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| Oliveira,R.J.; Mantovani,M.S.; da Silva,A.F.; Pesarini,J.R.; Mauro,M.O.; Ribeiro,L.R.. |
| The compounds 6-dimethylaminopurine and cycloheximide promote the successful production of cloned mammals and have been used in the development of embryos produced by somatic cell nuclear transfer. This study investigated the effects of 6-dimethylaminopurine and cycloheximide in vitro, using the thiazolyl blue tetrazolium bromide colorimetric assay to assess cytotoxicity, the trypan blue exclusion assay to assess cell viability, the comet assay to assess genotoxicity, and the micronucleus test with cytokinesis block to test mutagenicity. In addition, the comet assay and the micronucleus test were also performed on peripheral blood cells of 54 male Swiss mice, 35 g each, to assess the effects of the compounds in vivo. The results indicated that both... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Somatic cell nuclear transfer; 6-Dimethylaminopurine; Cycloheximide; Genotoxic; Mutagenic. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000400287 |
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| BHUIYAN, Mohammad Musharraf Uddin; SUZUKI, Yo; WATANABE, Hiroyuki; LEE, Eunsong; HIRAYAMA, Hiroki; MATSUOKA, Koji; FUJISE, Yoshihiro; ISHIKAWA, Hajime; OHSUMI, Seiji; FUKUI, Yutaka; 福井, 豊. |
| The objectives of this study were to choose an effective embryo reconstruction method and an effective post-activation agent for in vitro production of sei whale (Balaenoptera borealis) interspecies somatic cell nuclear transfer (iSCNT) embryos. Moreover, trichostatin A (TSA) treatment of whale iSCNT embryos was performed to improve the in vitro embryo development. In Experiment 1, the fusion rate was significantly higher (88.1%) in embryos reconstructed using the intracytoplasmic cell injection method (ICI) than that (48.7%) in the subzonal cell insertion (SUZI) counterpart. The rates of pseudopronucleus (PPN) formation (77.4 vs. 77.2%) and cleavage (24.5 vs. 37.0%) did not vary between ICI and SUZI. However, the PPN formation and cleavage rates were... |
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Palavras-chave: Interspecies; Somatic cell nuclear transfer; Whale. |
| Ano: 2010 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2815 |
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