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| Santos,Silvia; Baruque-Ramos,Júlia; Tanizaki,Marta Massako; Lebrun,Ivo; Schenkman,Rocilda Perazzini Furtado. |
| Meningococcal disease is an important cause of death and morbidity throughout the world. Nearly 330,000 cases and 35,000 deaths occur yearly. Neisseria meningitidis, serogroup B strain N.44/89, is prevalent in Brazil. Its outer membrane vesicles (OMV) with iron regulated proteins (IRP) are released to the culture medium and are used as antigen for vaccine production. In order to have knowledge about the kinetic parameters, especially the final OMV concentration values, 20-h batch cultivations were carried out in Catlin medium with iron restriction. Process conditions comprised: 7 L bioreactor, 36ºC, 0.5 atm, overlay air flowrate of 1 L/min, agitation varying from 250 rpm to 850 rpm and dissolved oxygen control set at 10% of saturation condition. Biomass... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neisseria meningitidis; Outer membrane vesicles; Culture medium; Vaccine; Submerged batch cultivation. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400016 |
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| Leal,Maria Betania Batista; Baruque-Ramos,Júlia; Hiss,Haroldo; Paz,Marcelo Fossa da; Sakai,Maria Cristina; Vassoler,Umbelina Macedo; Arauz,Luciana Juncioni de; Raw,Isaías. |
| The influences of the L-asparagine and glycerol initial concentrations in Sauton medium on the productivities of biomass and colony forming units were studied. The submerged batch cultivations of Mycobacterium bovis were carried out in a 20 L bioreactor. The L-asparagine and glycerol initial concentrations of 4.54 g/L and 25 mL/L, respectively, corresponded to the best biomass productivity, namely 2.5 g/L.day. On the other hand, the concentrations of 2.27 g/L and 25 mL/L, respectively, led to the highest productivity in terms of colony forming units, namely 2.7·10(6) colonies/mg.day. In addition, by means of the relative consumption analysis of L-asparagine and glycerol (50 and 26% respectively), it was concluded that the concentrations of such components... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BCG; Mycobacterium bovis; Culture medium; Vaccine; Submerged batch cultivation. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000300013 |
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