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	Provedor de dados Acta biol.Colomb. (1) BABT (1) Autor Astray,Renato Mancini (1) Costa,Bruno Labate Vale da (1) Jorge,Soraia Attie Calil (1) Monteiro,Daniella Cristina Ventini (1) NAVAS,Maria-Cristina (1) Núñez,Eutimio Gustavo Fernández (1) PAVLOVIC,Jovan (1) Pereira,Carlos Augusto (1) Rezende,Alexandre Gonçalves de (1) STOLL-KELLER,Françoise (1) Tonso,Aldo (1) Mais... Palavra-chave SFV (2) BHK-21 (1) Dendritic cells (1) HCV (1) Large-scale bioprocesses (1) Mammalian cells (1) Recombinant proteins (1) Transient expression (1) Viral vector (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2019 (1) 2013 (1) País Brazil (1) Colombia (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última LACK OF EXPRESSION OF HEPATITIS C VIRUS CORE PROTEIN IN HUMAN MONOCYTE-DERIVED DENDRITIC CELLS USING RECOMBINANT SEMLIKI FOREST VIRUS Acta biol.Colomb. NAVAS,Maria-Cristina; STOLL-KELLER,Françoise; PAVLOVIC,Jovan. ABSTRACT Hepatitis C Virus belongs to the Flaviviridae family. One proposed mechanism of HCV persistence in the ability to infect hematopoietic cells, including Dendritic cells (DCs). HCV infection of DCs could impair their functions that represent one of the mechanisms, thus hampering viral clearance by the host immune system. Among HCV-encoded proteins, the highly conserved Core protein has been suggested to be responsible for the immunomodulatory properties of this Hepacivirus. Recombinant viral vectors expressing the HCV Core protein and allowing its transduction and therefore the expression of the protein into DCs could be useful tools for the analysis of the properties of the Core protein. Vaccinia Virus and retrovirus have been used to transduce... Tipo: Info:eu-repo/semantics/article Palavras-chave: Dendritic cells; HCV; SFV; Viral vector. Ano: 2019 URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2019000300493 Semliki forest virus as a vector: pros and cons for its use in biopharmaceuticals production BABT Núñez,Eutimio Gustavo Fernández; Jorge,Soraia Attie Calil; Astray,Renato Mancini; Rezende,Alexandre Gonçalves de; Costa,Bruno Labate Vale da; Monteiro,Daniella Cristina Ventini; Pereira,Carlos Augusto; Tonso,Aldo. The number of biopharmaceuticals for medical and veterinarian use produced in mammalian cells is increasing year after year. All of them are obtained by stable recombinant cell lines. However, it is recognized that transient gene expression produces high level expression in a short time. In that sense, viral vectors have been extensively used for producing recombinant proteins on lab-scale. Among them, Semliki Forest virus is commonly employed for this purpose. This review discusses the main aspects related to the use of Semliki Forest virus technology as well as its advantages and drawbacks which limit currently its utilization in biopharmaceutical industry on large-scale. Tipo: Info:eu-repo/semantics/article Palavras-chave: BHK-21; Large-scale bioprocesses; Mammalian cells; Recombinant proteins; SFV; Transient expression. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500018 Registros recuperados: 2 Primeira ... 1 ... Última

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