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Spectral fluorometric characterization of Haptophyte dynamics using the FluoroProbe: an application in the eastern English Channel for monitoring Phaeocystis globosa ArchiMer
Houliez, Emilie; Lizon, Fabrice; Thyssen, Melilotus; Artigas, Luis Felipe; Schmitt, Francois G..
In this study, we examined the possibility of using the FluoroProbe for monitoring the dynamics of the Haptophyte Phaeocystis globosa in the coastal waters of the eastern English Channel. The FluoroProbe was recalibrated by recording a new fingerprint for P. globosa and the use of this new fingerprint was tested through a series of laboratory and in situ experiments. The annual dynamics of P. globosa estimated using the FluoroProbe and by flow cytometry were similar. A strong relationship was found between the FluoroProbe estimates of P. globosa biomass expressed in terms of chlorophyll a equivalent per litre (eq. mu g L(-1)) and flow cytometric cell counts (r = 0.889, P < 0.001, n = 121). The FluoroProbe can be used to detect the flagellated cells as...
Tipo: Text Palavras-chave: Spectral fluorescence; Phaeocystis globosa; Haptophytes; Algal blooms; Phytoplankton quantification; Chlorophyll a.
Ano: 2012 URL: https://archimer.ifremer.fr/doc/00373/48461/48723.pdf
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Acquisition de données complémentaires aux dénombrements, avec les techniques de cytométrie en flux, fluorescence totale ou spectrale. Etat d’avancement et premiers résultats. Action Indice Composition. Livrable n° A III. Rapport final, 23 septembre 2014 ArchiMer
Artigas, Luis Felipe; Didry, Morgane; Barthelemy, Vanille; Bonato, Simon; Broutin, Mathias; Lizon, Fabrice; Lefebvre, Alain.
The European Union Water Framework Directive (WFD) refers to phytoplankton as one of the biological quality elements that should be regularly monitored and evaluated thanks to three ecological indices: biomass, abundance and composition. The first two have already been defined, but the third one has not yet been proposed in the English Channel-Atlantic zone. The construction of a composition indice must consider the diversity of all the phytoplankton. As the microscopic counts concern only the micro-phytoplankton, it is necessary to use additional information which concerns the nano- and pico-phytoplankton, of great importance when estimating the phytoplankton diversity. These components of the phytoplankton can be measured by different complementary...
Tipo: Text Palavras-chave: Manche Orientale; Groupes phytoplanctoniques; Cytométrie en Flux de type Scanning; Fluorimétrie spectrale; Analyses pigmentaires; Eastern English Channel; Phytoplankton groups; Scanning Flow Cytometry; Spectral fluorescence; Pigments analysis.
Ano: 2014 URL: http://archimer.ifremer.fr/doc/00361/47259/47224.pdf
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High resolution overview of phytoplankton spectral groups and hydrological conditions in the eastern English Channel using unsupervised clustering ArchiMer
Lefebvre, Alain; Poisson-caillault, Emilie.
As we move towards shipboard-underway and automated systems for monitoring water quality and assessing ecological status, there is a need to evaluate how effective the existing monitoring systems are, and how we could improve them. Considering the existing limitations for processing numerous and complex data series generated from automated systems, and because of processes involved in phytoplankton blooms, this paper proposes a data-driven evaluation of an unsupervised classifier to optimize the way we track phytoplankton, including harmful algal blooms (HABs), and to identify the main associated hydrological conditions. We used in situ data from a portable flow-through automatic measuring system coupled with a multi-fixed-wavelength fluorometer...
Tipo: Text Palavras-chave: Clustering; Spectral fluorescence; Phaeocystis globosa; English Channel Ferry Box; MSFD; Marine Strategy Framework Directive.
Ano: 2019 URL: https://archimer.ifremer.fr/doc/00479/59043/61793.pdf
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