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Influence of static magnetic fields on S. cerevisae biomass growth BABT
Muniz,João B.; Marcelino,Milton; Motta,Mauricio da; Schuler,Alexandre; Motta,Mauricy Alves da.
Biomass growth of Saccharomyces cerevisiae DAUFPE-1012 was studied in eight batch fermentations exposed to steady magnetic fields (SMF) running at 23ºC (± 1ºC), for 24 h in a double cylindrical tube reactor with synchronic agitation. For every batch, one tube was exposed to 220mT flow intensity SMF, produced by NdFeB rod magnets attached diametrically opposed (N to S) magnets on one tube. In the other tube, without magnets, the fermentation occurred in the same conditions. The biomass growth in culture (yeast extract + glucose 2%) was monitored by spectrometry to obtain the absorbance and later, the corresponding cell dry weight. The culture glucose concentration was monitored every two hours so as the pH, which was maintained between 4 and 5. As a result,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: S. cerevisiae; Biomass; Magnetic fields; Fermentation.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000300017
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Susceptibility of Saccharomyces cerevisiae and lactic acid bacteria from the alcohol industry to several antimicrobial compounds BJM
Oliva-Neto,Pedro de; Yokoya,Fumio.
The antimicrobial effect of several products including commercial formulations currently used in sugar and alcohol factories was determined by adapted MIC (Minimal Inhibitory Concentration) test on Saccharomyces cerevisiae and on natural contaminants Lactobacillus fermentum and Leuconostoc mesenteroides. The MIC test by macrodilution broth method was adapted by formulating of the culture medium with cane juice closely simulating industrial alcoholic fermentation must. Acid penicillin V (MIC 0.10-0.20 µg/ml) and clindamycin (MIC 0.05-0.40 µg/ml) were most effective against bacterial growth in 24 h. Among the chemicals, sulphite (MIC 10-40 µg/ml), nitrite (MIC <117 µg/ml) and copper sulphate (75-300 µg/ml) were the most effective. Zinc and manganese...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Antimicrobial compound; MIC; S. cerevisiae; Lactic acid bacteria.
Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000100003
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Characteristics of Saccharomyces cerevisiae yeasts exhibiting rough colonies and pseudohyphal morphology with respect to alcoholic fermentation BJM
Reis,Vanda Renata; Bassi,Ana Paula Guarnieri; Silva,Jessica Carolina Gomes da; Ceccato-Antonini,Sandra Regina.
Among the native yeasts found in alcoholic fermentation, rough colonies associated with pseudohyphal morphology belonging to the species Saccharomyces cerevisiae are very common and undesirable during the process. The aim of this work was to perform morphological and physiological characterisations of S. cerevisiae strains that exhibited rough and smooth colonies in an attempt to identify alternatives that could contribute to the management of rough colony yeasts in alcoholic fermentation. Characterisation tests for invasiveness in Agar medium, killer activity, flocculation and fermentative capacity were performed on 22 strains (11 rough and 11 smooth colonies). The effects of acid treatment at different pH values on the growth of two strains ("52" -rough...
Tipo: Info:eu-repo/semantics/article Palavras-chave: S. cerevisiae; Ethanol; Fermentation; Indigenous yeasts.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400014
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Heterologous expression of a rice metallothionein isoform (OsMTI-1b) in Saccharomyces cerevisiae enhances cadmium, hydrogen peroxide and ethanol tolerance BJM
Ansarypour,Zahra; Shahpiri,Azar.
Abstract Metallothioneins are a superfamily of low-molecular-weight, cysteine (Cys)-rich proteins that are believed to play important roles in protection against metal toxicity and oxidative stress. The main purpose of this study was to investigate the effect of heterologous expression of a rice metallothionein isoform (OsMTI-1b) on the tolerance of Saccharomyces cerevisiae to Cd2+, H2O2 and ethanol stress. The gene encoding OsMTI-1b was cloned into p426GPD as a yeast expression vector. The new construct was transformed to competent cells of S. cerevisiae. After verification of heterologous expression of OsMTI-1b, the new strain and control were grown under stress conditions. In comparison to control strain, the transformed S. cerevisiae cells expressing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cadmium; Ethanol; Hydrogen peroxide; Rice metallothionein; S. cerevisiae.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300537
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High levels of catalase in sod mutants of Saccharomyces cerevisiae in high aeration conditions BJM
Martins,Vanessa; Manfredini,Vanusa; Benfato,Mara Silveira.
Saccharomyces cerevisiae mutants deficient in superoxide dismutase genes (sod1delta, sod2delta and sod1deltasod2delta mutants) in a stationary phase of growth under high aeration conditions were subjected to H2O2 stress. All the mutants were sensitive after H2O2 treatment. Glutathione peroxidase levels were significantly lower in sod1delta and sod2delta single mutants than in the wild-type without treatment. After exposure to H2O2 concentrations, glutathione peroxidase levels were increased in sod1deltasod2delta double mutants and the sod2delta single mutant, while sod1delta maintained lower gluthatione peroxidase activities. The sod2delta mutant demonstrated a similar catalase activity to that of the wild-type without treatment, whilst decreased catalase...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Catalase; Superoxide dismutase; High aeration; Hydrogen peroxide; S. cerevisiae.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400008
Registros recuperados: 5
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