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	Provedor de dados Genet. Mol. Biol. (2) Autor Bartholomei-Santos,Marlise L. (1) Buchmann,Darine (1) Gill-Langarica,Homar R. (1) Mayek-Pérez,Netzahualcoyotl (1) Muruaga-Martínez,José S. (1) Roratto,Paula A. (1) Rosales-Serna,Rigoberto (1) Santos,Sandro (1) Vargas-Vázquez,M.L. Patricia (1) Mais... Palavra-chave Simple sequence repeats (SSR) (2) Amplified fragment length polymorphisms (AFLP) (1) Chimeric PCR product (1) Genetic relationships (1) Germplasm bank (1) Microsatellite isolation (1) Phaseolus vulgaris L. (1) Recombination (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 2011 (1) 2008 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última PCR-mediated recombination in development of microsatellite markers: mechanism and implications Genet. Mol. Biol. Roratto,Paula A.; Buchmann,Darine; Santos,Sandro; Bartholomei-Santos,Marlise L.. Protocols for microsatellite-enrichment libraries have been widely applied to several species in order to supply the most informative molecular markers for population and inbreeding studies. One drawback of these protocols is the ratio of designed primer pairs that fail to amplify the expected fragment, even after exhaustive optimization attempts. A possible cause of unsuccessful microsatellite primers may be that such loci are artifacts resulting from chimeric PCR products, instead of real genomic sequences. The microsatellite-enriched library constructed for Aegla longirostri (Crustacea, Decapoda, Anomura) showed that 29% of sequenced clones were chimeric products because these sequences shared one of the flanking regions around the same repeat motif but... Tipo: Info:eu-repo/semantics/other Palavras-chave: Chimeric PCR product; Microsatellite isolation; Recombination; Simple sequence repeats (SSR). Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100012 Genetic diversity analysis of common beans based on molecular markers Genet. Mol. Biol. Gill-Langarica,Homar R.; Muruaga-Martínez,José S.; Vargas-Vázquez,M.L. Patricia; Rosales-Serna,Rigoberto; Mayek-Pérez,Netzahualcoyotl. A core collection of the common bean (Phaseolus vulgaris L.), representing genetic diversity in the entire Mexican holding, is kept at the INIFAP (Instituto Nacional de Investigaciones Forestales, Agricolas y Pecuarias, Mexico) Germplasm Bank. After evaluation, the genetic structure of this collection (200 accessions) was compared with that of landraces from the states of Oaxaca, Chiapas and Veracruz (10 genotypes from each), as well as a further 10 cultivars, by means of four amplified fragment length polymorphisms (AFLP) +3/+3 primer combinations and seven simple sequence repeats (SSR) loci, in order to define genetic diversity, variability and mutual relationships. Data underwent cluster (UPGMA) and molecular variance (AMOVA) analyses. AFLP analysis... Tipo: Info:eu-repo/semantics/article Palavras-chave: Phaseolus vulgaris L.; Amplified fragment length polymorphisms (AFLP); Germplasm bank; Genetic relationships; Simple sequence repeats (SSR). Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400012 Registros recuperados: 2 Primeira ... 1 ... Última

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