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Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks Electron. J. Biotechnol.
Xiao,Anfeng; Huang,Yufeng; Ni,Hui; Cai,Huinong; Yang,Qiuming.
Background A sequential statistical strategy was used to optimize tannase production from Aspergillus tubingensis using tea stalks by solid-state fermentation. Results First, using a Plackett-Burman design, inoculum size and incubation time (among seven tested variables) were identified as the most significant factors for tannase yield. The effects of significant variables were further evaluated through a single steepest ascent experiment and central composite design with response surface analysis. Under optimal conditions, the experimental value of 84.24 units per gram of dry substrate (U/gds) closely matched the predicted value of 87.26 U/gds. Conclusions The result of the statistical approach was 2.09 times higher than the basal medium (40.22 U/gds)....
Tipo: Journal article Palavras-chave: Aspergillus tubingensis; Process optimization; Response surface methodology; Tea stalks; Tannase fermentation.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300001
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