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| Registros recuperados: 49 | |
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| Ansa, Anietie Archibong; Akpere, Odamesan; Imasuen, James Atekha. |
| This study evaluated the effect of Cd on some male reproductive parameters; and explored the therapeutic potentials of methanolic extract of Phoenix dactylifera (MEPD) fruit in averting such reproductive damages. 45 rabbit bucks aged 24-28 weeks and weighing 1.41-1.43 kg was used. The rabbits were assigned to 5 treatment groups (control, Cd-only, Cd + 300 mg MEPD, Cd + 600 mg MEPD and Cd + 900 mg MEPD) in a completely randomized design. The rabbits were dosed with 3 mg CdCl2 kg-1feed for 7 days followed by MEPD for 56 days after every 72 hours. Result of semen evaluation indicates that semen volume, motility, libido, concentration, total ejaculate, viability and morphology were significantly reduced by Cd compared to the normal control group. Testis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Animal Science Animal physiology and reproduction testis; Sperm; Heavy metal; Methanol; Plant extract.. |
| Ano: 2017 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/32858 |
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| SANCHES,EDUARDO G.; OLIVEIRA,IDILI R.; SERRALHEIRO,PEDRO C. DA SILVA; CERQUEIRA,VINICIUS R.. |
| This study aimed to develop a protocol of semen cryopreservation of the mutton snapper Lutjanus analis. The interaction between three extenders ( pH 6.1; 7.8 and 8.2) , two concentrations of dimethyl sulfoxide ( DMSO, 5 and 10%) and three cooling rates ( -90; -60 and -30°C.min−1) on the sperm motility rate and motility time were analyzed by a factorial experiment. A sample of 30 fishes ( 1,261 ± 449 g) collected in the nature was kept in floating net cages. The semen was frozen by using cryogenic straws, in nitrogen vapour and transferred, later, to liquid nitrogen. Fertilization test was accomplished to evaluate the viability of the cryopreserved sperm. The highest sperm motility rate and motility time ( P < 0.05) was achieved by combining extender C (... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryoprotector; Extender; Lutjanus analis; Reproduction; Sperm. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652013000301083 |
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| Souza,C.E.A.; Moura,A.A.; Lima-Souza,A.C.; Killian,G.J.. |
| The present study was designed to investigate the topographical distribution of seminal plasma (SP) proteins on epididymal and ejaculated bovine sperm. Using immunocytochemistry and confocal microscopy the binding patterns of bovine SP proteins BSP-A3, albumin, transferrin, prostaglandin D-synthase (PGDS) and nucleobindin in ejaculated and cauda epididymal sperm from adult bulls were evaluated. Experiments were performed using sperm from 5 males. Data showed a positive signal, only detected for anti-PGDS, in the acrosomal cap of epididymal and ejaculated sperm. In ejaculated sperm, a very weak signal for nucleobindin 2 in the midpiece and equatorial regions was detected, using the anti-rat nucleobindin. BSP-A3 was detected on all sperm regions studied,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Sperm; Seminal plasma proteins; Immunocytochemistry. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352011000300001 |
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| Martins,V.E.D.; Pinto,S.C.C.; Chaves,R.M.; Barros Filho,A.K.D.; Laskoski,L.M.; Souza,F.A.. |
| ABSTRACT This study aimed to evaluate the addition of Vitamin C, reduced Glutathione and trolox on sperm characteristics of pork refrigerated semen. Six pigs were collected through the technique of gloved hand (10 ejaculates/animals). The semen was diluted in MR-A®. After the previous evaluations, the treatments were added: Control group: diluent only; Vitamin C Group: 200μM/mL Vitamin C; Trolox Group: 200μM/mL Trolox; Glutathione group: 2.5mM/ml Reduced glutathione. The semen was stored in thermal boxes and placed inside the refrigerator at 15oC and evaluated at D0, 12, 48, 72 hours. After 30 hours of incubation, each treatment was divided into two equal fractions and the same concentration of antioxidants was added in one of the parts. The results show... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Glutathione; Sperm; Trolox; Vitamin C. |
| Ano: 2020 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100145 |
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| Ferreira,Adelina; Dolder,Heidi. |
| Spermatogenesis, with emphasis on spermiogenesis, is described for the lizard, Tropidurus itambere, using light microscopy, phase contrast and epifluorescence, as well as scanning and transmission electron microscopy. Cellular differentiation involves events of chromatin condensation, nuclear elongation and the formation of structural complexes, such as the acrosomal and axonemal ones. Other new characteristics, exclusive for this species, include various aspects of the subacrosomal granule, the insertion of the pro-acrosomal vesicle and the development of these structures to participate in the acrosomal complex. Radial projections occur just above the nuclear shoulders, which have been recognized already from the beginning of cellular elongation. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Ultrastructure; Sperm; Spermatogenesis; Lizard; Tropiduridae. |
| Ano: 2003 |
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452003000300006 |
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| Moreno,Ricardo D; Laserre A,Andrea; Barros,Claudio. |
| The interaction between acrosome-reacted sperm and zona pellucida proteins is not yet fully understood. Serine protease acrosin and its zymogen proacrosin have been proposed to fulfill this function due to their capacity to bind zona pellucida glycoproteins. However, the molecular mechanism underlying this interaction has been merely speculative. Here we show that fucoidan (a sulfated polysaccharide) and solubilized zona pellucida glycoproteins, but not soybean trypsin inhibitor, are able to detach bound spermatozoa, which suggests that live sperm binds to the zona pellucida in a non-enzymatical way. Interestingly, mild proteolytic digestion with acrosin or trypsin does not modify the structure of the zona pellucida, but rather results in fewer spermatozoa... |
| Tipo: Journal article |
Palavras-chave: Fertilization; Acrosin; Acrosome; Zona pellucida; Sperm. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200006 |
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| Cohen,Débora J; Maldera,Julieta A; Weigel Muñoz,Mariana; Ernesto,Juan I; Vasen,Gustavo; Cuasnicu,Patricia S. |
| Epididymal protein CRISPI is a member of the CRISP (Cysteine-RIch Secretory proteins) family and is involved in sperm-egg fusion through its interaction with complementary sites on the egg surface. Results from our laboratory have shown that this binding ability resides in a 12-amino-acid region corresponding to a highly conserved motif of the CRISP family, named Signature 2 (S2). In addition to this, our results revealed that CRISP1 could also be involved in the previous step of sperm binding to the zona pellucida, identifying a novel role for this protein in fertilization. As another approach to elucidate the participation of CRISP1 in fertilization, a mouse line containing a targeted disruption of CRISP1 was generated. Although CRISP1-deficient mice... |
| Tipo: Journal article |
Palavras-chave: Sperm; Gamete fusion; CRISP; Egg; Fertilization. |
| Ano: 2011 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000200004 |
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| Bertol,Melina Andrea Formighieri; Weiss,Romildo Romualdo; Thomaz-Soccol,Vanete; Kozicki,Luiz Ernandes; Fujita,Aline Silva; Abreu,Renata Azevedo de; Green,Kerriel Thandile. |
| The aim of this study was to evaluate the viability of bull spermatozoa collected from the cauda epididymis stored at 18-20°C, which were compared with semen collected by electro-ejaculation method and preserved at 5°C. Ten pairs of testes from Tabapuã bulls were removed by orchiectomy and stored for 6 (G6), 12 (G12), 18 (G18), 24 (G24) and 30 (G30) h at room temperature (18-20°C). Seven days before orchiectomy, semen was collected by electro-ejaculation method. The sperm parameters evaluated were: sperm motility, vigor, concentration, morphology and acrosome defects. Sperm motility declined (p<0.05) when spermatozoa were stored for 30 h in the epididymis. The spermatozoa from the epididymis showed lower sperm motility than that of spermatozoa collected... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bull; Epididymis; Orchiectomy; Sperm; Room temperature; Viability. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000500008 |
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| Tsai,S.; Lin,C.. |
| Cryopreservation is a long-term storage technique to preserve the biological material without deterioration for extended period of time at least several thousands of years. The ability to preserve and store both maternal and paternal gametes provides a reliable source of fish genetic material for scientific and aquaculture purposes as well as for conservation of biodiversity. Successful cryopreservation of fish sperm have been achieved for more than 200 fish species and many fish species have been adequated for the purpose of cryobanking. Cryopreservation of fish embryo is not viable, mainly because of the same limitations as in fish oocytes, i.e., high chilling sensitivity and low membrane permeability. However, cryopreservation of isolated embryonic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryopreservation; Sperm; Embryo; Oocyte; Blastomere. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000300014 |
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| Nowrouzi,Fatemeh; Azadbakht,Mehri; Kalehoei,Eshrat; Modarresi,Masoud. |
| Abstract The present study was aimed to investigate the in vivo effects of Rosa canina extract on doxorubicin-induced testicular toxicity in mice for the first time. Male NMRI mice were randomly divided into six treatment groups (10=per group) as follows: (I) vehicles, (II) doxorubicin alone (3 mg/kg, i.p. on days 7, 14 and 21), (III and IV): Rosa canina extract alone (100 mg/kg and 200 mg/kg per day, i.p. for 28 days), (V and VI) Rosa canina extract plus doxorubicin (each dose given 1 h post Rosa canina). Doxorubicin-treated mice displayed smaller body and testicular weights, decreased serum levels of testosterone, loss in the number of germ cells and Sertoli cells, and reduced sperm count, viability, morphology and motility. Doxorubicin treatment... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Rosa canina extract; Doxorubicin; Testes; Sperm; Mice. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100403 |
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| Chioca,Lea Rosa; Müller,Juliane Centeno; Boareto,Ana Cláudia; Andreatini,Roberto; Dalsenter,Paulo Roberto. |
| The aim of the present study was to evaluate the effects of sodium fluoride (NaF) on the male reproductive system. Adult male rats were exposed to NaF in drinking water for 30 days at three concentrations: 1.54 (control, tap water), 50 and 100 ppm. Body and organ weights, daily sperm production, sperm number and morphology were investigated. No difference was observed on the sperm number and morphology among the groups, as well as body weight and organ absolute and relative weights. Overall, despite the presence of a mild degree of dental fluorosis in the higher dose group, the results indicated that exposure to NaF at the doses used in the present study did not adversely affect sperm production and morphology of male rats. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Fluoride; Sodium fluoride; Male rats; Reproductive toxicology; Dental fluorosis; Sperm. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000200011 |
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| Passos,E.P.; Brugnara,L.; Facin,A.C.; Riffel,A.; Lima,G.R.; Freitas,V.; Brandelli,A.. |
| The effect of peritoneal fluid (PF) on the human sperm acrosome reaction (AR) was tested. Sperm was pre-incubated with PF and the AR was induced by calcium ionophore A23187 and a neoglycoprotein bearing N-acetylglycosamine residues (NGP). The AR induced by calcium ionophore was inhibited 40% by PF from controls (PFc) and 50% by PF from the endometriosis (PFe) group, but not by PF from infertile patients without endometriosis (PFi). No significant differences were found in the spontaneous AR. When the AR was induced by NGP, pre-incubation with PFc reduced (60%) the percentage of AR, while PFe and PFi caused no significant differences. The average rates of acrosome reactions obtained in control, NGP- and ionophore-treated sperm showed that NGP-induced... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Acrosome reaction; Endometriosis; Infertility; Peritoneal fluid; Sperm. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000100009 |
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| Moraes,A.J.P.; Pereira,R.M.R.; Cocuzza,M.; Casemiro,R.; Saito,O.; Silva,C.A.A.. |
| The objective of the present study was to identify sperm abnormalities in young male patients with juvenile dermatomyositis (JDM). In 2005, 18 male JDM patients, diagnosed according to the criteria of Bohan and Peter, were followed at the Pediatric Rheumatology Unit and Rheumatology Division, of our Institution. Of the 18 males, 11 were pre-pubertal and 7 were post-pubertal. Two of 7 post-pubertal JDM male patients were excluded: one for orchidopexy for cryptorchidism and the other for testicular ectopia in the left testis. The remaining 5 post-pubertal JDM patients were prospectively evaluated on the basis of two semen analyses, according to the World Health Organization (WHO), urologic evaluation, testicular Doppler ultrasound hormone profile. The data... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Juvenile dermatomyositis; Gonad; Sperm; Hormone; Male post-puberty; Idiopathic inflammatory myopathy. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2008001200016 |
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| Dorange, G; Lepennec, M. |
| The spermatozoon of Pecten maximus is of the primitive type usually associated with species having external fertilization or emitting the sperm in the water. The size of the nucleus is reduced. The middle piece is similar to that reported for several Bivalves. It contains glycogen. But the spermatozoon of P. maximus exhibits its own ultrastructural characteristics: the short acrosome differs in size, shape, position and ultrastructure or by a combination of these from that of many bivalves. No axial rods was observed in contrast to several species of Mytilidae, Veneridae and Ostreidae. These originalities could go to the genus level. |
| Tipo: Text |
Palavras-chave: Pecten maximus; Bivalvia; Ultrastructure; Sperm. |
| Ano: 1991 |
URL: http://archimer.ifremer.fr/doc/1991/acte-1725.pdf |
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| Barnabe, G.; Tournamille, J.-c.. |
| Mature eggs were expressed from captured female and fertilised artificially with slightly bloody sperm, in dry conditions or in a humid atmosphere, both at 13 degree C. The best incubation conditions proved to be 13 degree C, with streptomycin and penicillin. Another sample was kept at 18 degree C. Hatching occurred after 4 or 5 days, larvae showing positive phototropism. The system of renewal of water and artificial food is described. The embryonic reserve drop of lipid disappeared between day 14 and 16. Observations on larvae were like previous ones, but live larvae had larger mouths than had fixed specimens, and it was found that temperatures of 13 degree C or 18 degree C were suitable beyond the egg stage. A sudden change in temperature caused by a... |
| Tipo: Text |
Palavras-chave: Temperature; Sperm; Eggs; Dicentrarchus labrax; Artificial reproduction. |
| Ano: 1972 |
URL: http://archimer.ifremer.fr/doc/1972/publication-2063.pdf |
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| Registros recuperados: 49 | |
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