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| Registros recuperados: 42 | |
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| Vázquez Cisneros, Isaac. |
| El linaloe es un árbol conocido por su aroma agradable; la madera se utiliza para fabricar artesanías, y los frutos para extracción de aceite esencial, sin embargo, su aprovechamiento ha sido descontrolado y no existe manejo forestal para esta especie, lo cual reduce la población natural. Se plantea mejorar la metodología de embriogénesis somática tradicional mediante un sistema de inmersión temporal, así mismo, evidenciar el proceso embriogénico indirecto y secundario, finalmente comprobar la producción de metabolitos secundarios de interés. Se utilizó la línea embriogénica LEL 61-4, y un medio de cultivo base MS complementado con 30 g L-1 sacarosa, 0.022 μM bencil adenina y 0.027 μM ácido naftalenacético, con temperatura de 25 ± 2 ºC y fotoperiodo de 16... |
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Palavras-chave: Linaloe; Burseraceae; Sistema de inmersión temporal; Calidad de luz; Embriogénesis somática; Temporary immersion system; Light type; Somatic embryogenesis; Forestal; Maestría. |
| Ano: 2014 |
URL: http://hdl.handle.net/10521/2236 |
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| Seyring,Martina; Ewald,Aloma; Mueller,Anke; Haensch,Klaus-Thomas. |
| In the present study we examined the possibility of propagating different Cyclamen species (C. africanum Boiss. and Reut., C. cilicium Boiss. and Heldr., C. coum Mill., C. hederifolium Ait., C. persicum Mill., C. purpurascens Mill.) including some of their subspecies and cultivars in vitro using explants of adult plants. For this purpose two protocols have been applied to eleven genotypes combined with mostly four explant types (placentas with ovules, leaves, petioles and peduncles). The use of these protocols has given rise to either somatic embryo-like structures and/or adventitious shoots in all genotypes. This way it was possible to propagate each of the examined genotypes in vitro using explants of adult plants in a time less than one year. These... |
| Tipo: Journal article |
Palavras-chave: Propagation; Shoot regeneration; Somatic embryogenesis; Species. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000400010 |
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| Haensch,Klaus-Thomas. |
| This long-term study demonstrates for the first time that it is possible to propagate embryogenic cultures in pelargoniums and to subsequently initiate the differentiation of embryos using the cultivar Madame Layal (Pelargonium x domesticum). Propagation of callus was only possible with combinations of 2,4-dichlorophenoxyacetic acid (2,4-D) and 6-benzylaminopurine (BAP), which gave rise to embryos from the primary culture stage on. However, the propagation of cells, as well as the differentiation of embryos, was inhibited by a continuous application of these growth regulators. For this reason, a long period on medium lacking growth regulators was necessary before the differentiation of embryos occurred again. The consequences for improving the propagation... |
| Tipo: Journal article |
Palavras-chave: Auxin; Cytokinin; Propagation; Somatic embryogenesis. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100007 |
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| González,Germán Andrés; Pacheco,María Gabriela; Oneto,Cecilia Décima; Etchart,Valeria J; Kandus,Mariana V; Salerno,Juan Carlos; Eyherabide,Guillermo; Presello,Daniel; Lewi,Dalia Marcela. |
| Somatic embryogenesis, which is still the method of choice for tissue culture, regeneration and transformation of maize, is largely considered highly genotype-dependent. The Hi II, a highly embryogenic genotype, has been extensively used in transformation protocols. However, this is not an inbred line; instead, it has a proportion of the undesirable A-188 background, and the progeny segregates for phenotypic characteristics and shows poor agronomic performance. In an effort to identify genotypes that combine a high somatic embryogenic response and good agronomic performance, we evaluated 48 advanced inbred lines developed at INTA. Callus development and somatic embryogenesis capacity were measured in 200 immature embryos per line. Embryogenic capacity [EC... |
| Tipo: Journal article |
Palavras-chave: Callus; Inbred lines; Maize; Molecular markers; Regeneration; Somatic embryogenesis. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100009 |
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| Kumar,Vinod; Parvatam,Giridhar; Ravishankar,Gokare Aswathanarayana. |
| The aim of this review is to critically analyze the role of silver nitrate (AgNO3) in modulating plant growth and development. In recent years, basic studies on ethylene regulation opened new vistas for applied research in the area of micro-propagation, somatic embryogenesis, in vitro flowering, growth promotion, fruit ripening, and sex expression. Silver nitrate has proved to be a very potent inhibitor of ethylene action and is widely used in plant tissue culture. Few properties of silver nitrate such as easy availability, solubility in water, specificity and stability make it very useful for various applications in exploiting plant growth regulation and morphogenesis in vivo and in vitro. Silver ion mediated responses seem to be involved in polyamines,... |
| Tipo: Journal article |
Palavras-chave: Calcium; Ethylene; Morphogenesis; Polyamines; Silver nitrate; Somatic embryogenesis. |
| Ano: 2009 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200008 |
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| Ma,Jun; He,Yehua; Hu,Zhongyi; Xu,Wentian; Xia,Jingxian; Guo,Cuihong; Lin,Shunquan; Chen,Chengjie; Wu,Chenghou; Zhang,Junli. |
| Two somatic embryogenesis receptor-like kinase genes (identified as AcSERK1 and AcSERK2) have previously been characterized from pineapple (Ananas comosus). In this work, we describe the characterization of a third gene (AcSERK3) in this family. AcSERK3 had all the characteristic domains and shared extensive sequence homology with other plant SERKs. AcSERK3 expression was studied by in situ hybridization and quantitative real-time PCR to analyze its function. Intense in situ hybridization signals were observed only in single competent cells and competent cell clusters; no hybridization signal was detected in the subsequent stages of somatic embryogenesis. AcSERK3 was highly expressed in embryogenic callus compared to other organs, e.g., 20-80 fold more... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: AcSERK3; Autophosphorylation activity; Expression analysis; Prokaryotic expression; Somatic embryogenesis. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000400009 |
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| Li,Hui-Liang; Guo,Dong; Zhu,Jia-Hong; Wang,Ying; Peng,Shi-Qing. |
| Abstract Histone methylation plays a crucial role in various biological processes, from heterochromatin formation to transcriptional regulation. Currently, no information is available regarding histone methylation modifiers in the important rubber-producing plant Hevea brasiliensis. Here, we identified 47 histone methyltransferase (HMT) genes and 25 histone demethylase (HDM) genes as possible members of the histone methylation modifiers in the rubber tree genome. According to the structural features of HMT and HDM, the HbHMTs were classified into two groups (HbPRMs and HbSDGs), the HbHDMs have two groups (HbLSDs and HbJMJs). Expression patterns were analyzed in five different tissues and at different phases of somatic embryogenesis. HbSDG10, 21, 25, 33,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Hevea brasiliensis; Histone methylation; Histone methyltransferase; Histone demethylase; Somatic embryogenesis. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572020000100301 |
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| Morais-Lino,Lucymeire Souza; Santos-Serejo,Janay Almeida dos; Silva,Sebastião de Oliveira e; Santana,José Raniere Ferreira de; Kobayashi,Adilson Kenji. |
| The objective of this study was to establish cell suspension culture and plant regeneration via somatic embryogenesis of a Brazilian plantain, cultivar Terra Maranhão, AAB. Immature male flowers were used as explant source for generating highly embryogenic cultures 45 days after inoculation, which were used for establishment of cell suspension culture and multiplication of secondary somatic embryos. Five semisolid culture media were tested for differentiation, maturation, somatic embryos germination and for plant regeneration. An average of 558 plants per one milliliter of 5% SCV (settled cell volume) were regenerated in the MS medium, with 11.4 µM indolacetic acid and 2.2 µM 6-benzylaminopurine. Regenerated plants showed a normal development, and no... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Musa; Culture media; Propagation; Somatic embryogenesis. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008001000010 |
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| SANTA-CATARINA,CLAUDETE; MACIEL,SCHEILA DA CONCEIÇÃO; PEDROTTI,ENIO LUIZ. |
| Embriões zigóticos em diferentes estádios de desenvolvimento foram utilizados para avaliar o potencial de germinação e competência embriogênica in vitro. Frutos coletados entre 15 e 360 dias após a antese foram avaliados em relação ao diâmetro (mm), comprimento (mm) e massa fresca (g). Para avaliar a percentagem de germinação in vitro, embriões zigóticos em diferentes estádios de desenvolvimento foram inoculados em meio de cultura composto de sais minerais de Murashigue & Skoog (MS), ou em meio MS suplementado com 4,4 miM de BAP, sacarose (3%), ágar (0,6%), carvão ativado (0,15%). Para a indução de embriogênese somática, eixos embrionários em diferentes estádios de desenvolvimento foram inoculados em meio de cultura MS, suplementado com 2,4-D (0,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Zygotic embryos; Somatic embryogenesis; In vitro germination; Woody plant; Lauraceae. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042001000500004 |
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| Costa,Adelaide de Fátima Santana da; Abreu,Emanuel Felipe Medeiros; Schmildt,Edilson Romais; Costa,Aureliano Nogueira da; Schmildt,Omar. |
| Abstract Papaya (Carica papaya L.) is a fruit tree of great economic and food interest worldwide. Its propagation can occur both sexually and asexually. Traditionally, it is propagated by seeds; however, vegetative propagation has been often used with the use of in vitro and ex vitro culture techniques. In vegetative propagation, the cutting method has been extensively explored. Research has also been developed using grafting. In recent years, papaya cultivation has also become a model for other fruit plants in the use of biotechnological techniques aiming applications ranging from germplasm conservation to genetic transformation of plants. Since the beginning of the 1970s, the culture of papaya tree tissues has been intensely studied, allowing greater... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carica papaya L.; Propagation of papaya; Vegetative propagation; Somatic embryogenesis. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000501001 |
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| Vidal,Ádila Melo; Costa,Maria Angélica Pereira de Carvalho; Souza,Antônio da Silva; Almeida,Weliton Antonio Bastos de; Souza,Fernanda Vidigal Duarte. |
| In this study, the somatic embryogenesis and regeneration of Cigana Preta plants from shoot apices and immature leaves taken from plants cultivated in vitro were examined. To embryo induction the explants were cultivated in Murashige and Skoog (MS) medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D) or 4-amino-3,5,6-trichloropicolinic acid (Picloram) at concentrations of 8.0 and 12 mg L-1. To development of embryos two media cultures with different concentration of BAP (D1 or D2) were evaluated. Embryos in the cotyledonary stage were incubated in germination medium containing MS salts and vitamins, 2.0 µM copper sulfate, 2.4 g L-1 of Phytagel® and 1.77 µM BAP. The highest frequency of calluses and the greatest number of embryos per explant were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cassava; Somatic embryogenesis; Regulators-plants. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902014000300017 |
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| Registros recuperados: 42 | |
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