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	Provedor de dados BJMBR (1) Electron. J. Biotechnol. (1) Autor Bendit,I. (1) Cazzola,María Laura (1) Giorgi,R.R. (1) Petruccelli,Silvana (1) Sanabani,S.S. (1) Souza,A.C.M.F. (1) Souza,D.R.V. (1) Mais... Palavra-chave Semi-quantitative PCR (2) Amplification (1) Bt maize (1) Genetically modified organisms (1) MYCN (1) Neuroblastoma (1) RR-soybean (1) Real-time PCR (1) Transgenic food (1) Mais... Tipo do documento Info:eu-repo/semantics/other (1) Journal article (1) Ano 2009 (1) 2006 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última The performance of semi-quantitative differential PCR is similar to that of real-time PCR for the detection of the MYCN gene in neuroblastomas BJMBR Souza,A.C.M.F.; Souza,D.R.V.; Sanabani,S.S.; Giorgi,R.R.; Bendit,I.. Amplification of the MYCN gene in neuroblastomas is a potent biological marker of highly aggressive tumors, which are invariably fatal unless sound clinical management is applied. To determine the usefulness of semi-quantitative differential PCR (SQ-PCR) for accurate quantification of MYCN gene copy number, we evaluated the analytical performance of this method by comparing the results obtained with it for 101 tumor samples of neuroblastoma to that obtained by absolute and relative real-time PCR. Similar results were obtained for 100 (99%) samples, no significant difference was detected between the median log10 MYCN copy number (1.53 by SQ-PCR versus 1.55 by absolute real-time PCR), and the results of the two assays correlated closely (r = 0.8, Pearson... Tipo: Info:eu-repo/semantics/other Palavras-chave: Neuroblastoma; Semi-quantitative PCR; Real-time PCR; MYCN; Amplification. Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000900004 Semiquantitative analysis of genetically modified maize and soybean in food Electron. J. Biotechnol. Cazzola,María Laura; Petruccelli,Silvana. The aim of this study was to analyze quantitatively the presence of genetically modified organism in food with different composition and degree of processing. Total DNA was extracted by Dellaporta's method and GMO analysis was performed using two consecutives PCR reactions with specie specific primers (IVR and LE), screening primers (35S) and transgen specific primers (CRY and EPSPS). The quantification within the sensitivity establish by the EU was possible only in some foods (ice-cream, flours, soybean isolates and concentrates, starch). Samples with high lipid content or subjected to intense thermal treatments (such as some snacks, mayonnaise, creamy soup) could not be amplified mainly due to the presence of PCR inhibitors. Therefore the method was... Tipo: Journal article Palavras-chave: Bt maize; Genetically modified organisms; Semi-quantitative PCR; RR-soybean; Transgenic food. Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000300028 Registros recuperados: 2 Primeira ... 1 ... Última

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