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Registros recuperados: 88
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Breeding potential of S4 maize lines in topcrosses for agronomic and forage traits 1
Marcondes, Mariana Martins; Faria, Marcos Ventura; Mendes, Marcelo Cruz; Gabriel, Andre; Neiverth, Victor; Zocche, Jean Carlos.
This study aimed to evaluate the performance of 46 maize lines (S4) obtained from crosses between the commercial hybrids Penta x P30F53 in topcrosses with the commercial simple cross hybrid Dow8460 (tester) and checks (hybrids Penta, P30F53, Dow8460 and Status). The grain yield was evaluated in two environments in Guarapuava, Paraná State, and the effects of genotype, environment and genotype x environment interaction were significant. The grain yield of the topcross hybrids ranged from 8,416 to 13,428 kg ha-1. The agronomic characteristics of the forage and the bromatological characteristics of the silage were evaluated in environment 1. The green mass yield of the forage ranged from 48,767 to 87,714 kg ha-1 and the dry mass yield ranged from 14,749 to...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agronomia; Fitotecnia; Melhoramento Genético Vegetal Zea mays; Acid detergent fiber; Neutral detergent fiber; Silage; Plant breeding Melhoramento Genético de Milho.
Ano: 2016 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/28307
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Efeito de dietas semi-simplificadas formuladas com subprodutos de mandioca ensilados ou não sobre o desempenho e características de carcaça de coelhos - doi: 10.4025/actascianimsci.v33i1.9643 2
Oliveira, Andréia Fróes Galuci; Universidade Estadual de Maringá (UEM); Scapinello, Cláudio; Universidade Estadual de Maringá (UEM); Martins, Elias Nunes; Universidade Estadual de Maringá (UEM); Jobim, Clóves Cabreira; Universidade Estadual de Maringá (UEM); Monteiro, Ana Carolina; Universidade Estadual de Maringá (UEM); Figueira, Josianny Limeira; Universidade Estadual de Maringá (UEM).
Foram avaliados o desempenho da desmama ao abate e as características de carcaça de 144 coelhos da raça Nova Zelândia Branco, oriundos de matrizes alimentadas com dietas referência ou semi-simplificada com 79,83% de subprodutos de mandioca, distribuídos em gaiolas, em esquema fatorial 2 x 3 (duas rações fornecidas às matrizes e láparos até a desmama versus três rações fornecidas aos animais da desmama até o abate) com 12 repetições e dois animais por unidade experimental. As dietas fornecidas, após a desmama, consistiram de uma referência e duas semi-simplificadas com 81,63% de subprodutos de mandioca ensilados ou não antes da sua incorporação. Coelhos cujas mães foram alimentadas com dieta semi-simplificadas se apresentaram mais leves à desmama que os...
Tipo: Pesquisa de campo; análise laboratorial Palavras-chave: Zootecnia - Nutrição de Não-Ruminantes farinha de varredura de mandioca; Feno do terço superior da rama de mandioca; Silagem 5.04.00.00-2 cassava meal; Cassava upper third foliage hay; Silage.
Ano: 2011 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/9643
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Mesquite pod meal in sheep diet: intake, apparent digestibility of nutrients and nitrogen balance 2
Santos, Edileusa de Jesus do; Pereira, Mara Lúcia Albuquerque; Almeida, Paulo José Presidio; Moreira, Jeruzia Vitória; Souza, Andréia Carolina Santos de; Pereira, César Augusto Ramos.
Eight Santa Ines sheep were assigned to two 4 x 4 Latin squares, to evaluate the effects of replacing elephant grass silage with different levels of mesquite pod meal (MDM) (15, 30 and 45% DM) on intake, apparent digestibility of dry matter (DM), organic matter (OM), crude protein (CP), ether extract (EE), acid detergent fiber (ADF), neutral detergent fiber (NDF), total carbohydrates (TC) and non-fiber carbohydrates (NFC) and the nitrogen balance. There was a linear increase (p < 0.05) in the intake of DM, OM, CP, ADF, NDF, NFC and TC according to the addition of MPM to the diet. The digestibility of DM, OM and CP increased (p < 0.05) with the addition of MDM. We observed a positive linear effect (p < 0.05) for the nitrogen intake. The addition of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.00.00.00-4; 5.04.00.00-2 forage conservation; Prosopis juliflora; Silage.
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/24466
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Aerobic stability in corn silage (Zea maysL.) ensiled with different microbial additives 2
Bumbieris Junior, Valter Harry; Guimarães, Vinícius André de Pietro; Fortaleza, Ana Paula de Souza; Massaro Junior, Fernando Luiz; Moraes, Gabriella Jorgette de; Meza, Diego Armando Rojas.
  This study aimed to assess different microbial additives, regarding the efficiency of aerobic stability in corn silages. The corn hybrid used for silages production was the DKB 310. The treatments consisted of: 1) control treatment without any microbial additive; 2) Treatment with LPPA composed of: Lactobacillus plantarum CCT 0580 3.1 x 1010 CFU g-1 and Propionibacterium acidipropionici CCT 4843 3.1 x 1010 CFU g-1; 3) Treatment with Inoculum, composed of Bacillus subtilis CCT 0089 3.0 x 109 CFU g-1, Lactobacillus plantarum CCT 0580 1.2 x 1010 CFU g-1 and Propionibacterium acidipropionici CCT 4843 1.5 x 1010 CFU g-1 and 4) Treatment LB, composed only of Lactobacillus buchneri CCT 3746 2.6 x 1010 CFU g-1. The experimental design was completely randomized...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.04.04.01-6 A aerobiosis; Silage; Fermentation; Nutritional quality..
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/34426
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Phenolic acids and ruminal parameters of different varieties of sugarcane in natura or ensiled 2
Spínola, Jaime Emerson Laranjeira; Tavares dos Reis, Sidnei; Junqueira de Sales, Eleuza Clarete; Monção, Flávio Pinto; Rigueira, João Paulo Sampaio; Delvaux Júnior, Nelson de Abreu.
This study aimed to determine and measure the phenolic acids in four varieties of sugarcane (RB 855536, RB 765418, SP 80-1842 and SP 80-1816) as fresh and ensiled, and assess the influence of these acids on in situ degradability of dry matter and neutral detergent fiber. The experimental design was completely randomized in a factorial design. For the fraction of dry matter (DM), varieties RB 855536 and SP 80-1842 had average 17.80% higher compared to others. The SP80-1842 and RB 855536 varieties did not differ (p > 0.05), with an average of DM effective degradability of 33.70%. For silage, the highest levels of p-coumaric acid were found in the variety RB 855536, which was 24.64% higher than the varieties RB 765418 and SP 80-1842 (average 12.75%) and...
Tipo: Info:eu-repo/semantics/article Palavras-chave: 5.04.03.00-1 digestibility; Fermentation; Lignin; Silage; Nutritional value.
Ano: 2017 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/31470
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Additives in ensiling palisade grass managed under grazing intensities 2
Macedo, Felipe Barros; Luz, Pedro Henrique de Cerqueira; Faria, Letícia de Abreu; Braga, Gustavo José; Peternelli, Maurício; Herling, Valdo Rodrigues.
Conservation of summer forage excess represents a management strategy to meet animals' needs for dry matter in the shortage period, but has been poorly studied. Silage can be used for this purpose. This study analyzed the production of palisade grass silage from pasture subjected to different grazing intensities with and without additive, determining losses by gases and effluents and chemical composition of silage. The experiment was a 4 x 3 factorial completely randomized design, with four replications. The factors were: 1st – herbage allowance of 5% (5 kg dry matter 100 kg-1 of animal weight day-1), 10, 15 and 20%. The pasture was managed under rotational stocking with 35-day grazing cycles (7 days of occupation and 28 days of rest) and 2nd - additives:...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Inoculant; Herbage allowance; Citrus pulp; Silage; Experimental silo..
Ano: 2015 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/27590
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Protein and carbohydrate fractionation of Piata palisadegrass silage of brans from biodiesel industry 2
Epifanio, Patrícia Soares; Instituto Federal Goiano; Costa, Kátia Aparecida de Pinho; Instituto Federal Goiano; Teixeira, Daniel Augusto Alves; Instituto Federal Goiano; Fernandes, Patrick Bezerra; Instituto Federal Goiano; Okada, Eliane Sayuri Miyagi; Universidade Federal de Goiás; Peron, Hugo Jayme Mathias Coelho; Universidade Federal de Goiás.
This study aimed to determine the protein and carbohydrate fractionation of Piata palisadegrass silage of brans from biodiesel industry. The experiment was developed in the Federal Institute of Goiás State, Campus Rio Verde, with a completely randomized experimental design, four replications, in a 4 x 4 factorial scheme, with four oleaginous brans (cotton, sunflower, soybean and canola) and four levels of inclusion (0, 5, 10 and 15%). The results showed that brans from biodiesel industry are good sources of additives for ensiling thePiata palisadegrass, by considerably improving fractions of proteins and carbohydrates. However, the soybean meal proved to be more efficient by having higher fractions of proteins and carbohydrates A+B1 and lower fractions C,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Additive; Brachiaria brizantha; CNCPS; Silage.
Ano: 2014 URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/21646
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Annual production of grass silage for biogas: effects of fibrolytic enzyme additives on ensilage efficiency and specific methane yields 111
Nolan, Pearl; Doyle, Evelyn; O'Kiley, Padraig.
The aims of this study were to quantify the effects of fibrolytic enzyme additives applied to each of four consecutive cuts of unwilted grass at ensiling on ensilage characteristics and specific CH4 yields (SMY) per unit mass and per unit land area. In light of the importance of the primary growth yield, the effects of the timing of Cut 1 were also investigated. Furthermore, the mass-SMY and area-SMY effect of any effluent produced during ensilage was determined.At each of four cuts that comprised annual growth, samples from four replicate plots of Lolium perenne and of Phleum pratense were untreated (control) or were treated with either of two fibrolytic enzymes (ENZ 1 and ENZ 2) prior to ensiling for 120 days. The mass-SMY of silages and effluents were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Grass yield; Silage; Fibre; Enzymes; Effluent; CH4 yield per unit land area.
Ano: 2018 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/4159
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Performance of supplementary irrigation systems for corn silage in the sub-humid areas 111
Mostafa, Harby M. S.; Derbala, Asaad.
In the humid and sub-humid areas, agricultural production is largely rain fed and this needs to be urgently supplemented by irrigation practice if the country is to meet its food demand.  A two years study was carried out at the experimental site of the Institute of Agricultural Technology and Biosystems Engineering, Johan Heinrich von Thünen Institute (vTI), Braunschweig, Germany to compare performance of maize crop for silage production using three different irrigation systems; rain fed, drip and rain-gun sprinkler.  Growth parameters such as plant height, stem diameter were measured.  The total yields of silage were obtained for all treatments at the harvesting.  The experimental results reveal that total yields obtained from different treatments were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Silage; Performance; Drip; Rain-gun; Rain-fed irrigation; Water use efficiency.
Ano: 2013 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/2572
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Performance of supplementary irrigation systems for corn silage in the sub-humid areas 111
Harby M. S. Mostafa; Lecturer of Agricultural Eng.; Asaad Derbala; Assoc. Prof. of Agric. Engi..
In the humid and sub-humid areas, agricultural production is largely rain fed and this needs to be urgently supplemented by irrigation practice if the country is to meet its food demand.  A two years study was carried out at the experimental site of the Institute of Agricultural Technology and Biosystems Engineering, Johan Heinrich von Thünen Institute (vTI), Braunschweig, Germany to compare performance of maize crop for silage production using three different irrigation systems; rain fed, drip and rain-gun sprinkler.  Growth parameters such as plant height, stem diameter were measured.  The total yields of silage were obtained for all treatments at the harvesting.  The experimental results reveal that total yields obtained from different treatments were...
Palavras-chave: Silage; Performance; Drip; Rain-gun; Rain-fed irrigation; Water use efficiency.
Ano: 2013 URL: http://www.cigrjournal.org/index.php/Ejounral/article/view/2572
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Chemical composition and fermentation characteristics of sugar cane silage enriched with detoxified castor bean meal 45
Oliveira,A.C.; Garcia,R.; Pires,A.J.V.; Oliveira,H.C.; Almeida,V.V.S.; Silva,R.R.; Nascimento Filho,C. Souza; Abreu Filho,G..
This experiment was designed to evaluate the effects of the inclusion of detoxified castor bean meal (CM) content on the chemical-bromatological composition, as well as the fermentation characteristics and dry matter losses of sugarcane silage. The treatments consisted of four levels (0, 7, 14 and 21% natural matter) of addition of castor bean meal. The design was completely randomized, with five replicates. The material was ensiled in PVC silos of 50-cm in height and 10-cm in diameter, which were opened after 60 days. The density of fodder maintained in the silos was equal to 750kg of natural matter/m3. In order to quantify the gas and effluent losses, the silos were weighed at the beginning and at the end of the experiment. The addition of castor bean...
Tipo: Info:eu-repo/semantics/article Palavras-chave: By-product; Ethanol; Gases; Saccharum officinarum L; Silage.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352015000100181
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Occurrence of Listeria monocytogenes in silages assessed by fluorescent in situ hybridization 45
Oliveira,M.; Guerra,M.; Bernardo,F..
Avaliou-se a qualidade microbiológica da silagem produzida em Portugal e otimizaram-se alguns aspectos relacionados com a eficácia de detecção de Listeria monocytogenes mediante protocolo baseado na técnica de hibridação in situ fluorescente (FISH) para detecção directa desse microrganismo em amostras de silagens. O protocolo foi aplicado a 74 amostras, em simultâneo com o método bacteriológico convencional. Este último permitiu a detecção de L. monocytogenes em 11 silos (15%). Por meio do protocolo FISH, observou-se que 22 silos (29,7%) se encontravam contaminados. O método FISH apresentou precisão (77,0%), especificidade (92,5%) e sensibilidade (72,7%) elevadas, sendo adequado para a análise microbiológica presuntiva de silagens, uma vez que é um método...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Silage; Listeriosis; Fluorescent in situ hybridization; Listeria monocytogenes; Portugal.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352008000100038
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Dietary utilisation of protein and energy from fresh and ensiled coffee pulp by the Nile tilapia, Oreochromis niloticus 52
Moreau,Yann; Arredondo,Jose-Luis; Perraud-Gaime,Isabelle; Roussos,Sevastianos.
Dietary protein and energy utilisation of diets containing fresh and ensiled coffee pulp were studied on 3.2 ± 0.2 g Nile tilapia for 28 days. Diets formulation and feeding were designed on the basis of daily dietary protein and energy allowance. A control diet A (100 % protein and 100 % energy allowance) corresponding to 15 g CP kg-1 day-1 and 750 kJ kg-1 day-1, a low protein control diet B (80 % protein and 100 % energy allowance), two diets C and E (100 % protein and 100 % energy allowance) where 20 % of protein were supplied by coffee pulp, and two diets D and F with the same amount of coffee pulp than in C and E and supplementation in non-protein energy. Inclusion of coffee pulp in the diet strongly impaired growth and feed utilisation. Silage process...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffee pulp; Silage; Aquaculture; Cichlids; Feedstuff; Fish.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000200014
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Prediction of the Nutritive Value of Pasture Silage by Near Infrared Spectroscopy (NIRS) 117
Restaino,Ernesto A; Fernández,Enrique G; La Manna,Alejandro; Cozzolino,Daniel.
The aim of this study was to investigate the use of near infrared reflectance (NIRS) spectroscopy to predict the nutritive value of silages from pastures and to assess the effect of silage structure type (e.g. bunker and bag silos) on the NIRS predictions. Samples (n = 120) were sourced from commercial farms and analyzed in a NIRS monochromator instrument (NIR Systems, Silver Spring, Maryland, USA) using wavelengths between 400 and 2500 nm in reflectance. Calibration models were developed between chemical and NIRS spectral data using partial least squares (PLS) regression. The coefficients of determination in calibration (R²) and the standard error in cross validation (SECV) were 0.73 (SECV: 1.2%), 0.81 (SECV: 2.0%), 0.75 (SECV: 6.6%), 0.80 (SECV: 6.7%),...
Tipo: Journal article Palavras-chave: Silage; Nutritive value; Near infrared reflectance spectroscopy; Pastures.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000400011
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Prediction of the Chemical Composition and Fermentation Parameters of Pasture Silage by Near Infrered Reflectance Spectroscopy (NIRS) 117
Ibáñez,Lorena; Alomar,Daniel.
The capability of near infrared reflectance spectroscopy (NIRS) was evaluated to predict the content of total ash (TA), crude protein (CP), crude fiber (CF), neutral detergent fiber (NDF), acid detergent fiber (ADF) and metabolizable energy (ME); as well as pH and ammonia nitrogen content (N-NH3), in pasture silage, with and without additives. Nine hundred and twenty dried and ground samples of pasture silage, with known chemical composition, were scanned over the visible and NIR region (400 to 2500 nm) at 2 nm intervals. Calibration equations were developed by modified partial least square regression models (MPLS) with different mathematical treatments and light scatter correction as standard normal variation and Detrend (SNV &amp; D) of the spectra....
Tipo: Journal article Palavras-chave: Nutritional composition; Silage; Pasture; Spectroscopy; NIRS.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000400005
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Evaluation of sugar cane bagasse subjected to haying and ensiling 64
Pereira,Rosana Cristina; Evangelista,Antônio Ricardo; Muniz,Joel Augusto.
This work aimed to evaluate sugar cane bagasse from cachaça production, subjected to hay-making and ensiling. The experiment was conduced at Universidade Federal de Lavras, MG, using the completely random delineation (CRD), with seven treatment and three repetitions. The treatments were constituted of in natura sugar cane bagasse (INB), manually hayed and baled sugar cane bagasse, mechanically hayed and baled, manually baled INB and mechanically baled INB, whole ensiled INB and chopped ensiled INB. Bromatological composition, tampon capacity (TC), pH values and total sugar rate of the INB were evaluated. For the ensilage, there were used cistern silos and for the baling, manual and mechanical balers. The bagasse was baled in natura, or after reaching 89%...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hay; Silage; Sub-product.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542009000600027
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Dynamics of change in fermentation and fatty acid profiles in high moisture alfalfa silage during ensiling at different temperatures 65
Liu,Qinhua; Dong,Zhihao; Shao,Tao.
ABSTRACT: The objective of present study was to investigate the dynamics of change in fermentation and fatty acid profiles in high moisture (DM=232g/kg FW) alfalfa silages during ensiling at 45°C, 30°C and 15°C. After ensiling for 1, 3, 7, 21, 39 and 65 days, silages was sampled and examined. Lactic fermentation changing into butyric fermentation in silage ensiled at 30°C and 45°C occurred on ensiling for 21 and 65 days, respectively, as accompanied with high ammonia-N content ( >150g/kg N), which subsequently resulted in a sudden increase of pH (P < 0.05). In contrast, the increase of lactic acid content was observed in silage ensiled at 15°C during ensiling for 65 days (P<0.05). As the ensiling temperature rose, considerable losses in total FA,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Silage; Fermentation quality; Fatty acid; Temperature.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000300652
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Adding distiller's grains and molasses on fermentation quality of rice straw silages 65
Yuan,XianJun; Dong,ZhiHao; Desta,Seare Tajebe; Wen,AiYou; Zhu,Xiaoxuan; Rong,Ting; Shao,Tao.
ABSTRACT: Ensilage is a simple and low-cost strategy to enable long term preservation and environmentally friendly utilization of agricultural by-products, such as straws and distiller's grains (DG) for ruminants. Effect of mixing different proportions of DG and rice straw (i.e. 0, 10, 20 or 30% of DG) with or without 5% molasses addition on fermentation and chemical variables of silages was evaluated. The study was conducted as a randomized blocks design in a 4 × 2 factorial arrangement, with three replications, using laboratory silos of 1L capacity (n=24). Despite a significant interaction (P<0.01) between DG and molasses addition was observed for most variables, in general the increased addition of DG linearly decreased the pH value, acetic acid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Distiller's grains; Fermentation quality; Molasses; Rice straw; Silage.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782016001202235
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Ensilaje de pencas de nopal tunero con excretas pecuarias. 32
Alejos de la Fuente, José Isidro.
Con el objetivo de evaluar las características químicas de ensilados de pencas de nopal tunero y excretas pecuarias (pollinaza y estiércol bovino), se realizaron 2 experimentos con arreglos factoriales de tratamientos 4 x 4 en diseños experimentales completamente al azar; las unidades experimentales fueron 64 microensilados (16 tratamientos con 4 repeticiones), para cada experimento; con 6 kg de una mezcla compuesta con pencas de nopal, rastrojo de maíz molido, excretas pecuarias y melaza; ensilados en 4 tiempos: 0, 30 ,60 y 90 días (Factor A), con 4 concentraciones de excretas pecuarias: 0, 20, 30 y 50% (Factor B). Al incrementar el tiempo de ensilaje, aumentaron (P < 0.0001) la PC, carbohidratos no fibrosos (CNF), cenizas (C), EM (Mcal/kg), ácido...
Palavras-chave: Ensilaje; Nopal; Excretas pecuarias; Características químicas; Silage; Prickly pear cladodes; Poultry litter; Cattle manure; Chemical characteristics; Ganadería; Doctorado.
Ano: 2013 URL: http://hdl.handle.net/10521/2054
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Eficiencia de polinización, productividad y calidad de semilla y forraje en híbridos trilineales de maíz 32
Rivas Jacobo, Marco Antonio.
El objetivo fue evaluar y seleccionar líneas elite de maíz; formar y evaluar híbridos trilineales, por su calidad forrajera, considerando calidad de semilla. La investigación se realizó en, Montecillo, Texcoco, Méx., Región Subtropical y Valles Altos. Las variables principales fueron: Aspecto físico de la hoja (AFH), Cualidad Forrajera (CF), Hojas por planta (HPP), Altura de planta (AP), Diámetro de tallo (DT), Mazorcas por planta (MAZP), Rendimiento de grano (RENGR), Hileras por mazorca (HPM), Granos por hilera (GPH), Largo de mazorca (LMZ), Diámetro de mazorca (DMZ), Porcentaje de Granos No Formados (PGNF), Peso de Cien Semillas (PCS), Peso Volumétrico (PV), Porcentaje de Germinación (PG), Rendimiento de, semilla (RS), materia seca de la planta completa...
Palavras-chave: Zea mays L.; Polinización; Forraje; Materia seca; Ensilado; Semilla; Pollination; Forage; Dry matter; Silage; Seed; Doctorado; Producción de semillas.
Ano: 2010 URL: http://hdl.handle.net/10521/71
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