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	Provedor de dados Electron. J. Biotechnol. (1) Genet. Mol. Biol. (1) Autor Chen,Fang (1) Herédia,Fabiana (1) Hu,Xiaole (1) Loreto,Elgion Lúcio S. (1) Valente,Vera Lúcia S. (1) Wei,Lei (1) Xu,Ying (1) Yin,Li (1) Mais... Palavra-chave Southern blot (2) Drosophila (1) Gypsy (1) Heterologous expression (1) PCR (1) Retrotransposon (1) Terpenoids (1) Transient expression (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2014 (1) 2007 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A novel chloroplastic isopentenyl diphosphate isomerase gene from Jatropha curcas: Cloning, characterization and subcellular localization Electron. J. Biotechnol. Wei,Lei; Yin,Li; Hu,Xiaole; Xu,Ying; Chen,Fang. Background Jatropha curcas is a rich reservoir of pharmaceutically active terpenoids. More than 25 terpenoids have been isolated from this plant, and their activities are anti-bacterial, anti-fungal, anti-cancer, insecticidal, rodenticidal, cytotoxic and molluscicidal. But not much is known about the pathway involved in the biosynthesis of terpenoids. The present investigation describes the cloning, characterization and subcellular localization of isopentenyl diphosphate isomerase (IPI) gene from J. curcas. IPI is one of the rate limiting enzymes in the biosynthesis of terpenoids, catalyzing the crucial interconversion of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP). Results A full-length JcIPI cDNA consisting of 1355 bp was cloned.... Tipo: Journal article Palavras-chave: Heterologous expression; Southern blot; Terpenoids; Transient expression. Ano: 2014 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600007 Distribution and conservation of the transposable element gypsy in drosophilid species Genet. Mol. Biol. Herédia,Fabiana; Loreto,Elgion Lúcio S.; Valente,Vera Lúcia S.. In an attempt to understand the dynamics of transposable elements (T'S) in the genome of host species, we investigated the distribution, representativeness and conservation of DNA sequences homologous to the Drosophila melanogaster gypsy retrotransposon in 42 drosophilid species. Our results extended the knowledge about the wide distribution of gypsy in the genus Drosophila, including several Neotropical species not previously studied. The gypsy-like sequences showed high divergence compared to the D. melanogaster gypsy element. Furthermore, the conservation of the restriction sites between gypsy sequences from phylogenetically unrelated species pointed to a more complex evolutionary picture, which includes the possibility of the horizontal transfer events... Tipo: Info:eu-repo/semantics/article Palavras-chave: Drosophila; Retrotransposon; Gypsy; Southern blot; PCR. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100023 Registros recuperados: 2 Primeira ... 1 ... Última

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