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| Registros recuperados: 42 | |
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| GALLEGO RÚA,Adriana María; HENAO RAMÍREZ,Ana María; URREA TRUJILLO,Aura Inés; ATEHORTÚA GARCÉS,Lucía. |
| In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the regenerative capability of somatic embryos; thus, we followed histochemical changes and reserve fluctuations of proteins, polysaccharides and polyphenols during somatic embryogenesis (SE) in the two cacao varieties. The study showed that, in somatic embryos of the regenerating variety, polyphenols were localized mainly in the periphery of the embryo (epidermal cells) and proteins were the main storage substance in the embryo expression medium, while the non-regenerating... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidants; Histology; Reserve accumulation; Recalcitrance; Somatic embryogenesis. |
| Ano: 2016 |
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2016000200002 |
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| Texeira, Leandra Regina; UEM; Braccini, Alessandro De Lucca e; UEM; Churata, Bruno Grimaldo Martinho; UFPR; Vieira, Elisa Serra Negra; Cooperativa Central de Pesquisa Agrícola; Martins, Polyana Kelly; Cooperativa Central de Pesquisa Agrícola; Schuster, Ivan; Cooperativa Central de Pesquisa Agrícola. |
| The first step in plant transformation using somatic embryogenesis or organogenesis for plant regeneration in vitro has been the identification of genotypes with the best totipotency. Thus, the objective of this experiment was to evaluate the totipotency of ten cultivars and one soybean line from the Coodetec Gene Bank. The somatic embryos developed from immature cotyledons measuring from 4 to 6 mm in length, and the organogenesis was evaluated from mature soybean seeds. The cultivars CD 201, CD 216 and CD 220 were the most promising genotypes to produce somatic embryos, whereas the cultivar CD 217 had the highest quantity of differentiated explants, which represents the best regeneration efficiency for participating in organogenic systems. In the... |
| Tipo: Laboratory analysis |
Palavras-chave: Glycine max; Somatic embryogenesis; Organogenesis Glycine max; Somatic embryogenesis; Organogenesis Melhoramento Vegetal; Cultura de Tecidos. |
| Ano: 2011 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/12344 |
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| Rossato, Marieli; Schumacher, Pedro Vitor; Costa Netto, Antônio Paulino da; Stein, Vanessa Cristina; Reis, Edésio Fialho dos; Vilela, Marlúcia Souza Pádua; Paiva, Luciano Vilela. |
| Campomanesia adamantium is a native plant species of Brazilian Cerrado with diverse economic potential and great medicinal importance. Its sexual propagation is impaired by the recalcitrance of its seeds, which prevents effective and profitable propagation. With the purpose of establishing commercial crops and minimizing the extractive use of vegetal resources, the aim of the present study was to induce embryogenic calli in nodal segments of gabirobeira, and to determine and characterize their embryogenic phase through the establishment of a growth curve based on cellular characteristics. Calli were induced using nodal segments inoculated in WPM culture medium without the addition of hormones (control) and with different concentrations of 2,4-D, IAA, IBA,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Somatic embryogenesis; Growth regulator; Callus. |
| Ano: 2019 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/46358 |
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| Montero-Cortés,Mayra I.; Chan-Rodríguez,José L.; Cordova-Lara,Ivan; Oropeza-Salin,Carlos; Sáenz-Carbonell,Luis. |
| Coconut (Cocos nucífera L.) production has been declining in México and other countries due to deadly diseases; therefore, massive production of palms resistant to diseases is needed for replanting. Production of somatic embryos and micropropagation could be a very useful technique to produce free disease coconut population. Explants (embryogenic-structures) of Pacific Tall 2 ecotype were cultured in an induction medium defined as I (semisolid Y3 medium containing 0.65 mM 2,4-dichlorophenoxyacetic acid) and treated with the cytokinin benzyladenine (BA) at 0, 25, 100 or 200 ,µM to evaluate its effect on embryogenic callus formation. The BA treatments did not affect the percentage of embryogenic callus formation. In order to evaluate the effect of the BA... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cocos nucífera; Clonal propagation; Somatic embryogenesis; Cytokinin. |
| Ano: 2011 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952011000600002 |
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| Ahmadpour,Roghayeh; Zare,Nasser; Asghari-Zakarta,Rasool; Sheikhzadeh,Parisa. |
| ABSTRACT An efficient regeneration system is a pre-requisite for the application of genetic transformation and functional genomics study of important plants. In this study, the effect of different factors (plant growth regulators, casein hydrolysate, aspartic acid and ascorbic acid) on in vitro embryogenesis and regeneration of Arta, Bahar and Zagros cultivars from mature and immature explants were investigated. Immature and mature embryos were dissected from disinfected seeds 20-25 days after pollination and imbedded mature seeds, respectively, and cultured on MS (Murashige and Skoog) medium supplemented with different compounds. The results showed that immature embryos expose high capacity of embryogenesis and regeneration in comparison with mature... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: In vitro culture; Plant growth regulators; Somatic embryogenesis; Wheat. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100206 |
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| Takahashi,Elizabete Keiko; Kobayashi,Adilson Kenji; Vieira,Luiz Gonzaga Esteves. |
| The objective of this study was to examine the effect of two culture systems, liquid medium associated to floating membranes and solid medium, both supplemented with different concentrations of 2,4-D, in the induction of somatic embryogenesis of cassava (Manihot esculenta Crantz). Only 28% of the young leaf lobes (with 9 µM 2,4-D) were induced to form organized embryogenic structures (OES) with membrane rafts, compared to 50% of the explants presenting this type of tissue in solid medium with 36 µM of 2,4-D. Despite the lower response observed in liquid medium with membrane, the amount of OES/explant in all 2,4-D concentrations was higher than solid medium. Based on the results and considering the high cost of the membrane rafts, this system was not... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Manihot esculenta; Somatic embryogenesis; Membrane rafts; Organized embryogenic structures (OES). |
| Ano: 2000 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000100005 |
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| Bajpai,Anju; Kalim,Shahina; Chandra,Ramesh; Kamle,Madhu. |
| A simple and efficient protocol for recurrent somatic embryogenesis and plant regeneration is one of the prerequisites for genetic improvement of guava. An efficient reproducible regeneration somatic embryogenesis protocol was developed in four genotypes of Psidium guajava L. using immature zygotic embryo as starter explant. Somatic embryogenesis induction was obtained on MS basal medium supplemented with 2.0 mgL-1 2, 4-D, 400 mgL-1 L-glutamine, 6% sucrose and 500 mgL-1 Malt extract. Following SE induction different developmental stages of somatic embryos (Globular, heart-shaped, torpedo, cotyledonary) was directly obtained and further recurrent embryogenesis also obtained upon prolonged incubation in induction media. Addition of polyethylene glycol (50... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Somatic embryogenesis; Zygotic embryo; Recurrent; Maturation; Acclimatization; Induction; Regeneration. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100412 |
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| Santos,André Luis Wendt dos; Silveira,Vanildo; Steiner,Neusa; Vidor,Mário; Guerra,Miguel Pedro. |
| Embryogenic cultures of Araucaria angustifolia were induced from dominant and non-dominant zygotic embryos excised from immature seeds proceeding from three different genotypes and five harvest dates. Zygotic embryos were inoculated in inductive culture medium LP and BM supplemented with or without plant growth regulators 2,4-D (5 µM), BA (2 µM) and Kin (2 µM). The genotype of the mother tree and the developmental explant stage affected the induction frequency. In the maintenance phase, embryogenic cultures were maintained at continuous repetitive cell cycles every 20 days in semi-solid or liquid medium. In the maturation phase the culture medium was supplemented with different types and levels of growth regulators, osmotic agents, carbohydrates and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Araucaria angustifolia; Conifer; Somatic embryogenesis; Picea abies; Embryo maturation; Pinus sp.. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132002000100015 |
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| Etienne,Hervé; Dechamp,E; Barry-Etienne,D; Bertrand,Bernóit. |
| In coffee, bioreactors are the most promising way for scaling-up micropropagation processes, particularly somatic embryogenesis. The availability of an efficient somatic embryogenesis process would allow the rapid mass production of heterozygous materials such as selected Coffea canephora clones and F1 Arabica hybrid varieties. For the last fifteen years, bioreactors (mechanically or pneumatically agitated bioreactors, temporary immersion bioreactors) have mostly been used on coffee to optimize the mass regeneration of somatic embryos from embryogenic tissues. This review presents the main results, obtained with several bioreactor models, concerning the different steps of the micropropagation process : i) the multiplication of embryogenic tissues, ii) the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coffea; Breeding; Liquid medium; Mass propagation; Somatic embryogenesis; Temporary immersion. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100005 |
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| Cartes R,Priscila; Castellanos B,Hermes; Ríos L,Darcy; Sáez C,Katia; Spierccolli H,Scarlette; Sánchez O,Manuel. |
| Somatic and zygotic embryos from mature seeds of rauli-beech, Nothofagus alpina (Poepp. & Endl.) Oerst., were encapsulated in different artificial endosperms in order to generate a cover that fulfills the function of nourishment and protection of the embryos, facilitating their later germination. The content of sodium alginate varied by 4%, 3%, and 2%, as did the immersion time in calcium chloride (CaCl2), which acts as complexing agent. The artificial endosperm components of the Murashige and Skoog medium (MS) were added, supplemented with 0.5 mg L-1 indolacetic acid (IAA), 0.5 mg L-1 naphthaleneacetic acid (NAA), 2 mg L-1 6-benzylaminopurine (BAP) and 30 g L-1 sucrose. The germinative behaviors of encapsulated somatic and zygotic embryos were... |
| Tipo: Journal article |
Palavras-chave: Rauli-beech; Somatic embryogenesis; Synthetic seed; Sodium alginate. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000100014 |
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| Cartes Riquelme,Pricila; Ríos Leal,Darcy; Sáez Carrillo,Kátia; Uribe Moraga,Matilde; Valenzuela Aguilar,Sofía; Joseph Bolus,Stephen; Sánchez Olate,Manuel. |
| Abscisic acid (ABA) and indole-3-acetic acid (IAA) participate in the propagation of plants by somatic embryogenesis, causing polar structural differentiation of the embryo. The goal of the assay was to compare endogenous levels of ABA and IAA between somatic embryos (SE) and zygotic embryos (ZE) of Nothofagus alpina (Poepp. & Endl.) Oerst. In this study, a somatic embryo maturation assay involving the addition of varying concentrations of exogenous ABA was performed on cotyledonary-stage of N. alpina. Furthermore, the endogenous levels of ABA and IAA were quantified in the immature ZE, the mature ZE, and the embryonic axis of a mature embryo of N. alpina. The current study utilized high performance liquid chromatography (HPLC) for quantification.... |
| Tipo: Journal article |
Palavras-chave: Somatic embryogenesis; Maturation; Germination; HPLC. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392011000400007 |
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| Vázquez Cisneros, Isaac. |
| El linaloe es un árbol conocido por su aroma agradable; la madera se utiliza para fabricar artesanías, y los frutos para extracción de aceite esencial, sin embargo, su aprovechamiento ha sido descontrolado y no existe manejo forestal para esta especie, lo cual reduce la población natural. Se plantea mejorar la metodología de embriogénesis somática tradicional mediante un sistema de inmersión temporal, así mismo, evidenciar el proceso embriogénico indirecto y secundario, finalmente comprobar la producción de metabolitos secundarios de interés. Se utilizó la línea embriogénica LEL 61-4, y un medio de cultivo base MS complementado con 30 g L-1 sacarosa, 0.022 μM bencil adenina y 0.027 μM ácido naftalenacético, con temperatura de 25 ± 2 ºC y fotoperiodo de 16... |
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Palavras-chave: Linaloe; Burseraceae; Sistema de inmersión temporal; Calidad de luz; Embriogénesis somática; Temporary immersion system; Light type; Somatic embryogenesis; Forestal; Maestría. |
| Ano: 2014 |
URL: http://hdl.handle.net/10521/2236 |
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| Registros recuperados: 42 | |
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