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Use of selective media for detection of cephalothin-resistant bacteria in surgical patients BJID
Melo,Sérgio A.C.; Castro,Eduardo A.R.; Ponce-de-Leon,Antônio C.; Pereira,José A.A..
Bacterial colonization in hospitalized patients is an important step in nosocomial infections. Frequent employment of antimicrobials can modify patients' normal microbiota, favoring colonization and infection by antimicrobial-resistant microorganisms. First-generation cephalosporins are frequently used as prophylactic antibiotics in surgery. Intestinal, oropharyngeal and skin colonization by cephalothin-resistant microorganisms were studied in 60 pre-operative patients at the Hospital Universitário Pedro Ernesto. Feces were cultured in Eosin-methylene blue medium containing 32 µg/mL of cephalothin. Swabs obtained from the oropharynx and from skin were inoculated in cistein-lactose electrolytes-deficient medium containing 32 µg/mL of cephalothin. Isolated...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Selective media; Cephalotin resistance; Surgical patients.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702004000300001
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Performance of transport and selective media for swine Bordetella bronchiseptica recovery and it comparison to polymerase chain reaction detection BJM
Coutinho,Tania Alen; Bernardi,Mari Lourdes; Cardoso,Marisa Ribeiro de Itapema; Borowski,Sandra Maria; Moreno,Andrea Micke; Barcellos,David Emilio Santos Neves de.
Three comparative assays were performed seeking to improve the sensitivity of the diagnosis of Bordetella bronchiseptica infection analyzing swine nasal swabs. An initial assay compared the recovery of B. bronchiseptica from swabs simultaneously inoculated with B. bronchiseptica and some interfering bacteria, immersed into three transport formulations (Amies with charcoal, trypticase soy broth and phosphate buffer according to Soerensen supplemented with 5% of bovine fetal serum) and submitted to different temperatures (10ºC and 27ºC) and periods of incubation (24, 72 and 120 hours). A subsequent assay compared three selective media (MacConkey agar, modified selective medium G20G and a ceftiofur medium) for their recovery capabilities from clinical...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bordetella bronchiseptica; Swine; Transport media; Selective media; PCR.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300009
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