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| Registros recuperados: 51 | |
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| Narayan,Gopeshwar; Raman,Rajiva. |
| Eukaryotic chromatin is organized as radial DNA loops with periodical attachments to an underlying nucleoskeleton known as nuclear matrix. This higher order chromatin organization is revealed upon high salt extraction of cells. To understand the sequential change in the functional organization of chromatin during spermatogenesis, we have analysed the higher order organization of chromatin in different testicular cell types and the epididymal sperm of laboratory mouse. The expansion and contraction of the nucleoid DNA following 2 M NaCl extraction was measured in a fluorescence microscope using ethidium bromide (2.5-200 mg/mL) as an intercalating dye to induce DNA positive supercoils. While the halo size varied among cell types (pachytene DNA most extended,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromatin; DNase I; Nucleoid; Spermatogenesis. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000100006 |
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| Castanhole,Márcia Maria Urbanin; Pereira,Luis Lênin Vicente; Souza,Hederson Vinicius de; Valério,José Raul; Barbosa,Leonardo Rodrigues; Itoyama,Mary Massumi. |
| Spittlebugs annually infest pastures and cause severe damage, representing a serious problem for the tropical American beef cattle industry. Spittlebugs are an important biotic constraint to forage production and there is a lack of cytogenetic data for this group of insects. For these reasons, we conducted this work, in which the spermatogenesis and nucleolar behavior of Deois flavopicta, Mahanarva fimbriolata and Notozulia entreriana were studied. The males possessed testes in the shape of a "bunch of grapes"; a variable number of testicular lobes per individual and polyploid nuclei composed of several heteropycnotic bodies. A heteropycnotic area was located in the periphery of the nucleus (prophase I); the chiasmata were terminal or interstitial;... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Chromosomal characterization; Karyotype; Cytogenetics; Spermatogenesis; Holocentric chromosomes. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200008 |
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| Liu,Yunqiang; Tao,Dachang; Lu,Yongjie; Yang,Yuan; Ma,Yongxin; Zhang,Sizhong. |
| The mouse testis-enriched Znf230 gene, which encodes a type of RING finger protein, is present primarily in the nuclei of spermatogonia, the acrosome and the tail of spermatozoa. To investigate the role of Znf230 in spermatogenesis, we generated Znf230-deficient mice by disrupting Znf230 exon-5 and exon-6 using homologous recombination. The homozygous Znf230-knockout (KO) mice did not exhibit Znf230 mRNA expression and Znf230 protein production. Znf230 KO mice exhibited no obvious impairment in body growth or fertility. Male Znf230 KO mice had integral reproductive systems and mature sperm that were regular in number and shape. The developmental stages of male germ cells of Znf230 KO mice were also normal. We further examined variations in the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Znf230; Knockout mice; Spermatogenesis; Kctd14. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000500014 |
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| Moya,Ana C.; Andrade,María C. Díaz; Galíndez,Elena J.. |
| ABSTRACT The smallnose fanskate, Sympterygia bonapartii Müller & Henle, 1841 is one of the most disembarked items in commercial harbors in Argentina. In this work, the microscopic architecture of mature male gonads and the dynamics of cysts development are analyzed as a contribution to awareness of the reproductive biology of the species. Some biological data related to reproduction are given as well. Two seasons were sampled (fall and spring) and length classes's frequency distribution and maturity stages frequency distribution are given. Size at first sexual maturation for males was estimated at 57 cm of total length. Testes are symmetric, peer, lobed, with several germinal zones. Inside the gonads, there are many spermatocysts, containing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Histology; Testis; Smallnose fanskate; Spermatogenesis. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212015000300316 |
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| Schiavone, R; Zilli, L; Vilella, S; Fauvel, Christian. |
| The aims of the present study were (a) to compare sperm quality (percentage of motile spermatozoa, motility duration, density and fertility after cryopreservation) between precocious and normally maturing male European sea bass Dicentrarchus labrax, (b) to examine the potential of human chorionic gonadotropin (hCG) to increase spermiation in precocious males and (c) to examine the potential of hCG to induce spermatogenesis and spermiation in non-precocious 1-year-old males. One hundred precocious and 100 non-precocious fish were each randomly divided in two groups each: control (precocious saline-treated and non precocious saline-treated) and treated (precocious hCG-treated and non precocious hCG-treated). Treated groups were administered weekly with 1000... |
| Tipo: Text |
Palavras-chave: Dicentrarchus labrax; HCG; European sea bass; Spermiation; Spermatogenesis. |
| Ano: 2006 |
URL: http://archimer.ifremer.fr/doc/2006/publication-1815.pdf |
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| Avarre, Jean-christophe; Dugue, R.; Alonso, Pascal; Diombokho, A.; Joffrois, C.; Faivre, N.; Cochet, C.; Durand, Jean Dominique. |
| The black-chinned tilapia Sarotherodon melanotheron heudelotii is an ecologically appealing model as it shows exceptional adaptive capacities, especially with regard to salinity. In spite of this, this species is devoid of genomic resources, which impedes the understanding of such remarkable features. De novo assembly of transcript sequences produced by next-generation sequencing technologies offers a rapid approach to obtain expressed gene sequences for non-model organisms. It also facilitates the development of quantitative real-time PCR (qPCR) assays for analysing gene expression under different environmental conditions. Nevertheless, obtaining accurate and reliable qPCR results from such data requires a number of validations prior to interpretation.... |
| Tipo: Text |
Palavras-chave: Gene expression; Non-model organism; Reference gene; Salinity; Spermatogenesis; Tilapia. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00171/28255/26625.pdf |
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| Martins,M. R. F. B; Silva,P. J. R; Martins,B. B; Zoca,S. M; Freitas,M. A; Batista,T. C. |
| In order to study the morphological changes that occur in cells of the testes of isogenic black mouse C57BL/6/Uni into three periods during spermatogenetic used 15 mice divided into 3 groups of 5 animals with 40, 50 and 60 days of age. The mice were sacrificed and weighed. Then weighed and measured the testicles, to be processed finalmete histologically and stained with HE, PAS and Masson Massom-H and evaluated under light microscopy. Was observed in group I with 40 days of age in the seminiferous tubules had a lumen with sparse small amount of interstitial tubular cells. In the seminiferous epithelium were identified type A spermatogonia, intermediate and B, which occupied the compartment adbasal and intermingled with these cells was observed in... |
| Tipo: Journal article |
Palavras-chave: Spermatogenesis; Mouse; Testes. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000100052 |
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| Maia,Bernardo Porto; Silveira,Jéssica de Oliveira; Borges,Mariana Garcia Lisboa; Pereira,Danilo Ales Ponzi; Pinheiro,Julyara Lima; Freitas,Jofre Jacob da Silva; Kietzer,Katia Simone. |
| Androgenic anabolic steroids (AAS) are artificial testosterone analogues, used as medicine in chronic diseases, because they increase protein synthesis generating muscle hypertrophy. Its effect has caught the attention of athletes and gym users, thus their consumption has become epidemic, due to easy marketing, the immediate results and the false impression that it doesn't carry health risks. Such risks may globally harm the body. This study aims to investigate the influence on spermatogenesis of using nandrolone decanoate with or without physical training. Twenty-four rats, divided into four groups were used: sedentary group (SG), sedentary on steroids group (SSG), trained group (TG) and trained on steroids group (TSG). The animals were trained on... |
| Tipo: Journal article |
Palavras-chave: Spermatogenesis; Anabolic agents; Nandrolone; Exercise; Fertility. |
| Ano: 2015 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022015000300046 |
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| Bustos-Obregón,Eduardo; González-Hormazábal,Patricio. |
| Organophosphorous pesticides are a healh problem worldwide, mostly for agroworkers, which are around 2600 millions of person in the year 2001. Little is known about male reproductive damage elicited by these chemicals. The acute effects of malathion (single intraperitoneal injection), 1/12 the LD50 to mice were analyzed at 1, 8, 16, 35 and 40 days after injection, in terms of spermatogenic cell proliferation and apoptosis and of Sertoli cell compromise as revealed by immunocytochemical detection of CK-18 (cytokeratin). The micronuclei test was done to assess for genotoxic activity of the agropesticide. The results showed decreased germ cell number followed by compensatory spermatogonial proliferation by 16 days (as shown by thymidine-H3 testicular uptake),... |
| Tipo: Journal article |
Palavras-chave: Spermatogenesis; Organophosphorous agropesticides; Proliferation; Apoptosis. |
| Ano: 2003 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000200010 |
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| Espinoza-Navarro,O; Vilaxa,A; Granifo,L; Rojas,S; Rodríguez,H. |
| Boric acid and borate are abundant in the nature and widely used in industrial, agricultural and cosmetology applications. However it is informed as toxic in a high dose. Residents of Arica-Chile with boron-rich geology deposits are regularly exposed to higher levels in drinking water in concentrations of 6. 0 and 12.0 mg/L. The objective of this study was to analyze the damage in the masculine reproductive apparatus of mouse CF1, exposed to boric acid in concentrations of 0.54; 2.0-6.0 and 12.0 mg/L, after 60 days of treatment. Male gonad segments were fixed in Bouin's solution for 6 hours and embedded in paraffin, longitudinal sections (5 mm) were processed for histology using the hematoxyline/eosin method. The results observed in treated animals... |
| Tipo: Journal article |
Palavras-chave: Boric acid; Spermatogenesis; Testicular atrophy; Mouse. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000200017 |
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| Rolando,A.; Romanini,M. C.; Mugnaini,M. T.; Bozzo,A.; Pastorino,I.; Soñez,C. A.. |
| Spermatogenesis and the spermatozoon ultrastructure follow a common pattern in terrestrial oligochaetes. However, many of their characteristics are distinctive of each species and they are of great importance for phylogenetic and taxonomic studies. Germinal cells were studied through conventional electronic microscope techniques in order to go deep on spermatogenesis, especially on the mature spermatozoon ultrastructure of Eisenia foetida. Spermatogenesis occurs in seminal vesicles and follows a pattern comparable to that of some other oligachaetes species. Spermatozoon ultrastructural details revealed that it is a filiform cell, with the acrosome placed in anterior position, followed by the nucleus, the midpiece and the tail which flagellum has a 9+2... |
| Tipo: Journal article |
Palavras-chave: Oligochaeta; Spermatogenesis; Ultrastructure; Spermatozoa; Eisenia. |
| Ano: 2007 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022007000200005 |
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| Martins,M. R. F. B; Silva,P. J. R; Martins,B. B; Zoca,S. M; Freitas,M. A; Batista,T. C. |
| In order to study the morphological changes that occur in cells of the testes of isogenic black mouse C57BL/6/Uni into three periods during spermatogenetic used 15 mice divided into 3 groups of 5 animals with 40, 50 and 60 days of age. The mice were sacrificed and weighed. Testicles were weighed and measured, and histologically processed and stained with HE, PAS and Masson Massom-H and evaluated under light microscopy. It was observed that group I with 40 days of age in the seminiferous tubules had a lumen with sparse small amount of interstitial tubular cells. In the seminiferous epithelium type A spermatogonia, intermediate and B were identified, which occupied the compartment adbasal and intermingled with these cells in spermatocytes I in Pachytene and... |
| Tipo: Journal article |
Palavras-chave: Spermatogenesis; Mouse; Testes. |
| Ano: 2012 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022012000200022 |
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| Hojati,Vida; Keshavarz,Seyed Mohammad; Behniafar,Reza; Shiravi,Abdolhossein. |
| The Caspian Green Lizard, Lacerta strigata Eichwald, 1831, is a member of the Lacertidae family distributed in the Caspian coast of northern Iran. The specimens were found in coastal dunes, hilly areas and pastureland containing some shrubby cover. In this research, the reproductive biology of this species was studied from April 20 to September 5, 2013. Forty eight adult specimens including 24 females and 24 males were collected by hand at midday from three sampling stations (Goharbaran, Ab-Bandansar and Ab-Bandankesh) of Sari County, located in Mazandaran Province of Iran. Ovaries and testes were removed and processed for histological and morphometric studies. In females, there were 617 follicles in each ovary. Totally, 610 oviductal eggs were observed... |
| Tipo: Journal article |
Palavras-chave: Lacertidae; Caspian green lizard; Oogenesis; Spermatogenesis; Caspian coast. |
| Ano: 2016 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022016000200005 |
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| Schwarz,Franciele Cristina; Mansano,Naira da Silva; Chies,Agnaldo Bruno; Viani,Gustavo Arruda; Spadella,Maria Angélica. |
| Radiotherapy is a source of human exposure to ionizing radiation. This pure energy causes deleterious effects on tissues, which result from oxidative stress, a phenomenon in which there is the participation of the Renin-Angiotensin System (RAS). The male genital organs are extremely radiosensitive and the action of radiation in the testes can significantly affect spermatogenesis. In search of potential radioprotective for male genital system, this study investigated whether the AT1 receptor antagonists minimize radiation-induced damage to reproductive tissues, by decreasing oxidative stress. Male Wistar rats were divided into six groups: 0 Gray (Gy) (control), 5 Gy (single dose in the scrotal area), telmisartan, losartan, 5Gy+telmisartan and 5Gy+losartan.... |
| Tipo: Journal article |
Palavras-chave: Ionizing radiation; Testicular ultrastructure; Spermatogenesis; Renin-angiotensin system; Oxidative stress; Angiotensin II type 1 receptor blockers. |
| Ano: 2017 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022017000300005 |
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| Registros recuperados: 51 | |
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