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| Moraes,C.M.; Conceição,F.R.; Rocha,A.S.R.; Santos Júnior,A.G.; Ribas,L.M.; Vargas,A.P.C.; Nogueira,C.E.W.; Gil-Turnes,C.; Leite,F.P.L.. |
| Strangles is an economically important horse disease caused by Streptococcus equi subsp. equi. The diagnosis can be confirmed either directly by bacterial isolation and PCR or by ELISA, which is an indirect method based on the detection of serum antibodies. The aim of this study was to clone, express and characterize the SeM protein of Streptococcus equi subsp. equi, evaluate its use as antigen in indirect ELISA and determine its performance to distinguish sera of negative, vaccinated and positive animals. This was initially performed by cloning the gene encoding the SeM protein and its expression in Escherichia coli. Subsequently, the protein produced was characterized and used as antigen in ELISA. Serum samples for evaluation were taken from 40 negative... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Strangles Streptococcus equi; SeM ELISA. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000401015 |
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