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	Provedor de dados BJM (2) Autor Contiero,Jonas (1) Ferreira-Nozawa,M.S. (1) Freitas,Telma Luisa de (1) Guimarães,L.H.S. (1) Jorge,J.A. (1) Monti,Rubens (1) Polizeli,M.L.T.M. (1) Rezende,J.L. (1) Terenzi,H.F. (1) Mais... Palavra-chave Starch hydrolysis (2) Amylase (1) Bacillus alkalophilic CGII (1) Beta-cyclodextrin (1) CGTase (1) Enzyme activity (1) Glucoamylase (1) Scytalidium thermophilum (1) Thermostable enzyme (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2008 (1) 2004 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Mycelial glucoamylases produced by the thermophilic fungus Scytalidium thermophilum strains 15.1 and 15.8: purification and biochemical characterization BJM Ferreira-Nozawa,M.S.; Rezende,J.L.; Guimarães,L.H.S.; Terenzi,H.F.; Jorge,J.A.; Polizeli,M.L.T.M.. Two strains (15.1 and 15.8) of the thermophilic fungus Scytalidium thermophilum produced high levels of intracellular glucoamylases, with potential for industrial applications. The isoform I of the glucoamylase produced by 15.1 strain was sequentially submitted to DEAE-Cellulose and CM-Cellulose chromatography, and purified 141-fold, with 5.45% recovery. The glucoamylase of strain 15.8 was purified 71-fold by CM-Cellulose and Concanavalin A-Sepharose chromatography, with 7.38% recovery. Temperature and pH optima were in the range of 50-60ºC and 5.0-6.0, respectively, using starch and maltose as substrates. The glucoamylase of S. thermophilum 15.8 was more stable (t50 > 60 min) than that of S. thermophilum 15.1 (t50= 11-15 min), at 60ºC. The glucoamylase... Tipo: Info:eu-repo/semantics/article Palavras-chave: Glucoamylase; Amylase; Scytalidium thermophilum; Thermostable enzyme; Starch hydrolysis. Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000200027 Production of CGTase by a Bacillus alkalophilic CGII strain isolated from wastewater of a manioc flour industry BJM Freitas,Telma Luisa de; Monti,Rubens; Contiero,Jonas. GCTase production by a new strain of Bacillus alkalophilic CGII isolated from Brazilian wastewater of manioc flour industry was examined. The growth medium used was composed by 1.5% starch, 1.5% nitrogen and 1% Na2CO3. Higher activity was obtained with starch, maltodextrin and galactose. When glucose was added to the medium, no enzyme production was observed. High enzyme activity and growth were reached when aeration was increased (88.6 U/mL). The enzyme characterization showed an optimum pH and temperature 8.0 and 55ºC for starch hydrolyses, respectively. Mg+ and Ca++ showed small activation; however, Hg+ and Cu+ showed a strong enzyme inhibition. Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacillus alkalophilic CGII; CGTase; Beta-cyclodextrin; Starch hydrolysis; Enzyme activity. Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200015 Registros recuperados: 2 Primeira ... 1 ... Última

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