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| Registros recuperados: 30 | |
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| Liu,Yu; Duan,Xiaoyan; Chen,Si; He,Hua; Liu,Xiaolin. |
| Abstract Based on RNA-seq analysis, we recently found that the bovine NCAPG (non-SMC condensin I complex, subunit G) gene is differentially expressed during development of the longissimus muscle. In the present study, we validated this result and, using quantitative real-time PCR analysis, identified two adjacent genes, LCORL and DCAF16, that are more abundant in fetal muscle tissue; further analysis of tissue-specific expression patterns indicated high abundance of NCAPG in muscle. Since no polymorphisms were detected in a previous study of Qinchuan cattle, we extended our investigation to examine the occurrence of single-nucleotide polymorphisms (SNPs) in the NCAPG gene. Three SNPs, i.e., one located in the intron region (g47747: T > G), a synonymous... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Association analysis; Growth traits; Longissimus muscle; NCAPG expression; Single nucleotide polymorphism. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000400450 |
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| Fernández,María E.; Goszczynski,Daniel E.; Lirón,Juan P.; Villegas-Castagnasso,Egle E.; Carino,Mónica H.; Ripoli,María V.; Rogberg-Muñoz,Andrés; Posik,Diego M.; Peral-García,Pilar; Giovambattista,Guillermo. |
| During the last decade, microsatellites (short tandem repeats or STRs) have been successfully used for animal genetic identification, traceability and paternity, although in recent year single nucleotide polymorphisms (SNPs) have been increasingly used for this purpose. An efficient SNP identification system requires a marker set with enough power to identify individuals and their parents. Genetic diagnostics generally include the analysis of related animals. In this work, the degree of information provided by SNPs for a consanguineous herd of cattle was compared with that provided by STRs. Thirty-six closely related Angus cattle were genotyped for 18 STRs and 116 SNPs. Cumulative SNPs exclusion power values (Q) for paternity and sample matching... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Microsatellite; Single nucleotide polymorphism; Exclusion probability; Genetic identification; Bovine. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200008 |
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| Wortmann,André Castagna; Simon,Daniel; Mazzoleni,Luiz Edmundo; Sander,Guilherme Becker; Francesconi,Carlos Fernando de Magalhães; Nabinger,Débora Dreher; Grott,Camila Schultz; Rech,Tássia Flores; Mazzoleni,Felipe; Lunge,Vagner Ricardo; Bona,Laura Renata de; Milbradt,Tobias Cancian; Silveira,Themis Reverbel da. |
| Abstract Functional dyspepsia and lactose intolerance (adult-type hypolactasia, ATH) are common conditions that may coexist or even be confounded. Their clinical presentation can be similar, however, lactose intolerance does not form part of the diagnostic investigation of functional dyspepsia. Studies on the association between functional dyspepsia and ATH are scarce. This study aimed to evaluate whether ATH is associated with symptoms of functional dyspepsia. Patients fulfilling the Rome III diagnostic criteria for functional dyspepsia underwent genetic testing for ATH. Dyspeptic symptoms were evaluated and scored according to a validated questionnaire. The diagnostic criteria for ATH was a CC genotype for the -13910C/T polymorphism, located upstream of... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bloating; Dyspepsia; Gastrointestinal diseases; Lactose intolerance; Single nucleotide polymorphism. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100092 |
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| Cypriano,Ana Sheila; Alves,Gilda; Ornellas,Antonio Augusto; Scheinkman,José; Almeida,Renata; Scherrer,Luciano; Lage,Claudia. |
| Abstract Susceptibility to cancer ensues in individuals carrying malfunctioning DNA repair mechanisms. The impact of Single Nucleotide Polymorphisms (SNPs) in key DNA repair mechanisms on risk for prostate cancer was investigated in this case-control study. Samples consisted of 110 patients with confirmed prostate cancer and 200 unaffected men, from Rio de Janeiro, Brazil. XPD/Lys751Gln (rs13181), APEX1/Asp148Glu (rs1130409), and RAD51/G135C (rs1801320) SNPs were analyzed by PCR-RFLP. Allelic and genotypic frequencies were calculated and compared by Chi-Square test. The association between SNPs and clinical/epidemiological data was considered significant by Odds Ratio analysis, with IC95% and a p-value≤0.05. Only the XPD/Lys751Gln SNP significantly... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Prostate cancer; Single nucleotide polymorphism; XPD; DNA repair; Gene-environment interaction. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500751 |
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| Qin,Peng; Lin,Yu; Hu,Yaodong; Liu,Kun; Mao,Shuangshuang; Li,Zhanyi; Wang,Jirui; Liu,Yaxi; Wei,Yuming; Zheng,Youliang. |
| Abstract The D-genome progenitor of wheat (Triticum aestivum), Aegilops tauschii, possesses numerous genes for resistance to abiotic stresses, including drought. Therefore, information on the genetic architecture of A. tauschii can aid the development of drought-resistant wheat varieties. Here, we evaluated 13 traits in 373 A. tauschii accessions grown under normal and polyethylene glycol-simulated drought stress conditions and performed a genome-wide association study using 7,185 single nucleotide polymorphism (SNP) markers. We identified 208 and 28 SNPs associated with all traits using the general linear model and mixed linear model, respectively, while both models detected 25 significant SNPs with genome-wide distribution. Public database searches... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aegilops tauschii; Drought resistance; Genome-wide association study; Single nucleotide polymorphism; Wheat. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000300398 |
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| Ferreira,Jéssica Rosset; Faria,Bruna Franciele; Comar Junior,Moacyr; Delatorre,Carla Andréa; Minella,Euclydes; Pereira,Jorge Fernando. |
| Abstract The barley HvAACT1 gene codes for a citrate transporter associated with tolerance to acidic soil. In this report, we describe a single nucleotide polymorphism (SNP) in the HvAACT1 coding region that was detected as T-1,198 (in genotypes with lower root growth on acidic soil) or G-1,198 (greater root growth) and resulted in a single amino acid change (L/V-172). Molecular dynamic analysis predicted that HvAACT1 proteins with L or V-172 were stable, although the substitution led to structural changes within the protein. To evaluate the effect of the SNP on tolerance to acidic soil, barley accessions were separated into haplotypes based on the presence of a 1 kb insertion in the HvAACT1 promoter and a 21 bp insertion/deletion. These markers and the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aluminium tolerance; Citrate transporter; Haplotype; Hordeum vulgare; Single nucleotide polymorphism. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000300480 |
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| Jemaa,Slim Ben; Thamri,Nejia; Mnara,Sofiane; Rebours,Emmanuelle; Rocha,Dominique; Boussaha,Mekki. |
| Abstract To carry out effective genome-wide association studies, information about linkage disequilibrium (LD) is essential. Here, we used medium-density SNP chips to provide estimates of LD in native Tunisian cattle. The two measures of LD that were used, mean r2 and D’, decreased from 0.26 to 0.05 and from 0.73 to 0.40, respectively, when the distance between markers increased from less than 20 Kb to 200 Kb. The decay in LD over physical distance occurred at a faster rate than that reported for European and other indigenous breeds, and reached background levels at less than 500 Kb distance. This is consistent with the absence of strong selective pressure within the Tunisian population and suggests that, in order to be effective, any potential genome-wide... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tunisian cattle; Conservation; Linkage disequilibrium; Effective population size; Single nucleotide polymorphism. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572019000100052 |
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| Dalongeville, Alicia; Andrello, Marco; Mouillot, David; Lobreaux, Stephane; Fortin, Marie-josee; Lasram, Frida; Belmaker, Jonathan; Rocklin, Delphine; Manel, Stephanie. |
| Genetic variation, as a basis of evolutionary change, allows species to adapt and persist in different climates and environments. Yet, a comprehensive assessment of the drivers of genetic variation at different spatial scales is still missing in marine ecosystems. Here, we investigated the influence of environment, geographic isolation, and larval dispersal on the variation in allele frequencies, using an extensive spatial sampling (47 locations) of the striped red mullet (Mullus surmuletus) in the Mediterranean Sea. Univariate multiple regressions were used to test the influence of environment (salinity and temperature), geographic isolation, and larval dispersal on single nucleotide polymorphism (SNP) allele frequencies. We used Moran's eigenvector maps... |
| Tipo: Text |
Palavras-chave: Connectivity; Ecological genetics; Marine fish; Mediterranean Sea; Mullus surmuletus; Seascape genetics; Single nucleotide polymorphism. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00454/56556/75079.pdf |
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| Ripoli,M.V; Wei,S; Rogberg-Muñoz,A; Guo,B.L; Goszczynski,D.E; Fernandez,M.E; Mellucci,L; Lirón,J.P; Villarreal,E; Wei,Y.M; Giovambattista,G. |
| Genetic traceability refers to methods associated with the identification of animals and their products through DNA characterization of individuals, breeds or species. To trace breeds, it is necessary to define the breed groups to analyze, and the most appropriate molecular marker set. The selection of genetic markers depends on the gene frequency distribution, the genetic distance among breeds and the presence of private alleles. In this study, we assessed six single nucleotide polymorphisms (SNPs) located in the DGAT1, TG, LEP, GH, FABP4 and GnRHR genes, as potential genetic markers to be included into a panel for genetic traceability for the identification of breed origin associated with the bovine beef trade. The results of the genetic characterization... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Breed traceability; Genetic traceability; Single nucleotide polymorphism; Chinese cattle; Argentine cattle. |
| Ano: 2013 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332013000300004 |
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| Rebelato,Arnaldo Basso; Caetano,Alexandre Rodrigues. |
| Abstract: Runs of homozygosity (ROHs) are long stretches of homozygous genomic segments, identifiable by molecular markers, which can provide genomic information for accurate estimates to characterize populations, determine evolutionary history and demographic information, estimate levels of consanguinity, and identify selection signatures in production animals. This review paper aims to perform a survey of the works on the efficiency of ROHs for these purposes. Factors such as genetic drift, natural or artificial selection, founder effect, and effective population size directly influence the size and distribution of ROHs along the genome. Individually, genome estimates of consanguinity based on ROHs can be obtained using the FROH index, which is generally... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Autozygosity; FROH; Genotyping; Selection signatures; Single nucleotide polymorphism; SNPs. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000900975 |
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| Tu,Yi-Chen; Wei,Liang-Yuan; Chang,Yi-Ying; Liu,Hsiu-Chou; Lee,Hsien-Hsiung; Yu,Yu-Hsiang; Chen,Ming-Cheng. |
| ABSTRACT The objective of the present study was to investigate the effect of single nucleotide polymorphism (SNP) of the melanocortin 1 receptor (MC1R) gene on plumage coloration in mule ducks. PCR-high-resolution melting analysis (PCR-HRM) and DNA sequencing were used to identify the SNP variability of the MC1R gene in white common ducks. Three non-synonymous SNP (MC1R gene exon 1, c.52G>A, c.376G>A, and c.409G>A) were identified in white Tsaiya ducks. Mating test (white Tsaiya ducks × white Muscovy drakes) in combination with polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was performed to investigate the effect of non-synonymous SNP of different maternal lines on plumage coloration in mule ducks. Genotyping results... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Muscovy drakes; Polymerase chain reaction-restriction fragment length polymorphism; Single nucleotide polymorphism; Tsaiya ducks. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100305 |
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| Registros recuperados: 30 | |
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