandile. |
| The aim of this study was to evaluate the viability of bull spermatozoa collected from the cauda epididymis stored at 18-20°C, which were compared with semen collected by electro-ejaculation method and preserved at 5°C. Ten pairs of testes from Tabapuã bulls were removed by orchiectomy and stored for 6 (G6), 12 (G12), 18 (G18), 24 (G24) and 30 (G30) h at room temperature (18-20°C). Seven days before orchiectomy, semen was collected by electro-ejaculation method. The sperm parameters evaluated were: sperm motility, vigor, concentration, morphology and acrosome defects. Sperm motility declined (p<0.05) when spermatozoa were stored for 30 h in the epididymis. The spermatozoa from the epididymis showed lower sperm motility than that of spermatozoa collected... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bull; Epididymis; Orchiectomy; Sperm; Room temperature; Viability. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500008 |
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| França,C.N.; Mendes,C.C.; Ferreira,C.E.S.. |
| The stability of samples is crucial for getting reliable concentrations of many analytes, including lipid profile. Thus, the goal of this study was to analyze lipid profile under different storage and temperature conditions. This was a prospective study with 809 patients of both genders. Total cholesterol, triglycerides, high-density lipoprotein cholesterol, low density lipoprotein cholesterol and non-high-density lipoprotein were measured within 1 h from collection at room temperature, after 2-3 h of refrigeration (8°C) and after 4-5 h at room temperature. The processing time and storage conditions did not affect the analytes measured. These findings are important for multicenter studies, because of the difficulties related to centrifugation and freezing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Lipid profile; Storage; Temperature; Stability; Refrigerator; Room temperature. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000300608 |
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| Souza,M.M.; Boyle,R.T.. |
| Chick cardiomyocytes, when submitted to hyposmotic swelling, exhibit a partial regulatory volume decrease (RVD). A Ca2+ influx by stretch-activated channels signals a taurine efflux and the RVD at 37°C. We evaluated the cell's performance at room temperature. Cardiomyocytes isolated and cultured from 11-day-old chick embryos were submitted to a hyposmotic solution (180 mOsm/kg H2O) at 37°C and at room temperature (26°C). Under these conditions we measured the changes in cell volume as well as the intracellular free Ca2+ (using fura-2). During hyposmotic swelling, cells at 37°C displayed a peak relative volume of 1.61 ± 0.03 and recovery to 1.22 ± 0.04 (N = 14), while cells at 26°C presented a peak swell relative volume of 1.74 ± 0.06 and did not recover... |
| Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Cell volume; RVD; Room temperature; High free calcium. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000100018 |
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