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| Zhang,Xiaoli; Han,Xiaoxu; Dai,Di; Mingjia,Bao; Zhang,Zining; Zhang,Min; Bice,Tristan; Zhao,Min; Cao,Yaming; Shang,Hong. |
| OBJECTIVE: One approach to identifying HIV-1 vaccine candidates is to dissect the natural antiviral immune response in treatment-naïve individuals infected for over ten years, considered slow progressor patients (SPs). It is suspected that SP plasma has strongly neutralizing antibodies (NAb) targeting specific HIV viral epitopes. METHODS: NAbs levels of 11 HIV-1-infected SPs were detected by PBMC-based neutralization assays. To investigate SP NAb epitope, this study used a biopanning approach to obtain mimotopes of HIV-1 that were recognized by SP plasma NAbs. IgG was purified from hightiter NAb SP plasma, and used as the ligand for three rounds of biopanning to select HIV-specific mimotopes from a phage-displayed random peptide library. Double-antibody... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: HIV-1 vaccine; Slow progressor; Neutralizing antibodies. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000600002 |
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