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Examining new phylogenetic markers to uncover the evolutionary history of early-diverging fungi: comparing MCM7, TSR1 and rRNA genes for single- and multi-gene analyses of the Kickxellomycotina Naturalis
Tretter, E.D.; Johnson, E.M.; Wang, Y.; Kandel, P.; White, M.M..
The recently recognised protein-coding genes MCM7 and TSR1 have shown significant promise for phylogenetic resolution within the Ascomycota and Basidiomycota, but have remained unexamined within other fungal groups (except for Mucorales). We designed and tested primers to amplify these genes across early-diverging fungal clades, with emphasis on the Kickxellomycotina, zygomycetous fungi with characteristic flared septal walls forming pores with lenticular plugs. Phylogenetic tree resolution and congruence with MCM7 and TSR1 were compared against those inferred with nuclear small (SSU) and large subunit (LSU) rRNA genes. We also combined MCM7 and TSR1 data with the rDNA data to create 3- and 4-gene trees of the Kickxellomycotina that help to resolve...
Tipo: Article / Letter to the editor Palavras-chave: DNA replication licensing factor; Harpellales; Kickxellomycotina; MCM7; MS277; MS456; Ribosomal biogenesis protein; Trichomycetes; TSR1; Zygomycota.
Ano: 2013 URL: http://www.repository.naturalis.nl/record/532223
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