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Registros recuperados: 56
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Diversidad y caracterización de poblaciones bacterianas asociadas a esclerocios de Claviceps gigantea Fuentes et al. en maíz (Zea mays L.) con potencial antagonista, en el Estado de México. Colegio de Postgraduados
Ayala Torres, Ana María.
Un total de 129 aislamientos bacterianos obtenidos de la micósfera de esclerocios de Claviceps gigantea se evaluaron in vitro para determinar su capacidad de inhibir el crecimiento de Fusarium graminearum, F. subglutinans y F. verticillioides patógenos a maíz. Ensayos cualitativos en confrontación dual, resultaron en la selección de 22 aislamientos bacterianos con el mayor potencial antagónico hacia una, dos o las tres especies de Fusarium. Los aislamientos se identificaron molecularmente mediante secuenciación parcial del gen 16s ADNr. Las cepas con mayor grado de antagonismo pertenecen a los géneros Bacillus y Pseudomonas. La caracterización en la producción de metabolitos potencialmente involucrados en la actividad antagónica, mostró que el 100% de las...
Palavras-chave: Bacillus; Pseudomonas; Biocontrol; Metabolitos secundarios; Caracterización; Secondary metabolites; Characterization; Fitopatología; Maestría.
Ano: 2014 URL: http://hdl.handle.net/10521/2299
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Identificación de dos spp de Ipomoea y evaluación in vitro del extracto metanólico como problema de intoxicación en cabras. Colegio de Postgraduados
Mila Arango, Roberta.
El desconocimiento de distintas plantas tóxicas y la ingesta de éstas por rebaños caprinos en temporada de sequía, ha provocado que muchos casos de intoxicación no se diagnostiquen o sean confundidos con otras causas, provocando altos índices de morbilidad y mortalidad en rebaños caprinos. El presente estudio tuvo como objetivo principal, la identificación de los metabolitos secudarios presentes en dos especies arbustivas de la Mixteca baja de Oaxaca causantes de intoxicación en cabras. Se evaluó el grado de toxicidad de estas plantas mediante un bioensayo con crustáceos, mismo que sirvió para identificar los metabolitos secundarios más perjudiciales y determinar, mediante la técnica de gas in vitro, el grado de afección en el rumen. Se evaluó un agente...
Palavras-chave: Caprinos; I. murucoides; I. pauciflora; Metabolitos secundarios; Toxicidad; Goats; Secondary metabolites; Toxicity; Ganadería; Maestría.
Ano: 2013 URL: http://hdl.handle.net/10521/1905
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Micorriza arbuscular y estrés abiótico en el contenido de alcaloides (vinblastima y vincristina) de Catharanthus roseus (L.) G. Don Colegio de Postgraduados
Rosa Mera, Claudia Janette de la.
Este estudio evaluó el incremento de vinblastina y vincristina en plantas micorrizadas de Catharanthus roseus (L.) G. Don., establecidas en invernadero, sometidas a estrés abiótico causado por aplicaciones de bicarbonato de potasio (2.5 y 7.5 mM) y cloruro de sodio (40 mM). El presente trabajo fue dividido en tres fases experimentales. La primera fase describe la propagación de consorcios e identificación de hongos micorrízicos arbusculares (HMA) obtenidos de la rizósfera de plantas medicinales recolectadas del Parque Nacional Iztapopo, Zoquiapan y Anexas. En la segunda fase se realizó una selección de consorcios de HMA con base en la promoción del crecimiento en C. roseus. Finalmente, en la tercera fase se realizó una aplicación de bicarbonato de...
Tipo: Tesis Palavras-chave: Planta medicinal; Vinca; Metabolitos secundarios; Alcalinidad; Salinidad; Maestría; Edafología; Medicinal plants; Secondary metabolites; Alkalinity; Salinity.
Ano: 2009 URL: http://hdl.handle.net/10521/1303
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Novel antimicrobial secondary metabolites from a Penicillium sp. isolated from Brazilian cerrado soil Electron. J. Biotechnol.
Petit,Philippe; Lucas,Esther M. F.; Abreu,Lucas M; Pfenning,Ludwig H; Takahashi,Jacqueline A.
The morphological features of a Penicillium, isolated from Brazilian cerrado soil, were characterized and showed to be distinctly different from all well-defined Penicillium species. Chemical and biological investigation on the ethyl acetate extract of this Penicillium isolate resulted in the isolation of three new naphthalenoids: a major metabolite, methyl 6-acetyl-4-methoxy-5,7,8-trihydroxynaphthalene-2-carboxylate and two minor ones, methyl 6-acetyl-4-methoxy-7,8-dihydroxynaphthalene-2-carboxylate and methyl 6-acetyl-4-methoxy-5,8-dihydroxynaphthalene-2-carboxylate. Their structures were determined based on their mono and bidimensional nuclear magnetic resonance data. Acetyl, allyl and methoxyl derivatives of the major metabolite were prepared in order...
Tipo: Journal article Palavras-chave: Antibacterial activity; Cerrado; Naphthalenoids; Penicillium; Secondary metabolites.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400008
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Isolation of functional total RNA from Argemone mexicana tissues Electron. J. Biotechnol.
Rubio-Piña,Jorge A; Vázquez-Flota,Felipe A.
RNA extraction from recalcitrant plant tissues is frequently complicated by the presence of secondary metabolites, polysaccharides and polyphenols. These compounds may co precipitate with RNA, often rendering it unsuitable for either cDNA synthesis or hybridization in northern blot analyses and therefore, interfering with the gene analysis expression in such tissues. We have developed an efficient RNA extraction method from A. mexicana tissues. The procedure includes the use of polyvinylpolypyrrolidone (PVPP), to remove secondary metabolites, proteins and polyphenols, and a two-step precipitation with LiCl, to eliminate polysaccharides, and thus increasing RNA yield. The quality of the resulting RNA was evaluated spectrophotometrically and by agarose gel...
Tipo: Journal article Palavras-chave: Argemone mexicana; Plant tissues; RNA isolation; Secondary metabolites.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400015
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Fast and reliable calibration of solid substrate fermentation kinetic models using advanced non-linear programming techniques Electron. J. Biotechnol.
Araya,M. Macarena; Arrieta,Juan J; Pérez-Correa,J. Ricardo; Biegler,Lorenz T; Jorquera,Héctor.
Calibration of mechanistic kinetic models describing microorganism growth and secondary metabolite production on solid substrates is difficult due to model complexity given the sheer number of parameters needing to be estimated and violation of standard conditions of numerical regularity. We show how advanced non-linear programming techniques can be applied to achieve fast and reliable calibration of a complex kinetic model describing growth of Gibberella fujikuroi and production of gibberellic acid on an inert solid support in glass columns. Experimental culture data was obtained under different temperature and water activity conditions. Model differential equations were discretized using orthogonal collocations on finite elements while model calibration...
Tipo: Journal article Palavras-chave: Dynamic models; Gibberellic acid; Nonlinear models; Parameter estimation; Secondary metabolites; Solid substrate cultivation.
Ano: 2007 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100005
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BIOPROSPECTING: A TOOL TO CONSERVE CHILEAN BRYOPHYTES Gayana Botánica
Cuvertino-Santoni,Jorge; Montenegro,Gloria.
Here, we present the current state of knowledge on the diversity of Chilean bryophytes (mosses, liverworts, and hornworts), the secondary metabolites present in these plants, and the biological activity of extracts from populations of Chilean species. Our goal is to establish the potential of these plants as a source of useful molecules for pharmaceutical and agricultural industries, thus promoting their conservation. Only 3,7% of Chilean bryophytes (55 spp.) have been analyzed using chemical characterization of their extracts (methanol, ether, ethanol, dichloromethane, acetone and hexane). Only four species from Chilean and Argentinian populations (Porella chilensis (Lehm. & Lindenb.) Trevi., Riccardiapolyclada (Mitt. ex Thurn) Hässel,...
Tipo: Journal article Palavras-chave: Mosses; Liverworts; Hornworts; Uses; Secondary metabolites; Biological activity; In vitro cultivation.
Ano: 2013 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-66432013000100002
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Ammonium/nitrate ratio affects the growth and glucosinolates content of pakchoi Horticultura Brasileira
Yang,Jing; Zhu,Biao; Ni,Xiaolei; He,Yong.
ABSTRACT Nitrogen (N) strongly affects plant growth. However, little is known about the effects of the ammonium/nitrate ratio on pakchoi (Brassica rapa), especially its glucosinolates (GSs) contents which are involved in plant defense and many of them benefit to human health. The aim of this study was to evaluate the effects of a constant N supply (8 mM) but with five ammonium/nitrate ratios (namely 0/8 mM, 2/6 mM, 4/4 mM, 6/2 mM and 8/0 mM) on the growth of pakchoi in a hydroponic system in 2 years. In both years, a higher biomass (dry weight) was in the 4/4 and 2/6 ammonium/nitrate treatments (2.3 and 2.2-fold compared to 8/0, respectively), with no significant difference in biomass between these two treatments. The biomass then decreased with increasing...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Brassica rapa; Biomass; Nitrogen form; Secondary metabolites.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362020000300246
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Cross talk: Two way allelopathic interactions between toxic Microcystis and Daphnia ArchiMer
Bojadzija Savic, Gorenka; Bormans, Myriam; Edwards, Christine; Lawton, Linda; Briand, Enora; Wiegand, Claudia.
Due to eutrophication, freshwater ecosystems frequently experience cyanobacterial blooms, many of which produce bioactive metabolites that can affect vertebrates and invertebrates life traits. Zooplankton are able to develop tolerance as a physiological response to cyanobacteria and their bioactive compounds, however, this comes with energetic cost that in turn influence Daphnia life traits and may impair populations. Vice versa, it has been suggested that Daphnia are able to reduce cyanobacterial dominance until a certain cyanobacterial density; it remains unclear whether Daphnia metabolites alone influence the physiological state and bioactive metabolites production of cyanobacteria. Hence, this study investigates mutual physiological reactions of toxic...
Tipo: Text Palavras-chave: Zooplankton; Cyanobacteria; Secondary metabolites; PCC7806; Toxic; Oxidative stress.
Ano: 2020 URL: https://archimer.ifremer.fr/doc/00624/73653/76105.pdf
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Daphnia magna Exudates Impact Physiological and Metabolic Changes in Microcystis aeruginosa ArchiMer
Bojadzija Savic, Gorenka; Edwards, Christine; Briand, Enora; Lawton, Linda; Wiegand, Claudia; Bormans, Myriam.
While the intracellular function of many toxic and bioactive cyanobacterial metabolites is not yet known, microcystins have been suggested to have a protective role in the cyanobacterial metabolism, giving advantage to toxic over nontoxic strains under stress conditions. The zooplankton grazer Daphnia reduce cyanobacterial dominance until a certain density, which may be supported by Daphnia exudates, affecting the cyanobacterial physiological state and metabolites’ production. Therefore, we hypothesized that D. magna spent medium will impact the production of cyanobacterial bioactive metabolites and affect cyanobacterial photosynthetic activity in the nontoxic, but not the toxic strain. Microcystin (MC-LR and des-MC-LR) producing M. aeruginosa PCC7806 and...
Tipo: Text Palavras-chave: Cyanobacteria; Secondary metabolites; PCC7806; Toxic; Mutant; Infochemicals.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00507/61893/65980.pdf
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Causative Agents of Aspergillosis Including Cryptic Aspergillus Species and A. fumigatus OAK
豊留, 孝仁; TOYOTOME, Takahito.
アスペルギルス症は深在性真菌感染症の重要な位置を占めている.アスペルギルス症の原因真菌はAspergillus fumigatusが最も重要であるが,そのほかにもAspergillus flavusやAspergillus niger, Aspergillus terreusなども重要な原因菌種である.また,近年になって隠蔽種と呼ばれる各Aspergillus sectionの代表的な種に非常によく似た菌種の存在が認識され,一部のアスペルギルス症の原因となっていることも分かってきた.最近の研究で,それらの菌種間では抗真菌薬に対する感受性や二次代謝産物の産生プロファイル (エクソメタボローム) が異なることも明らかとなってきた.一方,治療中のA. fumigatusアゾール耐性化や近年の欧州を中心とした環境中アゾール耐性A. fumigatusの出現も報告され,これら菌種を正確に同定し,加えて感受性を評価することの重要性が高まってきている. 本総説ではA. fumigatusを含めたアスペルギルス症原因菌種,特に隠蔽種について触れ,エクソメタボローム解析を利用した同定の可能性を述べる.加えて,A. fumigatusアゾール系抗真菌薬耐性について最近の報告を交えて概説したい
Palavras-chave: Aspergillosis; Aspergillus spp.; Azole resistance; Cryptic species; Secondary metabolites.
Ano: 2016 URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4396
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Influência do óleo essencial na micorrização e no crescimento de mudas de eucalipto PFB - Pesquisa Florestal Brasileira
Steffen, Ricardo Bemfica; Antoniolli, Zaida Inês; Steffen, Gerusa Pauli Kist; Jacques, Rodrigo Josemar Seminoti; Echkardt, Daniel Pazzini; dos Santos, Marcos Leandro; Santana, Natielo Almeida.
O estabelecimento das essências florestais exóticas pode apresentar dependência da associação ectomicorrízica, a qual  eleva a resistência das mudas aos estresses iniciais após o  plantio no campo, favorecendo o desenvolvimento inicial das mudas sob condições adversas. O trabalho objetivou avaliar o  efeito da aplicação do óleo essencial de eucalipto na  colonização ectomicorrízica e no crescimento de mudas de  Eucalyptus grandis, em condições de casa de vegetação. Os tratamentos foram constituídos por cinco concentrações do óleo  essencial de Eucayptus grandis e dois isolados  ectomicorrízicos, além dos tratamentos controle sem  inoculação. Utilizou-se delineamento experimental inteiramente casualizado com oito repetições. Noventa dias após o  transplante...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ectomicorrizas; Simbiose; Metabólitos secundárioslitos secundários Ectomycorrhizae; Symbiosis; Secondary metabolites.
Ano: 2011 URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/260
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Eucalyptus essential oil action on mycorrhizal colonization and the establishment of Eucalyptus grandis in soil contaminated by copper PFB - Pesquisa Florestal Brasileira
Steffen, Ricardo Bemfica; Antoniolli, Zaida Inês; Steffen, Gerusa Pauli Kist; Jacques, Rodrigo Josemar Seminoti; da Silva, Rodrigo Ferreira.
The use of eucalyptus essential oil can optimize the growth of ectomycorrhizal isolates, which aid the establishment of forest species in soils contaminated by copper. The study aimed to determine the best application of eucalyptus essential oil in the formation of mycorrhizal seedlings of Eucalyptus grandis and its influence on the establishment of these seedlings in soil contaminated by copper. It was used the ectomycorrhizal fungi (fECM) Pisolithus microcarpus. The application forms of the essential oil were evaluated in a greenhouse, by means of six treatments. Subsequently, the seedlings were transplanted and evaluated in soil contaminated by copper, greenhouse and field. It was determined height, diameter, fresh and dry weight of shoots and roots,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Heavy metals; Secondary metabolites; Ectomycorrhizal Metais pesados; Metabólitos secundários; Fungos ectomicorrízicos.
Ano: 2011 URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/268
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Efeito do óleo essencial de Eucalyptus grandis no crescimento de isolados de fungos ectomicorrízicos em diferentes concentrações de cobre, zinco e níquel PFB - Pesquisa Florestal Brasileira
Steffen, Ricardo Bemfica; Antoniolli, Zaida Inês; Steffen, Gerusa Pauli Kist; Jacques, Rodrigo Josemar Seminoti; Echkardt, Daniel Pazzini; dos Santos, Marcos Leandro; Santana, Natielo Almeida.
Os metabólitos secundários bioativos de algumas plantas são capazes de estimular o crescimento dos fungos ectomicorrízicos. Quando em associação com plantas, esses fungos proporcionam as mesmas condições de se desenvolverem em ambientes contaminados por metais pesados. Avaliou-se o efeito da adição de óleo essencial de Eucalyptus grandis no crescimento de isolados ectomicorrízicos na presença de cobre, zinco e níquel. Os fungos ectomicorrízicos Pisolithus microcarpus (UFSC Pt 116) e Pisolithus sp. (UFSC Pt 24) foram incubados em meio de cultura líquido na presença de concentrações crescentes de cobre, zinco e níquel e de óleo essencial de Eucalyptus grandis na concentração de 20 μL L-1. Após período de incubação de 25 dias, avaliou-se a massa seca do...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Metais pesados; Metabólitos secundários; Ectomicorrizas Heavy metals; Secondary metabolites; Ectomycorrhizae..
Ano: 2011 URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/259
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Plant compounds insecticide activity against Coleoptera pests of stored products PAB
Moreira,Márcio Dionizio; Picanço,Marcelo Coutinho; Barbosa,Luiz Cláudio de Almeida; Guedes,Raul Narciso Carvalho; Campos,Mateus Ribeiro de; Silva,Gerson Adriano; Martins,Júlio Cláudio.
The objective of this work was to screen plants with insecticide activity, in order to isolate, identify and assess the bioactivity of insecticide compounds present in these plants, against Coleoptera pests of stored products: Oryzaephilus surinamensis L. (Silvanidae), Rhyzopertha dominica F. (Bostrichidae) and Sitophilus zeamais Mots. (Curculionidae). The plant species used were: basil (Ocimum selloi Benth.), rue (Ruta graveolens L.), lion's ear (Leonotis nepetifolia (L.) R.Br.), jimson weed (Datura stramonium L.), baleeira herb (Cordia verbenacea L.), mint (Mentha piperita L.), wild balsam apple (Mormodica charantia L.), and billy goat weed or mentrasto (Ageratum conyzoides L.). The insecticide activity of hexane and ethanol extracts from those plants on...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Natural insecticide; Pest management; Alternative control; Secondary metabolites.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2007000700001
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Morpho-anatomical characters and secondary metabolites from Psittacanthus calyculatus (Loranthaceae) Phyton
Sánchez-Arreola,Eugenio; Maiti,RK; Trujillo-Pérez,Bertín.
Psittacanthus calyculatus is a parasitic plant. The presence of alkaloids, saponins and terpenes indicates its potential medicinal values, and antimicrobial activities.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Psittacanthus calyculatus; Morphology; Anatomy; Secondary metabolites.
Ano: 2004 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572004000100014
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Yield and Composition of the Essential oil of Tetradenia riparia (Hochst) Codd (Lamiaceae) Cultivated Under Different Shading Levels Planta Daninha
ARAÚJO,L.L.N.; MELO,H.C.; PAULA,J.R.; ALVES,F.R.R.; PORTES,T.A..
ABSTRACT: Light has direct influence on growth and development by altering the morphophysiology of the plant and the content and composition of secondary metabolites. The present study aimed to evaluate the yield and composition of the essential oil of Tetradenia riparia cultivated under different shading levels. Plants were propagated by cuttings and cultivated either under full sunlight and in environment completely covered by black polyethylene sheeting to obtain shading levels of 30%, 50%, and 80%. Plants were grown under experimental conditions for 150 days. The essential oil was extracted from fresh leaves in triplicate for each treatment through steam distillation with a modified Clevenger apparatus. The components of the essential oil were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 14-hydroxy-9-epi-(E)-caryophyllene; Medicinal plant; Secondary metabolites; Shading levels.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100266
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Activities and Novel Applications of Secondary Metabolite Coumarins Planta Daninha
HUSSAIN,M.I.; QAMAR ABBAS,S.; REIGOSA,M.J..
ABSTRACT: This review covers biological activity of secondary metabolites, coumarin and their derivatives on different organisms (pests, pathogens, weeds), as well as their applications in agriculture as eco-friendly pesticides and weed control agents. Naturally occurring coumarins have shown biological and allelopathic potential on a broad range of organisms. Coumarin inhibits seed germination, root growth, morphology, histology, water uptake, respiration, photosynthesis, cell elongation, cell división and differentiation. Coumarin demonstrate excellent insecticidal potential against eggs, larvae and adult insects. Coumarin showed antifeedent responses in a wide range of memebers from invertebrates to vertebrates. The information provided in this review...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coumarin; Secondary metabolites; Multifunctionality; Herbivory; Allelopathy; Phytotoxicity; Weed control.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582018000100220
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Antimicrobial Activity and Phytochemical Screening of Euphorbia helioscopia Planta Daninha
WAHEED,K.; MUHAMMAD,S.K.; SHOMAILA,A.; MUHAMMAD,Z.; IZHAR,U.; ULLAH,S..
ABSTRACT: Medicinal Plants have been used throughout the world by human beings as a drug and remedies for various diseases since time immemorial. A study was planned to count into the antimicrobial activity and phytochemical screening of Euphorbia helioscopia. The plants were gathered and tested against some standard strains and some human pathogenic microorganisms i.e Escherichia coli, Bacillus Subtilis, Staphylococcus aureus, Klebsiella pneumoniae, Salmonella typhi, Pseudomonas aeruginosa and three fungal strain Trichoderma, R hizopus nigricans, Aspergillus niger. The concentrations of extracting samples (500 and 1,000 mg mL-1) were used against pathogens. Ciprofloxacin was used as positive control in case of bacterial strains and Colfrimazol was used...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ayurvedic; Euphorbiaceae; Labeena; Secondary metabolites; Indigenous.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582020000100211
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BIOLOGICAL ACTIVITIES AND NOVEL APPLICATIONS OF CHALCONES Planta Daninha
DÍAZ-TIELAS,C.; GRAÑA,E.; REIGOSA,M.J.; SÁNCHEZ-MOREIRAS,A.M..
ABSTRACT This review provides information on the biological activities of chalcones (whether natural or synthetic derivatives) on different organisms, as well as an overview of the functions and possible new applications of these plant secondary metabolites on crop protection, as eco-friendly pesticides and weed control agents. Naturally occurring chalcones have been used in traditional medicine for many years; however, recent scientific advances have shown that these molecules have a broad range of biological activities in a variety of organisms. A review on the major sources of chalcones and the main molecular events involved in the modes of action of these natural products is achieved. Chalcones are molecules with a broad spectrum of biological...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Mode of action; Secondary metabolites; Crop protection; Pesticide; Fungicide; Antiviral; Phytotoxicity.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582016000300607
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