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| Silva,Maria Cristina Mattar da; Mello,Luciane Vieira; Coutinho,Marise Ventura; Rigden,Daniel John; Neshich,Goran; Chrispeels,Maarten John; Grossi-de-Sá,Maria Fátima. |
| Despite the presence of a family of defense proteins, Phaseolus vulgaris can be attacked by bruchid insects resulting in serious damage to stored grains. The two distinct active forms of a-amylase inhibitors, a-AI1 and a-AI2, in P. vulgaris show different specificity toward a-amylases. Zabrotes subfasciatus a-amylase is inhibited by a-AI2 but not by a-AI1. In contrast, porcine a-amylase is inhibited by a-AI1 but not by a-AI2. The objective of this work was to understand the molecular basis of the specificity of two inhibitors in P. vulgaris (a-AI1 and a-AI2) in relation to a-amylases. Mutants of a-AI2 were made and expressed in tobacco plants. The results showed that all the a-AI2 mutant inhibitors lost their activity against the insect a-amylases but none... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Phaseolus vulgaris; A-amylase inhibitors; Inhibitor specificity; Site directed mutagenesis; Structural modeling. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2004000300001 |
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| Vieira Neto,José Gabriel; Soriano,Julio. |
| ABSTRACT Widely disseminated in both national and international scenarios, greenhouses are agribusiness solutions which are designed to allow for greater efficiency and control of the cultivation of plants. Bearing this in mind, the construction of greenhouses should take into consideration the incidence of wind, and other such aspects of comfort and safety, and ensure they are factored into the design of structural elements. In this study, we evaluated the effects of pressure coefficients established by the European standard EN 13031-1 (2001) and the Brazilian standard ABNT (1988), which are applicable to the structures of greenhouses with flat roofs, taking into account the following variables: roof slope, external and internal pressure coefficients and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Wind action; Pitched roof; Structural modeling. |
| Ano: 2016 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000200097 |
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