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| Gong,Zhen; Liu,Jianyun; Xie,Xin; Xu,Xiaoyuan; Wu,Ping; Li,Huimin; Wang,Yaqin; Li,Weidong; Xiong,Jianjun. |
| Abstract The ubiquitin-specific protease 22 (USP22) is an oncogene and its expression is upregulated in many types of cancer. In the nucleus, USP22 functions as one subunit of the SAGA to regulate gene transcription. However, the genome-wide USP22 binding sites and its direct target genes are yet clear. In this study, we characterized the potential genomic binding sites of UPS22 and GCN5 by ChIP-seq using specific antibodies in HeLa cells. There were 408 overlapping putative target genes bound by both USP22 and GCN5. Motif analysis showed that the sequences bound by USP22 and GCN5 shared two common motifs. Gene ontology (GO) and pathway analysis indicated that the genes targeted by USP22 and GCN5 were involved in different physiological processes and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: USP22; Target genes; ChIP-seq; Knockdown; RNA-seq. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000300488 |
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