|
|
|
|
|
| Pinheiro, Marcos Vinícius Marques; Universidade Federal de Viçosa; Martins, Fabrina Bolzan; Universidade Federal de Itajubá; Cruz, Ana Claudia Ferreira da; Universidade Federal de Viçosa; Carvalho, Ana Cristina Portugal Pinto de; Embrapa Agroindústria Tropical; Oliveira, Evelyn Jardim de; Universidade Federal de Viçosa; Otoni, Wagner Campos; Universidade Federal de Viçosa. |
| This study establishes a protocol for the induction of somatic embryogenesis in Anthurium andraeanum cv. Eidibel. The experiment was arranged in a completely randomized 5 x 5 x 5 factorial design using five explant types (whole leaves, half leaves; petiole; nodal segments and root segments) from in vitro plantlets; five auxins: indole-3-acetic acid (IAA), α-naphthaleneacetic acid (NAA), indole-3-butyric acid (IBA), 2,4-dichlorophenoxyacetic acid (2,4-D), and 4-amino-3,5,6-trichloropicolinic acid (Picloram); at five concentrations (0, 2.5, 5, 7.5 and 10 µM), with five replications using five Petri dishes. The cultures were maintained in a growth room at 25 ± 2ºC in the dark. The explant type was investigated for the induction of somatic embryogenesis in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: 5.00.00.00-4 auxins; Nodal segments; Somatic embryos Ciências Agrárias. |
| Ano: 2014 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/16557 |
| |
|
|
| Maciel, Simone Alencar; Universidade Federal do Acre; Fermino Junior, Paulo Cesar Poeta; Universidade Federal do Acre; Silva, Ricardo Alexandre; Universidade Estadual do Norte Fluminense Darcy Ribeiro; Scherwinski-Pereira, Jonny Everson; Embrapa Recursos Genéticos e Biotecnologia. |
| The objective of this study was to morpho-anatomically characterize nodular embryogenic calluses from zygotic embryos of peach palm during the induction of somatic embryogenesis. Immature zygotic embryos were pre-treated in MS medium added to Picloram and 2,4-D (25 µM) and BAP (0, 5, 10 µM). After three months, primary calluses were transferred to MS induction medium added to Picloram and 2,4-D (450 µM). After six months, the embryogenic calluses were then histologically analyzed and cultivated in the maturation medium. The competent tissues of the zygotic embryos differentiated embryogenic calluses under action of both Picloram and 2,4-D auxins (450 µM), where the presence of multi-granular structures were observed.... |
|
Palavras-chave: Bactris gasipaes; Micropropagation; Histology; Morphology; Callus anatomy; Somatic embryos 5.00.00.00-4 Bactris gasipaes; Micropropagation; Histology; Morphology; Callus anatomy; Somatic embryos Ciências Agrárias. |
| Ano: 2010 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/3248 |
| |
|
|
|
