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GENOMIC INTEGRITY EVALUATION IN SPERM OF CHOROMYTILUS CHORUS (MOLINA, 1782) BY COMET ASSAY Gayana
Lafarga-De la Cruz,Fabiola; Valenzuela-Bustamante,Myriam; Dupré,Enrique; del Río-Portilla,Miguel A; Gallardo-Escárate,Cristián.
The comet assays or single cell gel electrophoresis is a simple, rapid, relatively inexpensive and very sensitive method that has been recently widely used to evaluate genomic integrity (or DNA damage) in aquatic animals exposed to diverse physical and chemical agents. However, the accuracy to evaluate comets assay has been the major obstacle in order to determine specific damage undergone cellular stress. The aim of this study was to evaluate DNA fragmentation in sperm cells based in fluorescence image analysis. The species used was the giant mussel Choromytilus chorus. The in vitro experiments were carried out through oxidative stress with five hydrogen peroxide concentrations (0, 50, 100, 500 and 1000 mM). To evaluate DNA damage two groups of parameters...
Tipo: Journal article Palavras-chave: Single cell gel electrophoresis; DNA damage; Image analysis.
Ano: 2008 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-65382008000100006
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Biological effects of contaminants: Assessing DNA damage in marine species through single-cell alkaline gel electrophoresis (comet) assay. ArchiMer
Bean, Tp; Akcha, Farida.
Single cell gel electrophoresis (SCGE or comet) assay allows quantification of DNA damage in individual cells and is an ideal tool for use within biological monitoring programmes. Comet assay can be used on a range of cell types including somatic, reproductive (gametes) or circulatory cells in many different species including both marine bivalves and flatfish. The assay can be employed with simple equipment available in most laboratories, is sensitive to environmentally relevant levels of DNA damage (Frenzilli and Lyons, 2009), accurately demonstrates a linear dose response to exposure (Collins et al., 1996), and can be adapted for use on most nucleated cell types. This document concentrates on the simplest and most repeatable method of comet assay in...
Tipo: Text Palavras-chave: Comet assay; Single cell gel electrophoresis; DNA damage; Genotoxicity; Biological effects; Bivalves; Flatfish; Integrated monitoring.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00587/69902/67807.pdf
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