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Removal of the cortical projections alters expression of NOS in the different cell types of the superficial layers of the superior colliculus in rats Anais da ABC (AABC)
TENÓRIO,FRANK; GIRALDI-GUIMARÃES,ARTHUR; MENDEZ-OTERO,ROSALIA.
Nitric oxide has several biological roles and nitric oxide synthase (NOS) is expressed in the nervous system, and co-localizes with NADPH-diaphorase. The superficial layers of the superior colliculus (SC), which receive retinal and cortical inputs, present NADPH-d staining in a sub-population of neurons that include all cell types. We have previously shown, by NADPH-diaphorase, that eye enucleation alters the intracellular distribution of NOS. Here, we studied the effect of cortical ablation on NOS expression by neurons in collicular superficial layers. Our results show that cortical ablation alters the proportion of different NOS-positive cell types, but not the intracellular distribution of the enzyme.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Superior colliculus; Nitric oxide; Visual cortex; NADPH-diaphorase.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652002000400008
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Expression of neuronal nitric oxide synthase in the developing superficial layers of the rat superior colliculus BJMBR
Giraldi-Guimarães,A.; Bittencourt-Navarrete,R.E.; Mendez-Otero,R..
We investigated the level of expression of neuronal nitric oxide synthase (nNOS) in the retinorecipient layers of the rat superior colliculus during early postnatal development. Male and female Lister rats ranging in age between the day of birth (P0) and the fourth postnatal week were used in the present study. Two biochemical methods were used, i.e., in vitro measurement of NOS specific activity by the conversion of [³H]-arginine to [³H]-citrulline, and analysis of Western blotting immunoreactive bands from superior colliculus homogenates. As revealed by Western blotting, very weak immunoreactive bands were observed as early as P0-2, and their intensity increased progressively at least until P21. The analysis of specific activity of NOS showed similar...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Superior colliculus; Nitric oxide synthase; Development; Retinotectal projection.
Ano: 2004 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2004000600013
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NMDA receptor blockade alters the intracellular distribution of neuronal nitric oxide synthase in the superficial layers of the rat superior colliculus BJMBR
Bittencourt-Navarrete,R.E. de; Nascimento,I.C.do; Santiago,M.F.; Mendez-Otero,R..
Nitric oxide (NO) is a molecular messenger involved in several events of synaptic plasticity in the central nervous system. Ca2+ influx through the N-methyl-D-aspartate receptor (NMDAR) triggers the synthesis of NO by activating the enzyme neuronal nitric oxide synthase (nNOS) in postsynaptic densities. Therefore, NMDAR and nNOS are part of the intricate scenario of postsynaptic densities. In the present study, we hypothesized that the intracellular distribution of nNOS in the neurons of superior colliculus (SC) superficial layers is an NMDAR activity-dependent process. We used osmotic minipumps to promote chronic blockade of the receptors with the pharmacological agent MK-801 in the SC of 7 adult rats. The effective blockade of NMDAR was assessed by...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Superior colliculus; Nitric oxide synthase; NMDA receptor; Postsynaptic density targeting.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2009000200007
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