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Identification and characterization of rhizospheric microbial diversity by 16S ribosomal RNA gene sequencing BJM
Naveed,Muhammad; Mubeen,Samavia; khan,SamiUllah; Ahmed,Iftikhar; Khalid,Nauman; Suleria,Hafiz Ansar Rasul; Bano,Asghari; Mumtaz,Abdul Samad.
In the present study, samples of rhizosphere and root nodules were collected from different areas of Pakistan to isolate plant growth promoting rhizobacteria. Identification of bacterial isolates was made by 16S rRNA gene sequence analysis and taxonomical confirmation on EzTaxon Server. The identified bacterial strains were belonged to 5 genera i.e. Ensifer, Bacillus, Pseudomona, Leclercia and Rhizobium. Phylogenetic analysis inferred from 16S rRNA gene sequences showed the evolutionary relationship of bacterial strains with the respective genera. Based on phylogenetic analysis, some candidate novel species were also identified. The bacterial strains were also characterized for morphological, physiological, biochemical tests and glucose dehydrogenase (gdh)...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rhizosphere; Root nodules; 16S rRNA gene sequence; Glucose dehydrogenase; Pyrroloquinolone quinone.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300031
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Identification and characterization of the endophytic plant growth prompter Bacillus Cereus strain mq23 isolated from Sophora Alopecuroides root nodules BJM
Zhao,Longfei; Xu,Yajun; Sun,Ran; Deng,Zhenshan; Yang,Wenquan; Wei,Gehong.
Endophytes MQ23 and MQ23R isolated from Sophora alopecuroides root nodules were characterized by observing their ability to promote plant growth and employing molecular analysis techniques. Results showed that MQ23 and MQ23R are potential N2-fixing endophytes and belong to the same species as Bacillus cereus. MQ23 was shown to be able to produce siderophores, IAA, and demonstrate certain antifungal activity to plant pathogenic fungi. Co-inoculation with MQ23+MQ23II showed a more significant effect than inoculation alone in vitro for most of positive actions suggesting they have a cooperative interaction. Results of plant inoculation with endophytes indicated that the growth indexes of co-inoculated MQ23+MQ23II were higher than those of inoculated alone...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Plant growth promoting characterization; Sophora alopecuroides; Root nodules; Endophytic bacteria; Bacillus cereus.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000200022
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