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Registros recuperados: 36 | |
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Okamoto,AS; Andreatti Filho,RL; Rocha,TS; Menconi,A; Marietto-Gonçalves,GA. |
The expansion of global poultry production has increased the need to reduce or control the agents responsible for economic losses, including Salmonella spp. These bacteria are also of public health concern due to their potential to cause food poisoning, and, more recently, due to the antimicrobial resistance presented by these bacteria. Molecular biology is an important tool currently used in the diagnosis and research studies of main poultry diseases. The present studied analyzed 100 samples of Salmonella Enteritidis (SE) isolated from avian material aiming at detecting the class 1 integron gene, Integroninvolved in antimicrobial resistance, by means of polymerase chain reaction (PCR), and comparing it with plate inhibition test. Subsequently, SE samples... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Integron; Gene transfer; Resistance to antimicrobials; Salmonella Enteritidis. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2009000300009 |
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Robeson,J; Retamales,J; Borie,C. |
Salmonella enterica serovar Enteritidis (SE) is a prevalent gastrointestinal pathogen worldwide, threatening both animal and human health. In the latter, disease is associated to the consumption of SE-contaminated products from the poultry industry. The control of SE infection is largely based on the use of antibiotics and vaccines, but the use of lytic bacteriophages is re-emerging as an additional strategy for SE control. In fact, a number of recent reports point to the adequacy of bacteriophage as an efficient prophylactic or therapeutic countermeasure to SE infections. However, less attention has been focused on the basic biology of these bacteriophages. Here we report on three bacteriophages (f18, IF1 and EST2) that share a common viral particle... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bacteriophages; Biocontrol; Salmonella Enteritidis. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2008000300007 |
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Coelho,LR; Melo,RT; Monteiro,GP; Reischak,D; Mendonça,AO; Tavares,AAS; Rossi,DA. |
ABSTRACT The analysis of Salmonella in the feces and the birds’ environment is a way of monitoring the colonization in the flocks and verifying the need for the introduction of stricter controls, in such a way that the results of the tests should be known before being sent for slaughter. The polymerase chain reaction (PCR), as well as other rapid methods represent alternatives increasingly used to detect enteric pathogens, but they need proof of effectiveness for their wide use. The aim of this study was to evaluate the equivalence between the results obtained by the methods: real-time PCR (BAX® System), Modified Rappaport-Vassiliadis Semi-solid Medium (MSRV) (ISO 6579) and the traditional method of official reference in Brazil for research of S.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BAX® System; MSRV; Salmonella Enteritidis; S. Typhimurium; Molecular biology. |
Ano: 2021 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2021000200303 |
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Albuquerque,ÁH; Cardoso,WM; Teixeira,RSC; Lopes,ES; Sales,RJPF; Horn,RV; Rocha-e-Silva,RC; Bezerra,WGA; Gomes-Filho,VJR. |
Two groups of domestic pigeons (Columba livia) were experimentally infected orally with doses of 9.5 x10(7) and 9.5 x10(9) CFU/mL (group A and B, respectively) of a Salmonella Enteritidis (SE) strain isolated from chickens. None of the used doses caused mortality of the inoculated birds; however, the pathogen was successfully recovered from the liver and spleen of group B birds on day 7 post-inoculation (dpi). Pathogen shedding, as evaluated through cloacal swabs, occurred in both groups until the 14th day of observation (p <0.05). Among all fecal samples collected from group B (n=4), three different birds shed the pathogen in their feces, out of which two were positive on 3 dpi and one on 7 dpi. The same number of fecal samples was evaluated in group A... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inoculation; Pigeons; Salmonella Enteritidis. |
Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2013000300007 |
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Li,X; Liu,L; Li,Q; Xu,G; Zheng,J. |
ABSTRACT Salmonella enterica subspecies enterica serotype Enteritidis (SE) has caused foodborne infections over decades. It is transmitted mainly from contaminated eggs to humans. SE is commonly present in layer houses, and closely interacts with environmental factors. The objective of the present study was to develop a viable PCR method to identify SE in environmental samples collected in layer farms of different sizes, and to evaluate SE contamination status in four main egg-production provinces of northern China. After specificity retrieval using Primer-BLAST against the NCBI database, three SE specific oligonucleotide primers were selected as candidate primers. The primers targeting Prot6e gene were adopted and primers targeting Sdf I were also... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Environment; Layer farm; PCR; Salmonella Enteritidis. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000300377 |
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Tayeb,IT; Nehme,P; Jaber,L; Barbour,EK. |
The present study aims at developing an indirect ELISA to quantify yolk antibodies specific to all surface proteins of the invasive Salmonella Enteritidis (SE), which acquired the 1.8, 14.1, and ~ 50 Kb plasmids. An ELISA checkerboard was used in four different experiments to account for the different parameters included in the preliminary ELISA procedure, and consequently to maximize the difference in Optical Density (OD) values between control positive and negative yolk samples. The first experiment aimed at studying the impact of 5% Bovine Serum Albumin (BSA) dissolved in distilled water as a blocking reagent on a 28 µg/well SE antigen-coated plate, while applying the positive and negative control yolk samples to different concentrations of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antibodies; Egg yolk; ELISA; Salmonella Enteritidis. |
Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2006000400009 |
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Cetin,B; Uran,H; Konak,M. |
ABSTRACT In this study, the effect of evaporated ethyl pyruvate (EP) was evaluated for the decontamination of Salmonella Enteritidis on chicken leg meat as a safe alternative to antimicrobial agent. Also, total aerobic mesophilic bacteria (TAMB), Enterobacteriaceae, Escherichia coli and yeast-mold counts samples were investigated. Subsequently, the samples were injected with 0, 42, 105 and 420 mg evaporated EP/L air to the paper filter attached to the container cover and stored at +4 °C for 10 days. According to the results, 42 mg EP/L concentration did not cause a significant decrease in Salmonella Enteritidis count (p>0.05). However, it was determined that 105 and 420 mg EP/L treatments reduced the number of Salmonella Enteritidis by more than 1 and 2... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chicken Meat; Decontamination; Ethyl Pyruvate; Salmonella Enteritidis. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000200301 |
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Milbradt,EL; Okamoto,AS; Padovani,CR; Fascina,VB; Silva,TM; Altarúgio,R; Hataka,A; Schmidt,EMS; Andreatti Filho,RL. |
ABSTRACT It is estimated that Salmonella causes one million illnesses in the United States annually, with 19,000 hospitalizations and 380 deaths. There are various serotypes of this bacterium, and the serotypes Typhimurium and Enteritidis are commonly isolated from outbreaks and, in most cases, present resistance to the antibiotics utilized in clinical medicine. One of the current theories to explain the emergence of bacterial resistance is the continuous use of antibiotics as growth promoters in animal feeds. Despite the lack of evidences that antibiotic growth promoters cause bacterial resistance, the poultry industry has explored strategies to reduce or to eliminate the use of antibiotic growth promoters in the production chain, including the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Commercial turkey; Competitive exclusion; Organic acid; Salmonella Enteritidis. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2017000400551 |
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Wu,Q; Liu,Z; Li,S; Jiao,C; Wang,Y; Wang,Y. |
ABSTRACT The aim was to investigate the effect of glutamine (Gln) on broilers challenged with Salmonella Enteritidis. 240 1-day-old birds were divided into four groups in a completely randomized design, each of which included 6 replicates with 10 birds per replicate. Group I served as the unchallenged, untreated control (CON). All birds in groups II (SCC) - IV were challenged with 2.0 × 104 CFU/mL of S. Enteritidis. Birds in group III and IV were treated with 0.5% (Gln 1) and 1.0% (Gln 2), respectively, of Gln. The results indicated that S. Enteritidis infection led to a decrease in the average body weight at d 7, 14, and 21 (p<0.05). Chickens fed the Gln showed improved average body weights in comparison with the SCC group (p<0.05). At d 4, 7, 14,... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glutamine; Average body weight; Antioxidant function; Salmonella Enteritidis; Broiler. |
Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2019000400307 |
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Silva,EN; Duarte,A. |
Salmonella Enteritidis (SE) emergiu como um grande problema avícola e de saúde pública no Brasil a partir de 1993. Os estudos epidemiológicos, incluindo a fagotipagem e sonda complementar de rRNA, sugerem a entrada de SE no Brasil via importação de material genético avícola contaminado, provavelmente no final da década de 80. As taxas de crescimento da avicultura brasileira na década de 90 criaram condições favoráveis para a manutenção e proliferação da SE nos plantéis avícolas. Além disso, o uso indiscriminado de antibióticos em aves, particularmente as quinolonas, encorajou a manutenção de lotes positivos para SE. As cepas de SE isoladas de aves têm mostrado alta sensibilidade aos antibióticos de uso comum em avicultura, incluindo as quinolonas.... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aves; Galinhas; Ovos; Salmonelose aviária; Salmonella Enteritidis. |
Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2002000200001 |
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Liu,Z; Wu,Q; Jiao,C; Cheng,B; Zhu,D; Ma,Y; Li,Y; Li,W. |
ABSTRACT The aim of this study was to investigate the effects of glutamine (Gln) on the intestinal mucosal structure and immune cells of broilers infected with Salmonella Enteritidis. 160 1-d-old commercial Arbor Acres (AA) broilers were randomly selected to receive one of four treatments, each of which had 5 replicates. Each replicate consisted of 8 chicks subjected to a 21-d feeding trial. Group I served as the unchallenged (CON). All birds in groups II (SCC) - IV were challenged with 2.0 × 104 CFU/mL of S. Enteritidis. The birds in groups III and IV were treated with 0.5% and 1.0% Gln. The results showed that S. Enteritidis infection led to a decrease in the relative length and weight, villus height:crypt depth (VH:CD) of the jejunum and ileum, the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glutamine; Intestinal villi; Mucosal immunity; Salmonella Enteritidis; Broiler. |
Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2020000300309 |
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Guastalli,BHL; Batista,DFA; Souza,AIS; Guastalli,EAL; Lopes,PD; Almeida,AM; Prette,N; Barbosa,FO; Stipp,DT; Freitas Neto,OC. |
ABSTRACT In poultry processing plants, disinfectants are often added to pre-chilling water tanks to reduce microbial contamination. The present study aimed at evaluating the effect of five disinfectants (acidified sodium chlorite, alkyl dimethyl benzyl ammonium chloride, chlorine dioxide, peracetic acid, and sodium hypochlorite) on the populations of food quality indicator microorganisms and on Salmonella Enteritidis (SE) in the presence and absence of organic matter. The results showed that chlorine dioxide and sodium hypochlorite did not reduce microbial carcass counts. On the other hand, acidified sodium chlorite, alkyl dimethyl benzyl ammonium chloride and peracetic acid reduced total and fecal coliform counts. Peracetic acid reduced the number of... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Broiler carcasses; Disinfectant; Indicator microorganisms; Pre-chilling tank; Salmonella Enteritidis. |
Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200217 |
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MIRANDA,ANDREA L.; CORDEIRO,SORAIA M.; REIS,JOICE N.; CARDOSO,LUCAS G.; GUIMARÃES,ALAÍSE G.. |
ABSTRACT Increasing antimicrobial resistance in Salmonella species has been a serious problem for public health worldwide. This study examines Salmonella spp. recovered from foods and clinical samples on serotype, antimicrobial resistance and PFGE genotypes. It identified 91 salmonellae, belonging to 31 different serotypes, from 36 isolates from food and 55 clinical samples. Salmonella Infantis (16.5%) and Salmonella Enteritidis (13.7%) are the most common among food isolates, whereas Salmonella Enteritidis (29.0%) and Salmonella Typhimurium (16.0%) mainly causes human salmonellosis. Antimicrobial susceptibility data showed that 63.0% of the isolates were fully susceptible to 12 antibiotics tested. Nalidixic acid showed high resistance rates, 32.7% and... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Salmonella; Salmonella Infantis; Salmonella Enteritidis; Antimicrobial resistance. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652017000301143 |
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Ribeiro,A.R.; Kellermann,A.; Santos,L.R.; Nascimento,V.P.. |
The antimicrobial resistance of Salmonella Enteritidis strains isolated from clinical and environmental poultry samples in the Southern Brazil during the years of 1999, 2000 and 2001 was evaluated. Among the 79 isolated samples, 64 (81%) were resistant to at least one of the antimicrobial agents tested, showing 22 different resistance patterns. Tetracycline showed the highest percentage (64,5%) of resistance among the antimicrobial agents used. Resistance to drugs at different levels was found as the following: ampicillin (1.2%), kanamycin (1.2%), ciprofloxacin (2.5%), enrofloxacin (8.8%), gentamicin (21.5%), streptomycin (20.2%), nitrofurantoin (26.6%), and nalidixic acid (30.4%). None of the S. Enteritidis strains were resistant to chloramphenicol,... |
Tipo: Info:eu-repo/semantics/other |
Palavras-chave: Ave; Salmonella Enteritidis; Resistência antimicrobiana. |
Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000500032 |
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Registros recuperados: 36 | |
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