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	Provedor de dados BJMBR (1) Genet. Mol. Biol. (1) Autor Barabás,É. (1) Horváth,A. (1) Khamnamtongand,Bavornlak (1) Klinbunga,Sirawut (1) Menasveta,Piamsak (1) Nagy,K. (1) Preechaphol,Rachanimuk (1) Várkonyi,V. (1) Mais... Palavra-chave Semiquantitative RT-PCR (2) EST (1) HIV (1) IFN-gamma mRNA (1) Ovarian development (1) Penaeus monodon (1) Quantitative competitive RT-PCR (1) SSH (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 2010 (1) 2001 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Comparison of the quantitative competitive and semiquantitative RT-PCR methods for the determination of interferon-gamma mRNA levels in AIDS-free HIV-infected individuals BJMBR Barabás,É.; Nagy,K.; Várkonyi,V.; Horváth,A.. IFN-gamma mRNA expression was evaluated in nonstimulated peripheral blood mononuclear cells (PBMC) of HIV-infected and seronegative individuals using quantitative competitive and semiquantitative RT-PCR and the sensitivity of these methods was compared. A significant correlation was found between quantitative competitive and semiquantitative RT-PCR in samples of both HIV-seronegative (P = 0.004) and HIV-infected individuals (P = 0.0004). PBMC from HIV-infected individuals presented a remarkable increase of IFN-gamma mRNA expression, as determined by both types of RT-PCR methods. Semiquantitative RT-PCR even without an internal standard is also acceptable for measuring cytokine mRNA expression, but less reliable if small amounts are quantified. Moreover, we... Tipo: Info:eu-repo/semantics/other Palavras-chave: Quantitative competitive RT-PCR; Semiquantitative RT-PCR; IFN-gamma mRNA; HIV. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001000006 Isolation and characterization of genes functionally involved in ovarian development of the giant tiger shrimp Penaeus monodon by suppression subtractive hybridization (SSH) Genet. Mol. Biol. Preechaphol,Rachanimuk; Klinbunga,Sirawut; Khamnamtongand,Bavornlak; Menasveta,Piamsak. Suppression subtractive hybridization (SSH) libraries between cDNA in stages I (previtellogenic) and III (cortical rod) ovaries of the giant tiger shrimp (Penaeus monodon) were established. In all, 452 ESTs were unidirectionally sequenced. Sequence assembly generated 28 contigs and 201 singletons, 109 of which (48.0%) corresponding to known sequences previously deposited in GenBank. Several reproduction-related transcripts were identified. The full-length cDNA of anaphase promoting complex subunit 11 (PmAPC11; 600 bp with an ORF of 255 bp corresponding to a polypeptide of 84 amino acids) and selenoprotein M precursor (PmSePM; 904 bp with an ORF of 396 bp corresponding to a polypeptide of 131 amino acids) were characterized and reported for the first time... Tipo: Info:eu-repo/semantics/article Palavras-chave: EST; SSH; Penaeus monodon; Ovarian development; Semiquantitative RT-PCR. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400014 Registros recuperados: 2 Primeira ... 1 ... Última

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