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Potential application of protease isolated from Pseudomonas aeruginosa PD100 Electron. J. Biotechnol.
Najafi,Mohsen Fathi; Deobagkar,Dileep; Deobagkar,Deepti.
A protease isolated from Pseudomonas aeruginosa PD100 could act in the presence of SDS and Tween 80. This protease could be useful for degradation of protein in the presence of solvent, dehairing of cow skin and degradation of natural proteins. The immobilized protease showed 15-20% increases in temperature stability and the entrapped enzyme retained 83% of its initial activity after six cycles. With respect to properties of the enzyme and its capability for degradation of different protein sources, this protease finds potential application for waste treatment, used in detergents and leather industry
Tipo: Journal article Palavras-chave: Blood clot; Protease; Pseudomonas aeruginosa PD100; SDS; Solvent.
Ano: 2005 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000200009
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Inoculation methods under greenhouse conditions for evaluating soybean resistance to sudden death syndrome PAB
Farias Neto,Austeclinio Lopes de; Schmidt,Michael; Hartman,Glen Lee; Shuxian,Li; Diers,Brian Willians.
The objectives of this work were to evaluate two greenhouse screening methods for sudden death syndrome (SDS) and to determine which one is best correlated with field resistance of soybean genotypes. The evaluations were done with three sets of genotypes that were classified as partially resistant, intermediate, and susceptible to SDS based on previous field evaluations. These three sets were independently evaluated for greenhouse SDS reactions using cone and tray inoculation methods. Plants were infected using grains of white sorghum [Sorghum bicolor (L.) Moench] infested with Fusarium solani f. sp. glycines. Foliar symptom severity was rated 21 days after emergence. The cone and field SDS ratings were significantly correlated and ranged from 0.69 for set...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Fusarium solani; Fusarium virguliforme; Cone inoculation method; SDS; Symptom severity; Tray inoculation method.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001100005
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Identification of quantitative trait loci for resistance against soybean sudden death syndrome caused by Fusarium tucumaniae PAB
Yamanaka,Naoki; Fuentes,Francisco Horacio; Gilli,Javier Ramon; Watanabe,Satoshi; Harada,Kyuya; Ban,Tomohiro; Abdelnoor,Ricardo Vilela; Nepomuceno,Alexandre Lima; Homma,Yoshihisa.
The objective of this work was to identify genomic regions that underlie resistance to Fusarium tucumaniae sp. nov., the causing agent of sudden death syndrome (SDS) in soybean in South America, using a population with a genetic background different from that previously reported for Fusarium virguliforme sp. nov. (F. solani f. sp. glycines), also responsible for SDS in soybean. Although major genes and quantitative trait loci (QTL) for SDS resistance have been identified, little is known about the same disease caused by Fusarium tucumaniae sp. nov., in South America. To identify genetic factors related to resistance to F. tucumaniae and DNA markers associated with them, a QTL analysis was performed using recombinant inbred lines. The map locations of the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Glycine max; Disease index; Residual heterozygous line; SDS.
Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006000900006
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Use of Near Infrared Reflectance Spectroscopy to Evaluate Quality Characteristics in Whole-Wheat Grain Agricultura Técnica
Cozzolino,Daniel; Delucchi,Inés; Kholi,Moham; Vázquez,Daniel.
The aim of this work was to explore the potential of visible (Vis) and near infrared reflectance (NIR) spectroscopy to measure quality characteristics in whole grain wheat (Triticum aestivum L.) as a tool in breeding programs. A total of 100 samples were analyzed by the reference methods for crude protein (CP), wet gluten (WG) and sodium dodecyl sulfate (SDS) sedimentation test. Whole grain samples were scanned in a NIR monochromator instrument (400-2500 nm) in reflectance. Partial least squares (PLS) were used to develop calibration equations for the quality characteristics in whole wheat. Calibration models were validated using an independent set of samples (n = 50) randomly selected from the population set. The uncertainty of the PLS models was...
Tipo: Journal article Palavras-chave: Whole wheat; NIR; Protein; Wet gluten; Grain quality; SDS.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0365-28072006000400005
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