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| Viallon, Jerome; Chinain, Mireille; Darius, Helene Taiana. |
| The neuroblastoma cell-based assay (CBA-N2a) is widely used for the detection of marine biotoxins in seafood products, yet a consensus protocol is still lacking. In this study, six key parameters of CBA-N2a were revisited: cell seeding densities, cell layer viability after 26 h growth, MTT incubation time, Ouabain and Veratridine treatment and solvent and matrix effects. A step-by-step protocol was defined identifying five viability controls for the validation of CBA-N2a results. Specific detection of two voltage gated sodium channel activators, pacific ciguatoxin (P-CTX3C) and brevetoxin (PbTx3) and two inhibitors, saxitoxin (STX) and decarbamoylsaxitoxin (dc-STX) was achieved, with EC(50)values of 1.7 +/- 0.35 pg/mL, 5.8 +/- 0.9 ng/mL, 3 +/- 0.5 ng/mL... |
| Tipo: Text |
Palavras-chave: CBA-N2a; Standardization; Matrix effects; Absorbance data; Ciguatoxins; Brevetoxins; Saxitoxins; Biological sample; Seafood safety. |
| Ano: 2020 |
URL: https://archimer.ifremer.fr/doc/00656/76859/78083.pdf |
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| Geffroy, Solene; Lechat, Marc-marie; Le Gac, Mickael; Rovillon, Georges-augustin; Marie, Dominique; Bigeard, Estelle; Malo, Florent; Amzil, Zouher; Guillou, Laure; Caruana, Amandine. |
| Paralytic shellfish poisoning (PSP) is a human foodborne syndrome caused by the consumption of shellfish that accumulate paralytic shellfish toxins (PSTs, saxitoxin group). In PST-producing dinoflagellates such as Alexandrium spp., toxin synthesis is encoded in the nuclear genome via a gene cluster (sxt). Toxin production is supposedly associated with the presence of a 4th domain in the sxtA gene (sxtA4), one of the core genes of the PST gene cluster. It is postulated that gene expression in dinoflagellates is partially constitutive, with both transcriptional and post-transcriptional processes potentially co-occurring. Therefore, gene structure and expression mode are two important features to explore in order to fully understand toxin production processes... |
| Tipo: Text |
Palavras-chave: Alexandrium; Saxitoxins; SxtA4; Copy number variation; SxtA; Expression; Isoform. |
| Ano: 2021 |
URL: https://archimer.ifremer.fr/doc/00682/79402/81944.pdf |
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