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	Provedor de dados Electron. J. Biotechnol. (3) BABT (1) BJMBR (1) Autor Bell,Antonio (1) Carrera,Emilia (1) Castilho,L.R. (1) Fernandes,A.M. (1) Gopalaraman,Swaminathan (1) Jaisil,Prasit (1) Kumar Kukreja,Arun (1) Laopaiboon,Lakkana (1) Laopaiboon,Pattana (1) Mahadevan,Surianarayanan (1) Mariante,R.M. (1) Marinho,P.A.N. (1) Martínez,Aidín (1) Mehrotra,Shakti (1) Michelena,Georgina L. (1) Nath Mishra,Bhartendu (1) Paulsen,B.S. (1) Rehen,S.K. (1) Sartore,R.C. (1) Singh Khanuja,Suman Preet (1) Mais... Palavra-chave Scale-up (5) Agrobacterium (1) Biocalorimeter (1) Bioethanol (1) Bioreactor (1) Dextransucrase enzyme (1) Dried spent yeast (1) Fed batch fermentation (1) Glycyrrhiza (1) Human embryonic stem cells (1) Leuconostoc mesenteroides (1) Metabolic heat (1) Microcarriers (1) Oxygen transfer (1) P. aeruginosa (1) Spinner bioreactors (1) Sweet sorghum juice (1) Transformed roots (1) Very high gravity fermentation (1) Volumetric mass transfer coefficient (1) Mais... Tipo do documento Journal article (3) Info:eu-repo/semantics/article (2) Ano 2011 (1) 2009 (1) 2008 (2) 2003 (1) País Chile (3) Brazil (2) Idioma Inglês (5)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 5 Primeira ... 1 ... Última The use of dried spent yeast as a low-cost nitrogen supplement in ethanol fermentation from sweet sorghum juice under very high gravity conditions Electron. J. Biotechnol. Sridee,Worawut; Laopaiboon,Lakkana; Jaisil,Prasit; Laopaiboon,Pattana. Dried spent yeast (DSY) was used as a low-cost nitrogen supplement for ethanol fermentation from sweet sorghum juice under very high gravity (VHG) conditions by Saccharomyces cerevisiae NP 01. The fermentation was carried out at 30ºC in a 5-litre bioreactor. The results showed that DSY promoted ethanol production efficiencies. The ethanol concentration (P), productivity (Qp) and yield (Yp/s) of the sterile juice (total sugar of 280 g l-1) supplemented with 8 g l-1 of DSY were not different from those supplemented with yeast extract and/or peptone at the same amount. The initial yeast cell concentration of 5 x 10(7) cells ml-1 was found to be optimal for scale-up ethanol production. In addition, an increase in sugar concentration in inoculum preparation... Tipo: Journal article Palavras-chave: Bioethanol; Dried spent yeast; Scale-up; Sweet sorghum juice; Very high gravity fermentation. Ano: 2011 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000600003 Genetic transformation studies and scale up of hairy root culture of Glycyrrhiza glabra in bioreactor Electron. J. Biotechnol. Mehrotra,Shakti; Kumar Kukreja,Arun; Singh Khanuja,Suman Preet; Nath Mishra,Bhartendu. The study was undertaken to induce hairy roots in Glycyrrhiza glabra in leaf explants and to optimize the nutritional requirement for its growth kinetics at shake flask and bioreactor level. Pathogenecity of Agrobacterium depends upon transformation ability of strain and age, type, and physiological state of explants. Agrobacterium rhizogenes strain K599 was used to infect leaf explants of G. glabra. Explants of different age groups were obtained from 2 to 5 weeks old in vitro grown cultures. Bacterial strain K599 could induce hairy roots in 3 and 4 weeks old leaf explants cultured on B5, MS, NB and WP basal semi-solid medium. Leaf explants of 2 and 5 weeks old culture were not responsive to bacterial infection in terms of hairy root induction. Maximum... Tipo: Journal article Palavras-chave: Agrobacterium; Bioreactor; Glycyrrhiza; Scale-up; Transformed roots. Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000200009 Oxygen mass transfer studies on batch cultivation of P. aeruginosa in a biocalorimeter Electron. J. Biotechnol. Sivaprakasam,Senthilkumar; Mahadevan,Surianarayanan; Gopalaraman,Swaminathan. In the present work volumetric mass transfer coefficient (kLa) was investigated during batch cultivations of Pseudomonas aeruginosa on a nutrient media. The effects of process variables (viz. impeller speed, oxygen flow and geometry of impeller) on the volumetric mass transfer coefficient of oxygen, kLa, in a biocalorimeter (Bio-RC1) was investigated and reported in this research work. The experimental data have been analyzed employing MATLAB to obtain the influences of the process parameters on kLa. An attempt was made to correlate volumetric mass transfer coefficient with metabolic heat production rate at optimized process conditions. The correlation reported in this work would be useful to control and scale up of bioprocesses. Tipo: Journal article Palavras-chave: Biocalorimeter; Metabolic heat; P. aeruginosa; Scale-up; Volumetric mass transfer coefficient. Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100008 Scale-up of dextransucrase production by Leuconostoc mesenteroides in fed batch fermentation BABT Michelena,Georgina L.; Martínez,Aidín; Bell,Antonio; Carrera,Emilia; Valencia,Roxana. Fed batch fermentation was carried out for the dextransucrase enzyme production from Leuconostoc mesenteroides and the production was scale-up using oxygen transfer criteriuom. It was found that in 5 L vessel fermentation capacity, the best agitation speed was 225 min-1 and aeration rate was 0.15 vvm, obtaining dextransucrase activity of 127 DSU/mL.. The maximum enzyme production velocity coincide with the maximum growth velocity between 6 and 7 h of fermentation, which confirmed that dextransucrase production was associated with microbial growth. High enzyme yields were achieved during scale up based on oxygen transfer rate. Tipo: Info:eu-repo/semantics/article Palavras-chave: Leuconostoc mesenteroides; Dextransucrase enzyme; Scale-up; Oxygen transfer; Fed batch fermentation. Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000300017 Successful scale-up of human embryonic stem cell production in a stirred microcarrier culture system BJMBR Fernandes,A.M.; Marinho,P.A.N.; Sartore,R.C.; Paulsen,B.S.; Mariante,R.M.; Castilho,L.R.; Rehen,S.K.. Future clinical applications of human embryonic stem (hES) cells will require high-yield culture protocols. Currently, hES cells are mainly cultured in static tissue plates, which offer a limited surface and require repeated sub-culturing. Here we describe a stirred system with commercial dextran-based microcarriers coated with denatured collagen to scale-up hES cell production. Maintenance of pluripotency in the microcarrier-based stirred system was shown by immunocytochemical and flow cytometry analyses for pluripotency-associated markers. The formation of cavitated embryoid bodies expressing markers of endoderm, ectoderm and mesoderm was further evidence of maintenance of differentiation capability. Cell yield per volume of medium spent was more than... Tipo: Info:eu-repo/semantics/article Palavras-chave: Human embryonic stem cells; Scale-up; Microcarriers; Spinner bioreactors. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000600007 Registros recuperados: 5 Primeira ... 1 ... Última

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