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	Provedor de dados BJMBR (2) Autor Cui,W. (1) Dai,Jinhua (1) Ge,Zhibin (1) Jin,H.B. (1) Li,Z.W. (1) Zhang,Haijun (1) Zheng,Xingguo (1) Mais... Palavra-chave Smad (2) Bone fracture (1) CAMP (1) Fibrogenesis (1) Fibronectin (1) Hepatic stem cell (1) MicroRNA-221 (1) Osteoblast differentiation (1) TGF-β (1) Wnt/Notch (1) ZFPM2 (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2018 (1) 2010 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Mechanism of the transforming growth factor-β induction of fibronectin expression in hepatic stem-like cells BJMBR Cui,W.; Jin,H.B.; Li,Z.W.. Transforming growth factor-β1 (TGF-β1) plays an important role in the fibrogenic process in the liver. The aim of the present study was to explore the action of TGF-β1 on fibronectin expression in rat hepatic stem-like cells and the underlying mechanisms. The level of fibronectin expression was determined in hepatic stem-like cells (WB cells) before and after TGF-β1 stimulation by RT-PCR and Western blot methods. Using immunogold transmission electron microscopy and the Western blot method, we observed the result of the expression and the distribution of cAMP, phosphorylated Smad3 and Smad7 before and after TGF-β1 treatment. The levels of fibronectin expression in both mRNA and protein increased 4- to 5-fold after TGF-β1 stimulation, reaching an optimum... Tipo: Info:eu-repo/semantics/article Palavras-chave: TGF-β; Fibronectin; Fibrogenesis; Hepatic stem cell; CAMP; Smad. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000100006 MicroRNA-221 promotes cell proliferation, migration, and differentiation by regulation of ZFPM2 in osteoblasts BJMBR Zheng,Xingguo; Dai,Jinhua; Zhang,Haijun; Ge,Zhibin. Bone fracture is a common medical condition, which may occur due to traumatic injury or disease-related conditions. Evidence suggests that microRNAs (miRNAs) can regulate osteoblast differentiation and function. In this study, we explored the effects and mechanism of miR-221 on the growth and migration of osteoblasts using MC3T3-E1 cells. The expression levels of miR-221 in the different groups were measured by qRT-PCR. Then, miR-221 mimic and inhibitor were transfected into MC3T3-E1 cells, and cell viability and migration were measured using the CCK-8 assay and the Transwell migration assay. Additionally, the expression levels of differentiation-related factors (Runx2 and Ocn) and ZFPM2 were measured by qRT-PCR. Western blot was used to measure the... Tipo: Info:eu-repo/semantics/article Palavras-chave: MicroRNA-221; Bone fracture; Osteoblast differentiation; ZFPM2; Wnt/Notch; Smad. Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018001200602 Registros recuperados: 2 Primeira ... 1 ... Última

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