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| Registros recuperados: 49 | |
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| Chatain, Beatrice; Poline, Olivier. |
| Objectives : Presently, most of marine fish farmers around the Mediterranean basin grow genetically wild fish strains, which have not been genetically improved for culture performance (growth, appearance, disease resistance etc.). It is inevitable that the present gap for genetically improved strains of marine fish, and their reliable supply, will be filled. It will ultimately dominate the competitive component of marine culture industries once they evolve into commodity-style agribusiness (with fish prices being set at the lowest possible cost of production and the best quality). Those countries, which can competitively develop and supply the improved strains, will enjoy the benefit (perhaps survival) for their local industries. They will avoid... |
| Tipo: Text |
Palavras-chave: Pathogen free; Genetic origin; Sperm; Cryopreservation; Sea bass. |
| Ano: 2005 |
URL: http://archimer.ifremer.fr/doc/2005/rapport-6494.pdf |
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| Suquet, Marc; Malo, Florent; Gourtay, Clemence; Ratiskol, Dominique; Riobe, Marion; Le Grand, Jacqueline; Queau, Isabelle; Mingant, Christian. |
| A basic cryopreservation protocol for great scallop sperm was recently published, with which thawed sperm survival remained limited to 25% of control values (Suquet et al. 2014). We designed here a set of five experiments to test ways of improving thawed sperm survival and storage by studying the effects of (1) cryoprotectant concentration; (2) sugar incorporation in the extender; (3) egg yolk addition to the extender; (4) sperm dilution rate; and (5) storage capacity of thawed sperm at 4 degrees C. A sixth experiment was then performed to compare rearing performances of larvae produced using thawed sperm from the optimal protocol defined in the present study with those produced using fresh sperm. During the first experiment, a significantly higher... |
| Tipo: Text |
Palavras-chave: Pecten maximus; Mollusc; Sperm; Cryopreservation; Larval rearing. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00359/47033/46946.pdf |
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| Barnabe, G.; Tournamille, J.-c.. |
| Mature eggs were expressed from captured female and fertilised artificially with slightly bloody sperm, in dry conditions or in a humid atmosphere, both at 13 degree C. The best incubation conditions proved to be 13 degree C, with streptomycin and penicillin. Another sample was kept at 18 degree C. Hatching occurred after 4 or 5 days, larvae showing positive phototropism. The system of renewal of water and artificial food is described. The embryonic reserve drop of lipid disappeared between day 14 and 16. Observations on larvae were like previous ones, but live larvae had larger mouths than had fixed specimens, and it was found that temperatures of 13 degree C or 18 degree C were suitable beyond the egg stage. A sudden change in temperature caused by a... |
| Tipo: Text |
Palavras-chave: Temperature; Sperm; Eggs; Dicentrarchus labrax; Artificial reproduction. |
| Ano: 1972 |
URL: http://archimer.ifremer.fr/doc/1972/publication-2063.pdf |
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| Abascal, F; Cosson, J; Fauvel, Christian. |
| Computer assisted sperm analysis (CASA) was used to characterize the motility of sea bass Dicentrarchus labrax spermatozoa and to study the effect of several physicochemical variables and heavy metals on sperm swimming performance. Duration of sperm motility in sea bass was very short (< 50 s). During the first 20 s all the motility variables measured remained approximately constant, the velocity and linearity of the movement being maximum during this period, while both variables decreased sharply later. While slight variations in pH did not significantly modify sperm swimming performance, changes in osmolality affected all the measured motility variables. Two of the heavy metals tested, Cu2+ and Pb2+, did not affect sperm motility when the activating... |
| Tipo: Text |
Palavras-chave: Sperm; Heavy metals; Dicentrarchus labrax; CASA. |
| Ano: 2007 |
URL: http://archimer.ifremer.fr/doc/2007/publication-4765.pdf |
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| Suquet, Marc; Pouvreau, Stephane; Queau, Isabelle; Boulais, Myrina; Le Grand, Jacqueline; Ratiskol, Dominique; Cosson, Jacky. |
| n brooding mollusc species that incubate larvae before their release in seawater, the biology of gametes remains poorly explored. The present study describes some biological characteristics of sperm of the European flat oyster, a native species that has been over-exploited in the past and is nowadays classified as an endangered species in Europe. Flat oysters were collected by divers in the Rade de Brest (Finistère, France), during its natural reproduction period. Gonadal pH is acidic (6.31 ± 0.10). Spermatozoa are clustered in spermatozeugmata, an acellular structure in which the sperm heads are embedded. After their transfer in seawater, spermatozeugmata have a mean diameter of 64 ± 3 μm and they release free spermatozoa for a mean duration of... |
| Tipo: Text |
Palavras-chave: Oyster; Ostrea edulis; Brooding species; Sperm; Spermatozeugmata; Endangered species. |
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00454/56539/58245.pdf |
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| Suquet, Marc; Dreanno, Catherine; Petton, Bruno; Normant, Yvon; Omnes, Marie-helene; Billard, Roland. |
| The survival of turbot eggs and the rearing capacities of larvae stemmed from artificial fertilization practices using frozen-thawed spermatozoa were evaluated. Furthermore, the viability of sperm samples stored during a 9 month period in liquid nitrogen was assessed. No significant difference in the fertilization rate, hatching rate, survival and wet weight of 10-day old larvae were observed using fresh or frozen-thawed spermatozoa. The motility recorded at 10 s and 60 s post-activation and the fertilization capacity of frozen-thawed spermatozoa were not significantly decreased during a 9 month storage period in liquid nitrogen. These results confirm the high quality of the turbot spermatozoa stemmed from the cryopreservation process, allowing their use... |
| Tipo: Text |
Palavras-chave: Turbot; Psetta maxima; Sperm; Cryopreservation; Hatching. |
| Ano: 1998 |
URL: http://archimer.ifremer.fr/doc/00176/28721/27176.pdf |
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| Rouxel, Catherine; Suquet, Marc; Cosson, J; Severe, Armelle; Quemener, Loic; Fauvel, Christian. |
| The biology of cod reproduction is well described in the scientific literature. However, sperm biology and spermatozoa management are poorly studied in this species. Because of its recent farming expansion, a better knowledge of cod gametes is becoming especially useful. This work aimed at establishing tools to study sperm biology in cod, and also investigated the existence of changes in cod sperm quality during the spawning period. We showed that sperm concentration could be assessed using spectrophotometry at 260 nm. Sperm motility significantly decreased after a 168-h storage at 4 degrees C. A 1:9 dilution of sperm in a non-activating medium (1/3 seawater and 2/3 freshwater, osmotic pressure: 360 mOsm kg(-1)) improved sperm storage. Sperm concentration,... |
| Tipo: Text |
Palavras-chave: Spermatozoa; Quality; Ageing; Sperm; Cod; Gadus morhua. |
| Ano: 2008 |
URL: http://archimer.ifremer.fr/doc/2008/publication-3829.pdf |
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| Suquet, Marc; Gourtay, Clemence; Donval, Anne; Le Goic, Nelly; Quere, Claudie; Malo, Florent; Le Grand, Jacqueline; Ratiskol, Dominique; Mingant, Christian; Fauvel, Christian. |
| Most publications devoted to the cryopreservation of mollusc sperm have focused on the definition of technical protocols, avoiding the description of sperm quality after thawing. The present study investigated the effects of cryopreservation on sperm quality in the great scallop. Wild scallop were fished during the natural spawning period and conditioned in the hatchery before use. Sperm samples were obtained after intragonadal injection of serotonin and cryopreserved using a previously published protocol. Sperm quality was assessed using a panel of four parameters: sperm motility characteristics, using a a computer assisted sperm analysis plugin with Image J, intracellular ATP content using an ATP-Lite kit, sperm integrity, using flow cytometry and sperm... |
| Tipo: Text |
Palavras-chave: Pecten maximus; Reproduction; Gamete; Sperm; Cryopreservation. |
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00317/42862/43912.pdf |
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| Lahaye, Jacqueline. |
| Representative species of the families Pleuronectidae, Bothidae and Soleidae were studied throughout the year. Changes are described which were seen in the ovaries, and photographs of cross sections of the tissue are shown. The progress of egg release was noted, and in some cases correlated studies were made of gonadal changes in male. The period of egg release was prolonged over 1 one half -2 months for plaice and turbot, and for 6 months for sole. Individual fish showed these extended periods of laying, and the actual months of the year differed for each species. The massive mobilisation of reserves which necessarily accompanied the release of large numbers of eggs was reflected by turbot, in sperm production. In addition, the increase in gonadosomatic... |
| Tipo: Text |
Palavras-chave: Gonadosomatic activity; Sperm; Species; Brittany coasts; Flat fishes; Sexual cycles. |
| Ano: 1972 |
URL: http://archimer.ifremer.fr/doc/1972/publication-2064.pdf |
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| Dorange, G; Lepennec, M. |
| The spermatozoon of Pecten maximus is of the primitive type usually associated with species having external fertilization or emitting the sperm in the water. The size of the nucleus is reduced. The middle piece is similar to that reported for several Bivalves. It contains glycogen. But the spermatozoon of P. maximus exhibits its own ultrastructural characteristics: the short acrosome differs in size, shape, position and ultrastructure or by a combination of these from that of many bivalves. No axial rods was observed in contrast to several species of Mytilidae, Veneridae and Ostreidae. These originalities could go to the genus level. |
| Tipo: Text |
Palavras-chave: Pecten maximus; Bivalvia; Ultrastructure; Sperm. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-1725.pdf |
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| Riesco, Marta F.; Felix, Francisca; Matias, Domitilia; Joaquim, Sandra; Suquet, Marc; Cabrita, Elsa. |
| Sperm cryopreservation is a widely employed technique that promotes alternative techniques to contribute to broodstock management or restoration programs for species of commercial interest, endangered species or species with an interesting genotype. The preservation of genetic material from improved stocks or from the original population is extremely important for the oyster aquaculture industry to prevent the potential impacts of epidemic diseases and natural disasters. The Portuguese oyster, Crassostrea angulata, was the most important species commercialized by the shellfish industry. However, inadequate management of this industry and pathology occurrences resulted in a significant decrease in natural populations. For this reason, in this work a... |
| Tipo: Text |
Palavras-chave: Crassostrea angulata; Sperm; Cryopreservation; Motility; Viability; Fertilization. |
| Ano: 2017 |
URL: http://archimer.ifremer.fr/doc/00333/44432/44098.pdf |
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| Suquet, Marc; Labbe, C.; Brizard, Raphael; Donval, Anne; Le Coz, Jean-rene; Quere, Claudie; Haffray, P.. |
| Changes in sperm features during the movement phase are especially interesting to study in external fertilization species whose sperm duration movement is long because this implies a significant adaptation of moving cells to the external medium. This study describes the changes in tetraploid Pacific oyster sperm characteristics in relation to time post activation. Sperm individually collected on three tetraploid males were activated in seawater. Their features were analysed over a 24 h period and compared to a sperm pool collected on three diploid males as a reference. The percentage of motile spermatozoa, the intracellular ATP content, and the fine structure of spermatozoa were studied in relation to time post activation. Furthermore, the fertilisation... |
| Tipo: Text |
Palavras-chave: Crassostrea gigas; Sperm; Tetraploid; Motility; ATP; Ultrastructure. |
| Ano: 2010 |
URL: http://archimer.ifremer.fr/doc/00006/11756/8639.pdf |
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| Mahabadi J,Amini; Khodayari,M; Bafrani H,Hassani; Nikzad,H; Taherian,A. |
| Sesamin is a major lignan constituent of sesame seed and considered as a key factor in a number of beneficial effects on human health. Sesame leaves intake improve and increase epididymal spermatocytes reserve in adult male sprague dawley rat. The present study was aimed to investigate the effects of contain sesame seed on epididymis histologhical structure. Thirty adult male rats were divided into two groups of 15 rats each. The regimen group received diet containing 30% sesame seed, while the control group received diet along 12 weeks. The right epididymis were removed and minced into several pieces on a specimen bottle containing normal saline for some few minutes to allow the sperms to become motile and swim out and sperm parameters were analyzed. Left... |
| Tipo: Journal article |
Palavras-chave: Diet; Epididymis; Sesame seed; Sperm; Hormone. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000100042 |
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| Mahabadi,Javad Amini; Bafrani,Hassan Hassani; Nikzad,Hossein. |
| Infertility is a great concern among the people of reproductive age. The use of natural products obtained from traditional herbs is appealing. Studies show that antioxidants are important in improving male infertility. Thirty adult male rats were randomly divided into two regimen and control groups. The regimen group received diet containing 30 % sesame seed, while the control group received standard diet for 12 weeks. Histology of prostate and seminal vesicle were evaluated and serum levels of FSH, LH and testosterone concentrations were assessed as well. The results showed that, the diameter of peripheral epithelium and the volume density of the prostate epithelium increased but the volume density of the prostate stroma was decreased significantly in the... |
| Tipo: Journal article |
Palavras-chave: Prostate; Seminal vesicle; Sesame seed; Sperm; Hormone. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200030 |
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| Bustos-Obregón,Eduardo; Poblete,Daniel; Catriao,Roberto; del Sol,Mariano; Fernandes,Fábio Henrique. |
| We evaluated the sperm parameters such as cauda epididymis weight, sperm count, sperm morphology and sperm DNA stability of adult CF-1 male mice treated daily (oral exposure) with the toxic sodium arsenite (As, 7.0 mg/kg/body weight); Melatonin (Me, 10.0 mg/kg/bw), Me (10.0 mg/kg/bw) plus As (7.0 mg/kg/bw) and Negative Control (NaCl 0.9%) to assess acute (8.3 days), chronic (33.2 days) and recovery of testicular damage (66.4 days). Arsenic decreases the number of sperm from chronic treatment (33.2 days) and this effect continued until 66.4 days of treatment. The toxic effect of As also altered the morphology of spermatozoa in all treatment periods when compared to the negative control group. However, Metalonin induced protective effects in periods of 33.2... |
| Tipo: Journal article |
Palavras-chave: Mouse; Melatonin; Arsenic; Sperm; DNA stability. |
| Ano: 2013 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022013000400017 |
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| Registros recuperados: 49 | |
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