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How to construct and use a simple device to prevent the formation of precipitates when using Sudan Black B for histology Acta Botanica
Oliveira,João Marcelo Santos de.
The present work aims to demonstrate the stages of fabrication and use of a simple device to avoid the formation or fixation of precipitates from Sudan Black B solution on tissues. The device consists of four coverslip fragments attached to a histology slide, which serve as points of support for the histological slide under analysis. To work properly, the histology slide with the sections should be placed with the sections facing downwards the device. A small space between the device and the histology slide is thereby created by the height of the coverslip fragments. When Sudan Black B is applied, the solution is maintained within the edges of the device and evaporation is minimized by the small space, thereby reducing the consequent formation of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Histochemical tests; Histology; Lipids; Plant anatomy; Sudan Black B.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000400489
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