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| Registros recuperados: 40 | |
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| Pachioni,Célia Aparecida Stellutti; Mazzer,Nilton; Barbieri,Claudio Henrique; de Camargo,Marcela Regina; Fregonesi,Cristina Elena Prado Teles; do Carmo,Edna Maria; Nozabielli,Andrea Jeanne Lourenço; Martinelli,Alessandra Rezende. |
| The study aimed to verify the physiological injury behavior by stretching the soleus muscle of rats, using a noninvasive experimental model. Twenty-four rats were used and divided into three groups of eight animals: control group (A), group that performed tetanus followed by electrical stimulation and a sudden dorsiflexion of the left paw performed by a device equipped with a mechanism of muscle soleus rapid stretching (B); and a group that only received the tetanus (C). Three days later, the animals were killed, and the soleus muscle was resected and divided into three segments. Morphological changes indicative of muscle damage appeared in all three segments of group B. In a lesser degree, similar changes were also detected in muscles subjected to only... |
| Tipo: Journal article |
Palavras-chave: Disease models; Animal; Rats; Skeletal muscle; Tetany. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400029 |
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| Davatz,Giovanna Castilho; Andreo,Jesus Carlos; Rodrigues,Antonio de Castro; Rosa Júnior,Geraldo Marco; Moraes,Luiz Henrique Rapucci. |
| The apoptosis phenomenon happens in normal tissues and in many pathological conditions as described by several researchers. Most studied in the 70s, this phenomenon remained almost forgotten in the 90s, when, with the advent of more advanced techniques, returned to laboratories. Thus, electronic microscopy, Comet Assays and histochemistry techniques, among others, were utilized in an effort to elucidate in a thorough way, the mechanisms that lead the cell to "suicide". No one can deny the importance of the knowledge of such phenomenon, so as to control its induction or blocking in the treatment of pathologies such as cancer. In this work, the apoptosis study was aimed at denervated muscles in different periods. Furthermore, two types of skeletal striated... |
| Tipo: Journal article |
Palavras-chave: Skeletal muscle; Denervation; Apoptosis; Rat. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000300009 |
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| Acuña,Luis; Suazo Galdames,Iván; Zavando,Daniela; Elgueta,Samanta; Velásquez,Luis; Vilos,Cristian; Cantín,Mario. |
| The administration of microencapsulated drug in a matrix acid poly(lactic-co-glycolic acid) (PLGA) by intramuscular (IM) in humans has been approved by the FDA for various applications though it is not clear what effect they have on the morphological parameters of muscle tissue. The aim of this study was to analyze the morphological changes in the skeletal muscle tissue with their use. We used 12 adult female Sprague Dawley rats (Rattus novergicus) that were injected into their right gastrocnemius muscle belly with: sterile vehicle solution (G1, n = 4), 0.5 mg PLGA microparticle (G2, n = 4) and 0.75 mg PLGA microparticle (G3, n = 4), both dissolved in a sterile vehicle solution. At 14 days post injection the number and diameter of muscle fibers, the level... |
| Tipo: Journal article |
Palavras-chave: Skeletal muscle; Drug delivery; Polymeric microparticles; PLGA. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022011000200016 |
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| de Freitas,Carlos Eduardo A; Freitas,Selma de Bastos Zambelli; Lopes,Francis da Silva; Pai-Silva,Maeli Dal; Piçarro,Ivan da Cruz. |
| The muscles can perform the same function in a specific segment (muscles of fast and slow contraction), and at the same time be antagonistic in relation to muscular action (flexors or extensors). The present research aimed to study the morphology, frequency and metabolism of fiber types and the contractile characteristics of extensor and flexors muscles of rabbit. We studied muscles anterior tibialis (AT), flexor digitorum supeficialis (FDS), extensor digitorum longus (EDL) and posterior tibialis (PT). The muscles were submitted to the techniques HE, NADH-TR and myofibrillar ATPase. In EDL and PT extensor muscles, the frequencies of red (SO + FOG) and white fibers (FG) were 68.77% and 31.23% versus 58.87% and 41.13%, respectively. In the AT and FDS flexor... |
| Tipo: Journal article |
Palavras-chave: Contractile ability; Metabolic; Morphologic; Skeletal muscle. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000400034 |
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| Baldo,C; Ferreira,MJ; Lopes,DS; Izidoro,LFM; Gomes,AO; Ferro,EAV; Hamaguchi,A; Homsi-Brandeburgo,MI; Rodrigues,VM. |
| The damaging effects of neuwiedase, a non-hemorrhagic snake venom metalloproteinase from P-I class, on gastrocnemius muscle are studied herein. Following neuwiedase injection, ultrastructural alterations were detected early showing disarrangement of skeletal muscle fibers (characterized by discontinuity of Z lines), mitochondrial swelling, and disruption of plasma membrane and basal lamina. Degradation of skeletal muscle and the appearance of an amorphous substance, primarily composed of cellular debris, were noted after 24 hours. The presence of neuwiedase at the injection site (detected by immunocytochemistry) revealed highly specific labeling of myofibril components of damaged myocytes. In addition, proteolysis of muscle proteins assayed through... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Skeletal muscle; Neuwiedase; Snake venom metalloproteinases; Bothrops neuwiedi. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300013 |
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| Mesquita-Ferrari,RA; Moraes,CK de; Micocci,KC; Selistre-de-Araújo,HS. |
| ALT-C, an ECD motif (glutamic acid, cysteine, aspartic acid) disintegrin from Bothrops alternatus snake venom, induces α2β1 integrin-mediated signaling and neutrophil chemotaxis. In vitro, in human umbilical vein endothelial cells (HUVEC), ALT-C induces cell proliferation, thus showing an interesting potential for tissue regeneration studies. This work aimed to evaluate the influence of ALT-C in myoblast viability and differentiation. Myoblasts were obtained from hind limb muscles of 3 to 4-day old Wistar rats. The cells were incubated with ALT-C at different concentrations and incubation periods were followed by total RNA isolation. cDNA synthesis and real time polymerase chain reaction (PCR) were performed with primers of myoD as well as of both (slow... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Viability; Myosin heavy chain; Myoblast; Disintegrin; Skeletal muscle; Bothrops alternatus. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200013 |
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| Paim,Tiago do Prado; Viana,Pauline; Tilburg,Mauricio Fraga van; Moura,Arlindo de Alencar; Souza,Jurandir Rodrigues de; McManus,Concepta; Abdalla,Adibe Luiz; Louvandini,Helder. |
| ABSTRACT: Gossypol easily pairs with lysine side chains and enzymes involved in the cellular growth process. The effect of gossypol (a compound present in cotton co-products) in ruminant metabolism and meat quality is not yet clear. This study was undertaken in order to evaluate the effects of cotton co-products in lamb muscle proteome. Twenty-four Santa Inês ram lambs, 5-months old (20.6 ± 1.9 kg), were randomly assigned to four treatments: control (without cottonseed), whole cottonseed, cottonseed meal and high oil cottonseed meal. At 95 days into the experiment, lambs were slaughtered and samples from Longissimus dorsi were collected. Proteins were extracted and analyzed by 2-D electrophoresis. Spots showing a significant effect from the treatment (the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Gossypol; Dehydrogenase; Skeletal muscle; Sheep; Feedlot. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162019001600463 |
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| SOUZA,JANAINA S. DE; BRUNETTO,ERIKA L.; NUNES,MARIA TEREZA. |
| ABSTRACT Iron is an important trace element for proper cell functioning. It is present in cytochromes, hemoglobin and myoglobin (Mb), where it binds to oxygen. It is also an electron acceptor in the respiratory chain. Mb is an 18 kDa heme-protein, highly expressed in skeletal muscle and heart. The expression of several genes involved in the metabolism of iron is post-transcriptionally regulated by this element. Iron was shown to interfere with the polyadenylation step, modifying their poly (A) tail length and, as a consequence, their stability and translation rate. The aim of this study was to investigate whether iron supplementation or long and short-term restriction affects Mb gene and protein expression, as well as Mb mRNA poly(A) tail length, in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cardiac muscle; Iron; Myoglobin; Skeletal muscle. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000602277 |
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| RAMIREZ,BEATRIZ U; RETAMAL,LEIRA; VERGARA,CECILIA. |
| The well-established trophic role of CNTF upon neurons led to performing clinical trials in patients of neurodegenerative diseases. However, trials were suspended due to side effects such as severe weight loss, hyperalgesia, coughing, muscle cramps and pain. So far it is not known how CNTF triggers the problems related to skeletal muscle cramps and pain. CNTF has also been described as a myotrophic factor for denervated skeletal muscles, but the possibility that it affects innervated muscles has also been considered. Since a myotrophic factor could be a valuable tool for treatment of several muscle diseases, we studied the effects of low doses of CNTF delivered systemically by an osmotic pump, over the electrical and mechanical properties of innervated and... |
| Tipo: Journal article |
Palavras-chave: CNTF; Contraction and relaxation time; Denervation; EMG; Fibrillation; Skeletal muscle. |
| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602003000300003 |
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| TIIDUS,PETER M; DELLER,MIRADA; BOMBARDIER,ERIC; GÜL,MUSTAFA; LIU,X. LINDA. |
| This study examined the effects of estrogen supplementation on markers of neutrophil infiltration and damage in skeletal muscle of rats following ischemia. Male and female gonad-intact rats, with or without 14 days of estrogen supplementation were subjected to two hours of hind-limb ischemia and sacrificed at 24, 48 or 72 hours post-ischemia. Control animals were sacrificed without ischemia. Plantaris and red and white gastrocneimus muscles were removed and assayed for myeloperoxidase (MPO), a marker of neutrophil infiltration, and glucose-6-phosphate dehydrogenase (G6PD) and ß-glucuronidase (<img border=0 width=8 height=15 id="_x0000_i1026" src="http://fbpe/img/bres/v38n2-3/existe.gif" align=absbottom>GLU), as markers of muscle damage. Significant... |
| Tipo: Journal article |
Palavras-chave: Ischemia; Estrogen; Muscle damage; Skeletal muscle; Neutrophils. |
| Ano: 2005 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000200011 |
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| Zhao,Liping; Yan,Wenhui; Xiang,Heng; Wang,Xiaoyang; Qiao,Haixuan. |
| The mechanisms of exercise-induced fatigue have not been investigated using proteomic techniques, an approach that could improve our understanding and generate novel information regarding the effects of exercise. In this study, the proteom alterations of rat skeletal muscle were investigated during exercise-induced fatigue. The proteins were extracted from the skeletal muscle of SD rat thigh, and then analyzed by two-dimensional electrophoresis and PDQuest software. Compared to control samples, 10 significantly altered proteins were found in exercise samples, two of them were upregulated and eight of them were downregulated. These proteins were identified by MALDI TOF-MS. The two upregulated proteins were identified as MLC1 and myosin L2 (DTNB) regulatory... |
| Tipo: Journal article |
Palavras-chave: Proteomics; Exercise-induced fatigue; Skeletal muscle; Rat. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602012000100010 |
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| Bosi,Paula Lima; Borges,Gabriel Delfino; Durigan,João Luiz Quagliotti; Cancelliero,Karina Maria; Silva,Carlos Alberto da. |
| The aim of this study was to evaluate the glycogen content (GC) of the rat hind limb muscles submitted to joint immobilization, either associated with metformin treatment (M, 1,4mg.ml-1) or not. In the metformin group, there was a significant increase in the GC (soleus - S 65% , white gastrocnemius - WG 30.5%, red gastrocnemius- RG31.7%, extensor digitorum longus - EDL 44%, tibialis anterior- TA 77.4%). The immobilization significantly reduced the GC (S 31.6%, WG 56.6%, RG 39.1%, ELD 41.7%, TA 45.2%) and weight (S 34.2% and ELD 27%), whereas in the group immobilized with the metformin, there was an increase in the GC of all the muscles (S 177%, WG 290%, RG 172%,ELD 47%, TA 217%), in addition to minimizing the weight loss of S (29.6%) and ELD (27.8%). |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Muscle disuse; Metformin; Skeletal muscle; Rehabilitation. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000200009 |
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| Silvestre,J.G.; Baptista,I.L.; Silva,W.J.; Cruz,A.; Silva,M.T.; Miyabara,E.H.; Labeit,S.; Moriscot,A.S.. |
| Fibroblasts are a highly heterogeneous population of cells, being found in a large number of different tissues. These cells produce the extracellular matrix, which is essential to preserve structural integrity of connective tissues. Fibroblasts are frequently engaged in migration and remodeling, exerting traction forces in the extracellular matrix, which is crucial for matrix deposition and wound healing. In addition, previous studies performed on primary myoblasts suggest that the E3 ligase MuRF2 might function as a cytoskeleton adaptor. Here, we hypothesized that MuRF2 also plays a functional role in skeletal muscle fibroblasts. We found that skeletal muscle fibroblasts express MuRF2 and its siRNA knock-down promoted decreased fibroblast migration, cell... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fibroblast; Skeletal muscle; Migration; MuRF2; E3 ligase; Wound healing. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019000900604 |
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| Vesentini,G.; Marini,G.; Piculo,F.; Damasceno,D.C.; Matheus,S.M.M.; Felisbino,S.L.; Calderon,I.M.P.; Hijaz,A.; Barbosa,A.M.P.; Rudge,M.V.C.. |
| The urethral muscle of diabetic pregnant rats is affected by long-term mild diabetes and short-term severe diabetes, which plays a crucial role in the pathogenesis of pelvic floor disorders. We hypothesized that muscles outside the pelvis are subject to similar changes. The current study aimed at analyzing the effects of long-term mild and short-term severe diabetes on the structure and ultrastructure of fiber muscles and collagen in rats' rectus abdominis (RA) muscle. Therefore, the RA muscle of virgin, pregnant, long-term mild diabetic, short-term severe diabetic, long-term mild diabetic pregnant and short-term severe diabetic pregnant 3-month-old Wistar rats were collected. The structure was analyzed by picrosirius red staining, immunohistochemistry for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diabetes; Pregnancy; Collagen; Skeletal muscle; Rats. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000400611 |
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| Registros recuperados: 40 | |
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