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Coutinho,Tania Alen; Bernardi,Mari Lourdes; Cardoso,Marisa Ribeiro de Itapema; Borowski,Sandra Maria; Moreno,Andrea Micke; Barcellos,David Emilio Santos Neves de. |
Three comparative assays were performed seeking to improve the sensitivity of the diagnosis of Bordetella bronchiseptica infection analyzing swine nasal swabs. An initial assay compared the recovery of B. bronchiseptica from swabs simultaneously inoculated with B. bronchiseptica and some interfering bacteria, immersed into three transport formulations (Amies with charcoal, trypticase soy broth and phosphate buffer according to Soerensen supplemented with 5% of bovine fetal serum) and submitted to different temperatures (10ºC and 27ºC) and periods of incubation (24, 72 and 120 hours). A subsequent assay compared three selective media (MacConkey agar, modified selective medium G20G and a ceftiofur medium) for their recovery capabilities from clinical... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bordetella bronchiseptica; Swine; Transport media; Selective media; PCR. |
Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300009 |
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