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Frequency of microorganisms isolated at different stages of lactation and milk production loss associated with somatic cell count and to mastitis-causing pathogens Arq. Bras. Med. Vet. Zootec.
Costa,H.N.; Lage,C.F.A.; Malacco,V.M.R.; Belli,A.L.; Carvalho,A.U.; Facury,E.J.; Molina,L.R..
ABSTRACT The aim of this study was to evaluate the frequency of isolation of agents causing subclinical mastitis in a herd and to estimate production losses associated with SCC> 200,000cells/mL. Three 7-day interval microbiological cultures were performed in all lactating animals from the same farm that was evaluated from June to July. To evaluate the negative and positive isolation frequencies between the lactation phases, a Chi-square test was performed. Simple linear regressions were performed to evaluate the lactation curve of animals grouped by pathogens isolated from negative cows in the microbiological culture and with SCC <200,000cells/mL. To estimate the production losses between the groups, regression coefficients were used. Results found...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Subclinical mastitis; Environmental; Contagious; Milk quality.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200393
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New method for early detection of two random amplified polymorphic DNA (RAPD) groups of Staphylococcus aureus causing bovine mastitis infection in Paraná State, Brazil BABT
Gonçalves,Dicezar; Gabriel,Jane Eire; Madeira,Humberto Maciel França; Schühli,Guilherme Schnell e; Vicente,Vânia Aparecida.
The aim of this work was to develop a fast and accurate molecular approach to allow early detection of two RAPD groups of S. aureus causing bovine mastitis. Seventy five S. aureus isolates from infected animals were characterized by RAPD. Genomic fragments isolated from the unique bands present in either group were cloned and sequenced. Based on the DNA sequences, specific primers were designed to allow for the simultaneous detection of either group by multiplex PCR of S. aureus DNA isolated from clinical and subclinical bovine mastitis. Results showed that these proposed primers set could be used to detect various clinical and subclinical S. aureus isolates as well as the detection of the microorganism in bulk milk. Their use as a specific method for...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Staphylococcus aureus; Molecular markers; Subclinical mastitis; Clinical mastitis; Mastitis detection.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200014
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