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	Provedor de dados Genet. Mol. Biol. (2) Autor Arda,Nazli (1) Karaer,Semian (1) Sarikaya,Aysegul Topal (1) Sarikaya,Aysegül Topal (1) Temizkan,Güler (1) Uz,Gulsen (1) Mais... Palavra-chave Schizosaccharomyces pombe (2) Gua1 gene (1) Inosine monophosphate dehydrogenase (1) Magnesium (1) Microtubule (1) Mitosis (1) Mitotic spindle (1) Purine nucleotide pathway (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2016 (1) 2006 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última The 3' terminal sequence of the inosine monophosphate dehydrogenase gene encodes an active domain in the yeast Schizosaccharomyces pombe Genet. Mol. Biol. Karaer,Semian; Sarikaya,Aysegül Topal; Arda,Nazli; Temizkan,Güler. The gua1 gene encoding inosine monophosphate dehydrogenase (IMPDH), which catalyses the first step in de novo biosynthesis of guanosine monophosphate (GMP), was cloned in the yeast Schizosaccharomyces pombe by functional complementation of a gua1ura4-D18 mutant strain from a S. pombe DNA genomic library. Complementation analysis revealed a 1.2 kb fragment which segregation analysis confirmed did not code for a suppressor gene. Only 446 nucleotides of the gua1 gene encoding the IMPDH C-terminal residues were found within this 1.2 kb sequence (GenBank, AJ293460). The comparison of this wild-type fragment with the same fragment from the gua1ura4-D18 mutant revealed that there was a point mutation at position 1261 (guanine -> adenine) from the 5' end,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Schizosaccharomyces pombe; Inosine monophosphate dehydrogenase; Gua1 gene; Purine nucleotide pathway. Ano: 2006 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300026 The effect of magnesium on mitotic spindle formation in Schizosaccharomyces pombe Genet. Mol. Biol. Uz,Gulsen; Sarikaya,Aysegul Topal. Abstract Magnesium (Mg2+), an essential ion for cells and biological systems, is involved in a variety of cellular processes, including the formation and breakdown of microtubules. The results of a previous investigation suggested that as cells grow the intracellular Mg2+ concentration falls, thereby stimulating formation of the mitotic spindle. In the present work, we used a Mg2+-deficient Schizosaccharomyces pombe strain GA2, in which two essential membrane Mg2+ transporter genes (homologs of ALR1 and ALR2 in Saccharomyces cerevisae) were deleted, and its parental strain Sp292, to examine the extent to which low Mg2+ concentrations can affect mitotic spindle formation. The two S. pombe strains were transformed with a plasmid carrying a GFP-α2-tubulin... Tipo: Info:eu-repo/semantics/article Palavras-chave: Magnesium; Microtubule; Mitosis; Mitotic spindle; Schizosaccharomyces pombe. Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300459 Registros recuperados: 2 Primeira ... 1 ... Última

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