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The combined analysis as the best strategy for Dual RNA-Seq mapping 74
Espindula,Eliandro; Sperb,Edilena Reis; Bach,Evelise; Passaglia,Luciane Maria Pereira.
Abstract In Dual RNA-Seq experiments the simultaneous extraction of RNA and analysis of gene expression data from both interacting organisms could be a challenge. One alternative is separating the reads during in silico data analysis. There are two main mapping methods used: sequential and combined. Here we present a combined approach in which the libraries were aligned to a concatenated genome to sort the reads before mapping them to the respective annotated genomes. A comparison of this method with the sequential analysis was performed. Two RNA-Seq libraries available in public databases consisting of a eukaryotic (Zea mays) and a prokaryotic (Herbaspirillum seropediceae) organisms were mixed to simulate a Dual RNA-Seq experiment. Libraries from real...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Dual RNA-Seq; Sequential analysis; Combined analysis; Mapping strategies.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000500803
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Lead and zinc in the structure of organic and mineral soil components 90
Kummer,Larissa; Melo,Vander de Freitas; Barros,Yara Jurema.
In addition to the more reactive forms, metals can occur in the structure of minerals, and the sum of all these forms defines their total contents in different soil fractions. The isomorphic substitution of heavy metals for example alters the dimensions of the unit cell and mineral size. This study proposed a method of chemical fractionation of heavy metals, using more powerful extraction methods, to remove the organic and different mineral phases completely. Soil samples were taken from eight soil profiles (0-10, 10-20 and 20-40 cm) in a Pb mining and metallurgy area in Adrianópolis, Paraná, Brazil. The Pb and Zn concentrations were determined in the following fractions (complete phase removal in each sequential extraction): exchangeable; carbonates;...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Isomorphic substitution; Sequential analysis; Iron oxide; Kaolinite.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832013000200015
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